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Hollow-Fiber Ultrafiltration then Centrifugation for LC Analysis of Water-Soluble Sucrose in a Water-Soluble High-Molecular-Mass Gel Matrix

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Abstract

A novel, simple, and economic method, hollow-fiber ultrafiltration followed by centrifugation, has been developed for separation of viscid substances with similar properties except for different molecular weight. The experimental assembly needed only a U-shaped hollow fiber containing the sample solution with both ends of the fiber connected to two syringe needles. Ultrafiltration was performed by the action of centrifugal force. Concentration polarization was overcome and no phase transition occurred. Satisfactory recovery was achieved.

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang, People’s Republic of China

    Junmei Li, Ye Jiang & Ting Sun

  2. Department of Research and Development, Science and Technology General Company, Hebei Medical University, 9 North Physical Street, Shijiazhuang, People’s Republic of China

    Lijuan Kang

Authors
  1. Junmei Li
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  2. Ye Jiang
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  3. Ting Sun
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  4. Lijuan Kang
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Correspondence to Ye Jiang.

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Li, J., Jiang, Y., Sun, T. et al. Hollow-Fiber Ultrafiltration then Centrifugation for LC Analysis of Water-Soluble Sucrose in a Water-Soluble High-Molecular-Mass Gel Matrix. Chroma 70, 1023–1030 (2009). https://doi.org/10.1365/s10337-009-1257-y

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