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Simultaneous Determination of EDTA, Sorbic Acid, and Diclofenac Sodium in Pharmaceutical Preparations Using High-Performance Liquid Chromatography

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Abstract

A simple high-performance liquid chromatographic method for simultaneous determination of ethylenediaminetetraacetic acid (EDTA), sorbic acid, and diclofenac sodium was developed and validated. Separation was achieved on a C18 column (10 cm × 4.6 mm) using gradient elution. The mobile phase consisted of acetonitrile–ammonium dihydrogen phosphate buffer solution (0.01 M, pH = 2.5, containing 0.8% tetra-n-butyl ammonium hydroxide). The detector wavelength was set at 254 nm. Under these conditions, separation of three compounds was achieved in less than 10 min. The effect of two metal salts and metal concentration on peak area of EDTA was investigated. The pH effect on retention of EDTA and sorbic acid was studied. The method showed linearity for EDTA, sorbic acid, and diclofenac in the ranges of 2.5–100.0, 5.0–200.0, and 20.0–120.0 μg/mL, respectively. The within- and between-day relative standard deviations ranged from 0.52 to 1.94%, 0.50 to 1.34%, and 0.78 to 1.67% for EDTA, sorbic acid, and diclofenac, respectively. The recovery of EDTA, sorbic acid, and diclofenac from pharmaceutical preparation ranged from 96.0–102.0%, 99.7–101.5%, to 97.0–102.5%, respectively. To the best of our knowledge, this is the first report about simultaneous determination of EDTA, sorbic acid, and diclofenac.

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ACKNOWLEDGMENTS

This research work was supported by Bakhtar Bioshimi Pharmaceutical Company and Iran National Science Foundation (INSF). The author would like to thank Mr. Bahman Barazesh for the excellent and open collaboration.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran

    Rouhollah Heydari & Nasim Naleini

  2. Department of Chemistry, Faculty of Science, Razi University, Kermanshah, Iran

    Mojtaba Shamsipur

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  1. Rouhollah Heydari
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  2. Mojtaba Shamsipur
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  3. Nasim Naleini
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Correspondence to Rouhollah Heydari or Mojtaba Shamsipur.

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Heydari, R., Shamsipur, M. & Naleini, N. Simultaneous Determination of EDTA, Sorbic Acid, and Diclofenac Sodium in Pharmaceutical Preparations Using High-Performance Liquid Chromatography. AAPS PharmSciTech 14, 764–769 (2013). https://doi.org/10.1208/s12249-013-9962-0

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