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Dynamic light scattering and 31P NMR study of the self-assembly of deoxyguanosine 5’-monophosphate: the effect of added salt

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Abstract.

Self-assembling and dynamical properties of deoxyguanosine 5’-monophosphate in isotropic aqueous solutions were studied by 31P NMR spectroscopy and dynamic light scattering (DLS). All solutions had the same c = 4 wt% guanosine concentration, while the added KCl molarity ranged from 0 to 1.5 M. 31P NMR measurements show that potassium ions strongly enhance the stacking process of guanosine tetramers until a saturation is reached at 0.1 M KCl with more than 70% of the molecules aggregated. Polarized light scattering reveals the presence of a fast relaxation mode that arises from the translational dynamics of the self-assembled stacks. The diffusion coefficient of this mode shows a strong dependence on molarity of added salt, which can be described in terms of the coupled mode and counterion condensation theories for polyelectrolyte solutions. Depolarized light scattering reveals the rotational dynamics of the self-assembled stacks which exhibits a pronounced slowing down with increasing the added salt content.

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Authors and Affiliations

  1. Faculty of Mechanical Engineering, University of Maribor, Smetanova 17, 2000, Maribor, Slovenia

    L. Spindler

  2. Faculty of Mathematics and Physics, University of Ljubljana, Jadranska 19, 1000, Ljubljana, Slovenia

    I. Drevenšek-Olenik & M. Čopič

  3. Jozef Stefan Institute, Jamova 39, 1000, Ljubljana, Slovenia

    I. Drevenšek-Olenik & M. Čopič

  4. Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000, Ljubljana, Slovenia

    J. Cerar, J. Škerjanc & P. Mariani

Authors
  1. L. Spindler
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  2. I. Drevenšek-Olenik
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  3. M. Čopič
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  4. J. Cerar
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  5. J. Škerjanc
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  6. P. Mariani
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Corresponding author

Correspondence to L. Spindler.

Additional information

Received: 19 November 2003, Published online: 2 March 2004

PACS:

81.16.Dn Self-assembly - 61.30.St Lyotropic phases - 82.35.Rs Polyelectrolytes

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Spindler, L., Drevenšek-Olenik, I., Čopič, M. et al. Dynamic light scattering and 31P NMR study of the self-assembly of deoxyguanosine 5’-monophosphate: the effect of added salt. Eur. Phys. J. E 13, 27–33 (2004). https://doi.org/10.1140/epje/e2004-00037-0

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  • DOI: https://doi.org/10.1140/epje/e2004-00037-0

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