Abstract
Micronucleus-like structures were produced in Trichoderma reesei only when 0.1% colchicine treatment was used to enhance nuclear division. The average DNA content of these ‘small nuclei’ was 30% that of the normal nuclei, indicating that they were aneuploid nuclei. Such small nuclei may be useful in transferring small amounts of DNA into protoplasts.
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The authors are with the Department of Food Technology, Faculty of Horticulture, Minamikyushu University, Takanabe-Cho, Hibarigaoka, Miyazaki 884, Japan;
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Toyama, H., Toyama, N. Factors affecting formation of micronucleus-like structures after colchicine treatment of Trichoderma reesei . World Journal of Microbiology & Biotechnology 11, 326–329 (1995). https://doi.org/10.1007/BF00367110
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DOI: https://doi.org/10.1007/BF00367110