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Electric birefringence study of an amyloid fibril system: The short end of the length distribution

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Abstract.

In this article, a system of amyloid fibrils, based on the protein β-lactoglobulin, is studied by transient electric birefringence. Single pulses of an electric field were applied to the solution, and the initial rise and subsequent decay of birefringence analysed. The decay takes place on a range of relaxation times, and therefore contains information about the length distribution of fibrils in the system. The information can be extracted using theories of the electric polarisability of polyelectrolyte rods, since the fibrils are an example of these. Despite the long-standing complications of such theories, useful quantitative information about the system can still be obtained. Using the Fixman model of polyelectrolyte polarisability, we obtain a measurement of the short end of the length distribution which shows the fibril concentration as a function of length rising linearly from 0.02-2 μm. The short end of the length distribution was unobtainable in our previous study using rheo-optics (S.S. Rogers et al., Macromolecules 38, 2948 (2005)), but reasonable agreement between the two techniques shows they are complementary.

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Authors and Affiliations

  1. Department of Physics, Cambridge University, CB3 0HE, Cambridge, UK

    S. S. Rogers & A. M. Donald

  2. Laboratory of Food Physics, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands

    P. Venema, L. M. C. Sagis & E. van der Linden

  3. Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands

    J. P. M. van der Ploeg

Authors
  1. S. S. Rogers
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  2. P. Venema
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  3. J. P. M. van der Ploeg
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  4. L. M. C. Sagis
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  5. A. M. Donald
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  6. E. van der Linden
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Rogers, S.S., Venema, P., van der Ploeg, J.P.M. et al. Electric birefringence study of an amyloid fibril system: The short end of the length distribution. Eur. Phys. J. E 18, 207–217 (2005). https://doi.org/10.1140/epje/i2005-10038-2

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  • DOI: https://doi.org/10.1140/epje/i2005-10038-2

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