Skip to main content
SpringerLink
Log in

Analysis of mitochondrial DNA heteroplasmy of fertile and male-sterile sugar beet plants (Beta vulgaris)

  • Published:
Russian Journal of Genetics: Applied Research

Abstract

Highly sensitive real time PCR has been applied for the first time for the estimation of the heteroplasmy state of plant mtDNA using hydrolyzing fluorescent oligonucleotides probes. The copy numbers of the universal and N and Svulg specific markers in mtDNA of the N and Svulg sugar beet types are estimated using optimized real time PCR for an enlarged sample of plants of various lines and varieties; it was shown for the first time that heteroplasmy is a natural state of Beta vulgaris mtDNA of both the N and Svulg types. The quantity of the dominant and minor variants can differ by more than six orders of magnitude.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arrieta-Montiel, M., Lyznik, A., Woloszynska, M., et al., Tracing Evolutionary and Developmental Implications of Mitochondrial Stoichiometric Shifting in the Common Bean, Genetics, 2001, vol. 158, pp. 851–864.

    PubMed  CAS  Google Scholar 

  • Aul’chenko, Yu.S., Veprev, S.G., and Aksenovich, T.I., Conversion of Cytoplasm Type in Sugar Beet (Beta vulgaris L.) during Inbreeding: Segregation Pedigree Analysis, Russ. J. Genet., 1997, vol. 33, no. 7, pp. 797–802.

    Google Scholar 

  • Bragin, A.G., Ivanov, M.K., and Dymshits, G.M., Estimation of Stoichiometry of Marker Sequences of the Sugar Beet (Beta vulgaris) Mitochondrial Genome by RealTime PCR, Dokl. Biol. Sci., 2006, vol. 406, no. 2, pp. 47–51.

    Article  PubMed  CAS  Google Scholar 

  • Brears, T. and Lonsdale, D.M., The Sugarbeet Mt Genome: A Complex Organization Generated by Homologous Recombination, Mol. Gen. Genet., 1988, vol. 214, pp. 514–522.

    Article  CAS  Google Scholar 

  • Cheng, D., Kitazaki, K., Xu, D., et al., The Distribution of Normal and Male-Sterile Cytoplasms in Chinese Sugar-Beet Germplasm, Euphytica, 2009, vol. 165, pp. 345–351.

    Article  Google Scholar 

  • Kmiec, B., Woloszynska, M., and Janska, H., Heteroplasmy as a Common State of Mitochondrial Genetic Information in Plants and Animals, Curr. Genet., 2006, vol. 50, pp. 149–159.

    Article  PubMed  CAS  Google Scholar 

  • Kubo, T., Nishizawa, S., Sugawara, A., et al., The Complete Nucleotide Sequence of the Mitochondrial Genome of Sugar Beet (Beta vulgaris L.) Reveals a Novel Gene for tRNACys(GCA), Nucleic Acids Res., 2000, vol. 28, pp. 2571–2576.

    Article  PubMed  CAS  Google Scholar 

  • Kubo, T. and Mikami, T., Organization and Variation of Angiosperm Mitochondrial Genome, Physiol. Plant., 2007, vol. 129, pp. 6–13.

    Article  CAS  Google Scholar 

  • Mglinets, A.V., Veprev, S.G., and Maletskii, S.I., Principles and Methods for Creating Analogs of cis-Inbred Lines of Sugar Beet (Beta vulgaris L.). Catalogue of the Collection, in Geneticheskie kollektsii rastenii (Genetic Collections of Plants), Novosibirsk: Inst. Tsitol. Genet. SO RAN, 1995, vol. 3, pp. 229–249.

    Google Scholar 

  • Rogstad, S.H., Plant DNA Extraction Using Silica, Plant Mol. Biol. Reporter, 2003, p. 463.

  • Rybalkin, I.N., Mukhamedova, N.M., Vlasik, T.N., and Chenchik, A.A., The New Technology of Hot Start Polymerase Chain Reaction, Bull. Exptl. Biol. Med., 1996, vol. 121, no. 2, pp. 0007–4888.

    Google Scholar 

  • Sarria, R., Lyznik, A., Vallejos, C.E., and Mackenzie, S.A., A Cytoplasmic Male Sterility-Associated Mitochondrial Peptide Is Post-Translationally Regulated, Plant Cell, 1998, no. 10, pp. 1217–1228.

  • Satoh, M., Kubo, T., Nishizawa, S., et al., The Cytoplasmic Male-Sterile Type and Normal Type Mitochondrial Genomes of Sugar Beet Share the Same Complement of Genes of Known Function but Differ in the Content of Expressed ORFs, Mol. Gen. Genomics, 2004, vol. 272, pp. 247–256.

    Article  CAS  Google Scholar 

  • Schnable, P.S. and Wise, R.P., The Molecular Basis of Cytoplasmic Male Sterility and Fertility Restoration, Trends Plant Sci., 1998, vol. 3, no. 5, pp. 175–180.

    Article  Google Scholar 

  • Veprev, S.G., Dikalova, A.E., Mglinets, A.V., et al., Conversion of Cytoplasm Type in Sugar Beet (Beta vulgaris L.) during Inbreeding: Genetic Analysis and Identification of Mitochondrial DNA Type, Russ. J. Genet., 1997, vol. 33, no. 7, pp. 789–796.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    A. G. Bragin, M. K. Ivanov, L. A. Fedoseeva & G. M. Dymshits

  2. Novosibirsk State University, Novosibirsk, Russia

    M. K. Ivanov & G. M. Dymshits

Authors
  1. A. G. Bragin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. K. Ivanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. A. Fedoseeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. M. Dymshits
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. G. Bragin.

Additional information

Original Russian Text © A.G. Bragin, M.K. Ivanov, L.A. Fedoseeva, G.M. Dymshits, 2011, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2011, Vol. 15, No. 3, pp. 524–530.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bragin, A.G., Ivanov, M.K., Fedoseeva, L.A. et al. Analysis of mitochondrial DNA heteroplasmy of fertile and male-sterile sugar beet plants (Beta vulgaris). Russ J Genet Appl Res 2, 53–57 (2012). https://doi.org/10.1134/S2079059712010030

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S2079059712010030

Keywords

Search

Navigation