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Successive construction of cellulase hyperproducers of Trichoderma using hyperpolyploids

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Abstract

When the swollen conidia of Trichoderma reesei QM 6a are treated with 0.1% (w/v) colchicine solution, huge autopolyploid nuclei can be formed in those swollen conidia. When a mycelial mat derived from such a conidum is treated with a haploidizing reagent, benomyl, many fan-shaped sectors are produced from the colony, and cellulase hyperproducers are selected from conidia on the colony. When colchicine and benomyl treatments are repeated on cellulase hyperproducers, new hyperproducers can be constructed successively and systematically. Moreover, when conidia derived from autopolyploids are treated with ethylmethanesulfonate solution, another type of cellulase hyperproducers (polyploids) can be obtained.

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Authors and Affiliations

  1. Department of Food Science and Technology, Faculty of Horticulture, Minamikyushu University, Takanabe, 884-0003, Miyazaki, Japan

    Hideo Toyama & Nobuo Toyama

Authors
  1. Hideo Toyama
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  2. Nobuo Toyama
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Correspondence to Hideo Toyama.

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Toyama, H., Toyama, N. Successive construction of cellulase hyperproducers of Trichoderma using hyperpolyploids. Appl Biochem Biotechnol 84, 419–429 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:419

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  • DOI: https://doi.org/10.1385/ABAB:84-86:1-9:419

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