Abstract
Saccharification and fermentation of cassava (Manihot esculenta) bagasse was carried out in a single step for the production of L-(+)-lactic acid by Lactobacillus casei and Lactobacillus delbrueckii. Using 15.5% w/v of cassava bagasse as the raw material, a maximum starch to lactic acid conversion of 96% was obtained with L. casei with a productivity rate of 1.40mg/mL·h and maximum yield of 83.8 mg/mL. It was 94% with L. delbrueckii with a productivity rate of 1.36 mg/mL·h. and maximum yeild of 81.9 mg/mL. Supplementation of bagasse with 0.01% w/v MnCl2 showed positive influence on the lactic acid production by L. casei.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dimerci, A., Pometto III, A. L., and Johnson, K. E. (1993) Appl. Environ. Microbiol. 59, 203–207.
Anuradha, R., Suresh, A. K., and Venkatesh, K. V. (1999) Proc. Biochem. 35, 367–375.
Pandey, A., Soccol, C. R., Jose, A., Rodriguez-loen, and Nigam P. (2001), Solid State Fermentation in Biotechnology: Fundamentals and Applications, Asiatech Publishers, New Delhi.
Yun, J., Wee, Y., and Ryu, H. (2003) Enzyme Microb. Technol. 33, 416–423.
Yun, J., Wee, Y., Kim, J., and Ryu, H. (2004) Biotechnol. Lett. 18, 1613–1616.
Naveena, B. J., Vishnu, C., Altaf, Md., and Reddy, G. (2003) J. Sci. Ind. Res. 62, 453–456.
Linko, Y. Y. and Javanainen P. (1996) Enzyme Microb. Technol. 19, 118–123.
Abe, S. and Takagi, M. (1991) Biotechnol. Bioeng. 37, 93–96.
Venkatesh, K. V. (1997) Biores. Technol. 62, 91–98.
Yu, R. U. and Hang, Y. D. (1989) Biotechnol., Lett. 11, 597–600.
Naveena B. J. (2004), Ph D Thesis, Osmania University, Hyderabad, India.
Pandey, A., Soccol, C. R., Nigam, P., Soccol, V. T., Vandenbergh, L. P. S., and Mohan, R. (2000) Biores. Technol. 74, 81–87.
Woiciechowski, A. L., Nistsche, S., Pandey, A. and Soccol, C. R. (2002) Braz. Archives Biol. Technol. 45, 393–400.
Carta, F. S., Soccol, C. R., Ramos, L. P., and Fontana, J. D. (1999) Biores. Technol. 68, 23–28.
Barker, S. B. and Summerson, W. H. (1941) J. Biol. Chem. 138, 535–554.
Miller, G. L. (1959) Anal. Chem. 31 426–429.
Nampoothiri, K. M., Singhania, R. R., Sabarinath, C., and Pandey, A. (2003) Proc. Biochem. 38, 1513–1519.
Hujanen, M. and Linko, Y. Y. (1994) Biotechnol. Tech. 8, 325–333.
Hofvendahl, K. and Hahn-hagerdal, B. (2000) Enzyme. Microb. Technol. 26, 87–107.
Nancib, A., Nancib, N., Meziane-cherif, D., Boudenbir, A., Fick, M., and Boudrant, J. (2005) Biores. Technol. 96, 63–67.
Frobisher, M., Hinsdill, R. D., Crabtree, K. T., and Goodheart, C. R. (1974), in Fundamentals of Microbiology, WB Saunders Company, Philadelphia
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
John, R.P., Madhavan Nampoothiri, K. & Pandey, A. Simultaneous saccharification and fermentation of cassava bagasse for l-(+)-lactic acid production using Lactobacilli . Appl Biochem Biotechnol 134, 263–272 (2006). https://doi.org/10.1385/ABAB:134:3:263
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/ABAB:134:3:263