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Simultaneous Determination of Diclofenac and Esomeprazole by Reversed phase Liquid Chromatography, Dual Wavelength and Derivative Spectrophotometry

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Abstract

Three methods were developed for simultaneous determination of diclofenac and esomeprazole in their binary mixtures. Method I is dual wavelength spectrophotometry which is based on measurement of absorbance difference ΔA (281.7–315 nm) for determination of diclofenac and ∆A (262.6–301.6 nm) for determination of esomeprazole. Method II is derivative spectrophotometry which is based on measuring 1D at 301.6 nm for determination of diclofenac and 2D at 299.5 nm for determination of esomeprazole. Linearity ranges were found to be 5–25 and 2‒10 μg/mL for diclofenac and esomeprazole, respectively, using methods I and II. Method III is reversed phase HPLC method which involves separation of diclofenac and esomeprazole on XBridgeTMC18 column using a mobile phase consisting of ethanol-phosphate buffer solution (pH 4, 60 : 40, v/v). The flow rate was 1.5 mL/min with ultraviolet detection at 290 nm. The calibration graphs are linear from 2 to 120 μg/mL for diclofenac and from 5 to 60 μg/mL for esomeprazole.

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REFERENCES

  1. British Pharmacopoeia, London: The Stationery Office, 2013.

  2. Moffat, A.C., Osselton, D., Widdop, B., and Watts, J., Clarke’s Analysis of Drugs and Poisons, London: Pharmaceutical Press, 2011.

    Google Scholar 

  3. Kaale, E., Nyamweru, B.C., Manyanga, V., Chambuso, M., and Layloff, T., Int. J. Chem. Anal. Sci., 2013, vol. 4, no. 2, p. 73.

    Article  CAS  Google Scholar 

  4. Scheiman, J.M., Yeomans, N.D., Talley, N.J., Vakil, N., Chan, F.K., Tulassay, Z., Rainoldi, J.L., Szczepanski, L., Ung, K.A., and Kleczkowski, D., Am. J. Gastroenterol., 2006, vol. 101, no.10, p. 701.

    Article  CAS  PubMed  Google Scholar 

  5. Sena, M.M., Chaudhry, Z.F., Collins, C.H., and Poppi, R.J., J. Pharm. Biomed. Anal., 2004, vol. 36, no. 4, p. 743.

    Article  CAS  PubMed  Google Scholar 

  6. Goh, J.Z., Chiong, H.S., Zuraini, A., Arifah, A., Zakaria, Z.A., Teh, L.K., Salleh, M., and Hakim, M.N., J. Drug Delivery Sci. Technol., 2014, vol. 24, no. 4, p. 361.

    Article  CAS  Google Scholar 

  7. Idowu, S., Adegoke, O.A., Oderinu, B.A., and Olaniyi, A.A., Pak. J. Pharm. Sci., 2006, vol. 19, no. 2, p. 141.

    CAS  PubMed  Google Scholar 

  8. Rashid, Q.N., Bakir, M.H., and Baban, S.O., Tikrit J. Pure Sci., 2016, vol. 21, no. 3, p. 76.

    Google Scholar 

  9. Sengar, M., Gandhi, S., Patil, U., and Rajmane, V., Asian J. Pharm. Clin. Res., 2010, vol. 3, no. 2, p. 89.

    CAS  Google Scholar 

  10. Sane, R.T., Samant, R.S., and Nayak, V.C., Drug Dev. Ind. Pharm., 1987, vol. 13, no. 7, p. 1307.

    Article  CAS  Google Scholar 

  11. EL-Sayed, Y.M., Abdel-Hameed, M.E., Suleiman, M.S., and Najib, N.M., J. Pharm. Pharmacol., 1988, vol. 40, no. 10, p. 727.

  12. Gowramma, B., Rajan, S., Muralidharan, S., Meyyanathan, S., and Suresh, B., Int. J. ChemTech. Res., 2010, vol. 2, no. 1, p. 676.

    CAS  Google Scholar 

  13. Lee, H.S., Jeong, C.K., Choi, S.J., Kim, S.B., Lee, M.H., Ko, G.I., and Sohn, D.H., J. Pharm. Biomed. Anal., 2000, vol. 23, no. 5, p. 775.

    Article  CAS  PubMed  Google Scholar 

  14. Elkady, E.F., Talanta, 2010, vol. 82, no. 4, p. 1604.

    Article  CAS  PubMed  Google Scholar 

  15. Orlandini, S., Pasquini, B., Caprini, C., Del Bubba, M., Squarcialupi, L., Colotta, V., and Furlanetto, S., J. Chromatogr. A, 2016, vol. 1466, p. 189.

    Article  CAS  PubMed  Google Scholar 

  16. Belal, T., Bedair, M., Gazy, A., and Guirguis, K., Acta Chromatogr., 2015, vol. 27, no. 3, p. 477.

    Article  CAS  Google Scholar 

  17. Shaalan, R.A. and Belal, T.S., Sci. Pharm., 2013, vol. 81, no. 3, p. 713.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Shah, I., Barker, J., Naughton, D.P., Barton, S.J., and Ashraf, S.S., Chem. Cent. J., 2016, vol. 10, no. 1, p. 52.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Dighe, V., Sane, R., Menon, S., Tambe, H., Pillai, S., and Gokarn, V., J. Planar Chromatogr.—Mod. TLC, 2006, vol. 19, no. 112, p. 443.

    Article  CAS  Google Scholar 

  20. Dhaneshwar, S.R. and Bhusari, V.K., Chem. Sin., 2010, vol. 1, no. 2, p. 110.

    CAS  Google Scholar 

  21. Naveed, S. and Qamar, F., Global J. Pharm. Res., 2014, vol. 3, no. 2, p. 1921.

    Google Scholar 

  22. Rahman, N., Bano, Z., and Azmi, S.N.H., J. Chin. Chem. Soc., 2008, vol. 55, no. 3, p. 557.

    Article  CAS  Google Scholar 

  23. Sharma, M. and Sharma, S., J. Pharm. Technol. Drug Res., 2011, vol. 3, p. 1186.

    CAS  Google Scholar 

  24. Jain, M.S., Agrawal, Y.S., Chavhan, R.B., Bari, M.M., and Barhate, S., Asian J. Pharm. Anal., 2012, vol. 2, no. 4, p. 106.

    Google Scholar 

  25. Prabu, S.L., Shirwaikar, A., Shirwaikar, A., Kumar, C.D., Joseph, A., and Kumar, R., Indian J Pharm. Sci., 2008, vol. 70, no. 1, p. 128.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Belal, F., Sharaf, E.D., Tolba, M.M., and Alaa, H., Luminescence, 2015, vol. 30, no. 3, p. 343.

    Article  CAS  PubMed  Google Scholar 

  27. El-Kimary, E. and Ragab, M., J. Planar Chromatogr.—Mod. TLC, 2015, vol. 28, no. 1, p. 74.

    Article  CAS  Google Scholar 

  28. Patel, R., Kakadiya, J., Patel, P., and Shah, N., World J. Pharm. Sci., 2014, vol. 3, no. 5, p. 1621.

    CAS  Google Scholar 

  29. Roosewelt, C., Magesh, A., Rekha, A.S., Pandian, P.S., and Gunasekaran, V., Asian J. Chem., 2007, vol. 19, no. 4, p. 2955.

    CAS  Google Scholar 

  30. Raman, N.V., Reddy, K.R., Prasad, A.V., and Ramakrishna, K., Chromatographia, 2008, vol. 68, nos. 7–8, p. 675.

    Article  CAS  Google Scholar 

  31. Pathan, S., Agarwal, N., and Sultana, Y., Int. J. Pharm. Chem. Sci., 2013, vol. 2, no. 1, p. 500.

    Google Scholar 

  32. Nalwade, S.U., Reddy, V.R., Rao, D.D., and Kumar Morisetti, N., J. Pharm. Biomed. Anal., 2012, vol. 57, p. 109.

    Article  CAS  PubMed  Google Scholar 

  33. Zanitti, L., Ferretti, R., Gallinella, B., La Torre, F., Sanna, M.L., Mosca, A., and Cirilli, R., J. Pharm. Biomed. Anal., 2010, vol. 52, no. 5, p. 665.

    Article  CAS  PubMed  Google Scholar 

  34. Khalil, M.T., Usman, M., Khan, G.M., Awan, S.B., Bibi, H., and Siddiqua, A., Int. J. Drug Dev. Res., 2012, vol. 4, no. 4, p. 252.

    CAS  Google Scholar 

  35. Onal, A. and Oztunc, A., J. Food Drug Anal., 2006, vol. 14, no. 1, p. 12.

    CAS  Google Scholar 

  36. Jain, D.K., Jain, N., and Charde, R., Pharm. Methods, 2011, vol. 2, no. 3, p. 167.

    Article  PubMed  PubMed Central  Google Scholar 

  37. Razzaq, S.N., Ashfaq, M., Khan, I.U., and Mariam, I., J. Chil. Chem. Soc., 2012, vol. 57, no. 4, p. 1456.

    Article  CAS  Google Scholar 

  38. Reddy, P.S., Sait, S., Vasudevmurthy, G., Vishwanath, B., Prasad, V., and Reddy, S.J., Pharma Chem., 2011, vol. 3, no. 6, p. 553.

    CAS  Google Scholar 

  39. Gohil, D., Bhamre, P., Jain, R., and Rajput, S., Int. J. Pharm. Sci. Res., 2014, vol. 5, no. 3, p. 907.

    Google Scholar 

  40. Patel, B., Suhagia, B., Patel, M., and Patel, J., Chromatographia, 2007, vol. 65, nos. 11–12, p. 743.

    Article  CAS  Google Scholar 

  41. Rao, M.N., Krishna, K., and Babu, B.H., J. Appl. Pharm. Sci., 2016, vol. 6, no. 2, p. 072.

  42. AL-Sharqawi, A.R., Method development and validation by high performance liquid chromatography for simultaneous determination of Esomeprazole and Tadalafil in pharmaceutical formulation, M.S. Thesis, Amman: Univ. Petra, 2013.

  43. Malisetty, S.K. and Rambabu, C., Pharm. Methods, 2013, vol. 4, no. 1, p. 26.

    Article  Google Scholar 

  44. Gohil, D. and Rajput, S., Int. J. Pharm. Sci. Res., 2013, vol. 4, no. 2, p. 2435.

    Google Scholar 

  45. Khan, F., Ahmad, I., Akhtar, M., Rauf, H.A., Altaf, H., and Hayat, M.M., Pharm. Chem. J., 2016, vol. 49, no. 11, p. 788.

    Article  CAS  Google Scholar 

  46. Kaura, A.K., Gupta, V., Kaura, M., Roy, G., and Bansal, P., J. Pharm. Res., 2013, vol. 7, no. 5, p. 404.

    CAS  Google Scholar 

  47. Fernandes, N., Nimdeo, M.S., Choudhari, V.P., Kulkarni, R.R., Pande, V.V., and Nikalje, A.G., Int. J. Chem. Sci., 2008, vol. 6, no. 1, p. 29.

    CAS  Google Scholar 

  48. Baghdady, Y.Z., Al-Ghobashy, M.A., Abdel Aleem, A.E., and Weshahy, S.A., J. Adv. Res., 2013, vol. 4, no. 6, p. 51.

    Article  CAS  PubMed  Google Scholar 

  49. Mabrouk, M., El-Fatatry, H., Hammad, S., and Wahbi, A.A.M., J. Pharm. Biomed. Anal., 2003, vol. 33, no. 4, p. 597.

    Article  CAS  PubMed  Google Scholar 

  50. International Conference on Harmonization (ICH) Guidelines Topic Q2(R1): Validation of Analytical Procedures: Text and Methodology, London: European Medicines Agency, 1995.

  51. International Conference on Harmonization (ICH) Guidelines Q2B: Validation of Analytical Procedures: Methodology, Geneva: International conference on harmonization of technical requirements for registration of pharmaceuticals for human use, 1996.

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Authors and Affiliations

  1. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, El Guish Street, 31512, Tanta, Egypt

    Mokhtar M. Mabrouk, Sherin F. Hammad, Fotouh R. Mansour & Basma Z. El-Khateeb

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  1. Mokhtar M. Mabrouk
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  2. Sherin F. Hammad
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Correspondence to Basma Z. El-Khateeb.

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Mokhtar M. Mabrouk, Hammad, S.F., Mansour, F.R. et al. Simultaneous Determination of Diclofenac and Esomeprazole by Reversed phase Liquid Chromatography, Dual Wavelength and Derivative Spectrophotometry. J Anal Chem 74, 458–466 (2019). https://doi.org/10.1134/S1061934819050071

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