Abstract
The diazo component of o-hydroxy heterocyclic azo compounds showed a remarkable effect on the retention of their neutral complexes of iron and nickel as a precolumn derivatizing reagent in the separation of metal ions by RP-HPLC using an octadecyl-bonded silica gel column. High resolutions of iron and nickel complexes were obtained with pyrimidyl derivatives. Acetonitrile-water or acetone-water was more suitable as a mobile phase than alcohol-water with regard to the separation of these metal ions. Thermodynamic study showed that the difference in transfer enthalpies of iron and nickel complexes from the mobile phase to the stationary phase dominated the resolution of two complexes with pyrimidyl compounds. High resolutions obtained by use of pyrimidyl compounds were ascribed to selectively enhanced retention of the iron complexes from the comparison with the retentions of the zinc complexes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dolezal J, Sommer L (1995) Scr Chem 24:73–89
Sommer L, Dolezal J (1997) Scr Chem 26:65–92
Yasui T, Yuchi A, Yamada H, Wada H (1994) J Chromatogr A 659:359–365. doi:10.1016/0021-9673(94)85078-X
Inoue H, Ito K (1994) Microchem J 49:249–255. doi:10.1006/mchj.1994.1035
Niwa H, Yasui T, Ishizuki T, Yuchi A, Yamada H, Wada H (1997) Talanta 45:349–355. doi:10.1016/S0039-9140(97)00135-5
Niwa H, Yasui T, Yuchi A, Yamada H, Wada H (1997) J Chromatogr A 789:491–496. doi:10.1016/S0021-9673(97)00792-9
Miura J (1990) Anal Chem 62:1424–1428. doi:10.1021/ac00213a015
Yasui T, Ohnishi T, Mizuno Y, Ohata N, Yamada H (2003) Bunseki Kagaku 52:1121–1129. doi:10.2116/bunsekikagaku.52.1121
Anderson RG, Nickless G (1968) Analyst Camb UK 93:20–25
Singh I, Saini R (1994) Talanta 41:2173–2175. doi:10.1016/0039-9140(94)00176-6
Gaber M, Ayad MM, El-Sayed YSY (2005) Spectrochim Acta Part A Mol Biomol Spectrosc 62:694–702. doi:10.1016/j.saa.2005.02.039
Kawase A (1972) Anal Chim Acta 58:311–322. doi:10.1016/S0003-2670(72)80007-2
Nakagawa G, Wada H (1964) Nippon Kagaku Zasshi 85:202–206
Ohshita K, Wada H, Nakagawa G (1982) Anal Chim Acta 140:291–300. doi:10.1016/S0003-2670(01)95475-3
Nakagawa G, Wada H (1963) Nippon Kagaku Zasshi 84:639–641
Cole LA, Dorsey JG, Dill KA (1992) Anal Chem 64:1324–1327. doi:10.1021/ac00037a005
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yasui, T., Mizuno, Y., Maeda, K. et al. Selectively Enhanced Retention of Neutral Iron(II) Complex in RP-LC with o-Hydroxy Pyrimidylazo Compounds as Precolumn Derivatizing Reagent. Chroma 68, 387–391 (2008). https://doi.org/10.1365/s10337-008-0724-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-008-0724-1