Abstract
A modified method for a single run separation and identification of the molecular species of different phospholipid classes in a complex extract has been proposed. It includes reverse phase HPLC with a mass spectrometric detection. This approach has been employed for the analysis of glycerophospholipids and sphingolipids of human erythrocytes and several ceramide fractions have been identified[L2], that were missed in previous studies employing similar methods. The proposed scheme of experiment decreased the number of procedures needed for a complete phospholipid profiling of the sample.
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Original Russian Text © D.A. Korzhenevskiy, A.A. Selischeva, S.V. Saveliev, 2010, published in Biomeditsinskaya Khimiya.
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Korzhenevskiy, D.A., Selischeva, A.A. & Saveliev, S.V. An approach to the identification of molecular fractions of human erythrocyte phospholipids using HPLC with mass-spectrometric detection. Biochem. Moscow Suppl. Ser. B 4, 296–302 (2010). https://doi.org/10.1134/S1990750810030121
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DOI: https://doi.org/10.1134/S1990750810030121