Abstract
It is an accepted fact that ethanol production from lignocellulosic materials is not economical as yet because of the high cost of cellulase production. To reduce the cost of cellulase production, lignocellulosic material (wheat straw [WS]), a comparatively much cheaper substrate, was used instead of costly substrates (pure cellulose or lactose). A pan bioreactor was developed for solid-state fermentation (SSF) that required a small capital investment. High yields of complete cellulase system were obtained compared to that in the liquid-state fermentation (LSF) from WS, when treated with 4.25% NaOH at 121 °C for 1 h and mixed with Mandels’ medium. A complete cellulase system is defined as one in which the ratio of β-glucosidase activity to filter paper activity in the enzyme solution is close to 1.0. The cellulase system derived from SSF using the pan bioreactor gave more than 85% hydrolysis of delignified WS. The prototype pan bioreactor requires further improvements so that optimum quantity of substrate can be fermented to obtain high yields of complete cellulase system per unit space. The SSF process provides a means for the production of complete cellulase system for the economical bioconversion of renewable biomass into ethanol.
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References
Chahal, D. S., Chahal, P. S., André, G., and Ishaque, M. (1987), inSixth Canadian Bioenergy R&D Seminar, Stiassny, Z. Z., ed., Elsevier Applied Science, New York, pp. 306–310.
Chahal, D. S. (1982), inEnzymatic Hydrolysis of Cellulose: State-of-the-Art, Division of Energy Research and Development, National Research Council Canada, Ottawa, Canada, pp. 74–95.
Chahal, D. S., Mcguire, S., Pikor, H., and Noble, G. (1982),Biomass 2, 127–138.
Chahal, D. S. (1985),Appl. Environ. Microbiol. 49, 205–210.
Stockton, B. C., Mitchell, D. J., Grohmann, K., and Himmel, M. E. (1991),Biotechnol. Lett. 13, 57–62.
King, K. W. and Vessal, M. I. (1969),Adv. Chem. Ser. 95, 7–25.
Wood, T. M. (1981),Biochem. J. 121, 353–362.
Ghose, T. K. and Das, K. (1971),Adv. Biochem. Eng. 1, 55–62.
Gritzali, M. and Brown, R. D. (1979),Adv. Chem. Ser. 181, 237–260.
Ryu, D. and Mandels, M. (1980),Enzyme Microb. Technol. 2, 91–102.
Mandels, M. and Weber, J. (1969),Adv. Chem. Ser. 95, 391–414.
Mandels, M., Andreotti, R., and Roche, C. (1976),Biotechnol. Bioeng. Symp. 6, 21–33.
Ghose, T. K. (1987),Pure and Appl. Chem. 59, 257–268.
Toyama, N. and Ogawa, K. (1972),Fermentation Technology Today. Society of Fermentation Technology, Japan, pp. 743–757.
Box, G. E. P., Hunter, W. G. and Hunter, J. S. (1978),Statistics for Experimenters, An Introduction to Design, Data Analysis, and Model Building, Wiley, New York, pp. 510–539.
Box, G. E. P. and Hunter, J. S. (1957),Ann. Math. Stat. 28, 1–25.
Schmidt, S. R. and Launsby, R. G. (1992),Understanding Industrial Designed Experiments, 3rd ed. Air Academy Press, Colorado Springs, CO, pp. 7.1–7.17.
Cochran, W. C. and Cox, G. M. (1968),Experimental Designs, 2nd ed., Wiley, New York, pp. 335–375.
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Chahal, P.S., Chahal, D.S. & Lê, G.B.B. Production of cellulase in solid-state fermentation withTrichoderma reesei MCG 80 on wheat straw. Appl Biochem Biotechnol 57, 433–442 (1996). https://doi.org/10.1007/BF02941724
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DOI: https://doi.org/10.1007/BF02941724