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Lignin peroxidase production by Streptomyces viridosporus T7A

Use of corn oil as a carbon source

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Abstract

Lignin peroxidase (LiP) production cost should be reduced to justify its use in the control of environmental pollution. In this work, we studied the enzyme production by Streptomyces viridosporus T7A using glucose or corn oil as a carbon source having 0.65% yeast extract as a nitrogen source. Enzyme activity, observed using either 0.65% glucose or corn oil at 0.1, 0.5, and 1.0% concentration, was 300, 150, 300, and 200 U/L, respectively. Although higher enzyme activity was obtained in both media containing 0.65% glucose and 0.5% corn oil, the use of corn oil resulted in a better LiP stability. When combined carbon sources were used, higher values of enzyme activity (360, 350, and 225 U/L) were observed in media with 0.65% glucose and supplemented with 0.1, 0.5, and 1.0% corn oil, respectively. Although the presence of both glucose and 0.5% corn oil is favorable for LiP production, satisfactory results in terms of enzyme production and stability could be also observed using 0.5% corn oil as a sole carbon source, which may lead to reduced production costs of the LiP enzyme.

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Authors and Affiliations

  1. Departamento de Bioquímica, IQ, CT, Universidade Federal do Rio de Janeiro, 21949-900, RJ, Brazil

    Leda M. F. Gottschalk, Jacyara M. B. Macedo & Elba P. S. Bon

  2. Departamento de Bioquímica, IBRAG, Universidade do Estado do Rio de Janeiro, 20551-013, RJ, Brazil

    Jacyara M. B. Macedo

Authors
  1. Leda M. F. Gottschalk
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  2. Jacyara M. B. Macedo
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  3. Elba P. S. Bon
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Correspondence to Elba P. S. Bon.

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Gottschalk, L.M.F., Macedo, J.M.B. & Bon, E.P.S. Lignin peroxidase production by Streptomyces viridosporus T7A. Appl Biochem Biotechnol 79, 771–778 (1999). https://doi.org/10.1385/ABAB:79:1-3:771

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  • DOI: https://doi.org/10.1385/ABAB:79:1-3:771

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