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The role of slow potassium current in phenomena of voltage-dependent action of 4-aminopyridine in amphibian myelinated nerve fibres

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

The mechanism underlying the voltage-dependent action of 4-aminopyridine (4-AP) is investigated in experiments on amphibian myelinated nerve fibres (Rana ridibunda Pallas) by way of extracellular recording of electrical activity and using activators of potassium current (potassium-free solution and nitric oxide NO) and inhibitors of sodium current (tetrodotoxin). Measurement of action potential (AP) areas was used to evaluate the extent of general membrane depolarization during the activity of nerve fibres. Tetrodotoxin-induced decrease in general membrane depolarization (when the action potential amplitude was reduced by less than 20%) leads to an increase in the duration of depolarizing after-potential (DAP). This supports the dependence of time course of DAP in the presence of 4-AP on ratio of fast and slow potassium channels. In the absence of 4-AP, potassium-free solution and NO increase the potassium current through fast potassium channels (decreasing AP duration, reducing DAP and sometimes producing fast hyperpolarizing after-potential (HAP) after shortened AP), and in the presence of 4-AP these activators increase potassium current through unblocked slow potassium channels (making the development of slow HAP induced by 4-AP more rapid). The increase of slow HAP induced by 4-AP under the influence of potassium-free solution with NO supports the idea that slow HAP is due to activation of slow potassium channels and argues against the notion of removal of block of fast potassium channels. All analyzed phenomena of voltage-dependent action of 4-AP in amphibian myelinated nerve fibers can be accounted for by the activation of slow potassium current produced by membrane depolarization and a decrease of the amount of fast potassium channels involved in the membrane repolarization.

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References

  1. Yeh, J.Z., Oxford, G.S., Wu, C.H., and Narahashi, T., Interaction of Aminopyridine with Potassium Channels of Squid Axon Membranes, Biophys. J., 1976, vol. 16, pp. 77–81.

    Article  PubMed  CAS  Google Scholar 

  2. Yeh, J.Z., Oxford, G.S., Wu, C.H., and Narahashi, T., Dynamics of Aminopyridine Block of Potassium Channels in Squid Axon Membranes, J. Gen. Physiol., 1976, vol. 68, pp. 519–535.

    Article  PubMed  CAS  Google Scholar 

  3. Kirsch, G.E., Yeh, J.Z., and Oxford, G.S., Modulation of Aminopyridine Block of Potassium Currents in Squid Axon, Biophys. J., 1986, vol. 50, pp. 637–644.

    Article  PubMed  CAS  Google Scholar 

  4. Hermann, A. and Gorman, A.L.F., Effects of 4-Aminopyridine on Potassium Currents in a Molluscan Neuron, J. Gen. Physiol., 1981, vol. 78, pp. 63–86.

    Article  PubMed  CAS  Google Scholar 

  5. Thompson, S., Aminopyridine Block of Transient Potassium Current, J. Gen. Physiol., 1982, vol. 80, pp. 1–18.

    Article  PubMed  CAS  Google Scholar 

  6. Ulbricht, W. and Wagner, H.-H., Block of Potassium Channels of the Nodal Membrane by 4-Aminopyridine and Its Partial Removal on Depolarization, Pflugers Archiv., 1976, vol. 367, pp. 77–87.

    Article  PubMed  CAS  Google Scholar 

  7. Ulbricht, W., Wagner, H.-H., and Schmidtmayer, J., Effects of Aminopyridines on Potassium Currents of the Nodal Membrane, Advances in the Biosciences, vol. 35, Aminopyridines and Similarly Acting Drugs: Effects on Nerves, Muscles and Synapses, Lechat, P., Thesleff, S., and Bowman, W., Eds, Pergamon Press, Oxford-New York-Toronto-Sydney-Paris-Frankfurt, 1982, pp. 29–41.

    Google Scholar 

  8. Meves, H. and Pichon, Y., The Effect of Internal and External 4-Aminopyridine on the Potassium Currents in Intracellularly Perfused Squid Giant Axons, J. Physiol., 1977, vol. 268, pp. 511–532.

    PubMed  CAS  Google Scholar 

  9. Arhem, P. and Johansson, S., A Model for the Fast 4-Aminopyridine Effects on Amphibian Myelinated Nerve Fibres. A Study Based on Voltage-Clamp Experiments, Acta Physiol. Scand., 1989, vol. 137, pp. 53–61.

    Article  PubMed  CAS  Google Scholar 

  10. Armstrong, C.M. and Loboda, A., A Model of 4-Aminopyridine Action on K Channels: Similarities to Tetraethylammonium Ion, Biophys. J., 2001, vol. 81, pp. 895–904.

    Article  PubMed  CAS  Google Scholar 

  11. Dubois, J.M., Evidence for Existence of Three Types of Potassium Channels in the Frog Node Membrane, J. Physiol., 1981, vol. 318, pp. 279–295.

    PubMed  CAS  Google Scholar 

  12. Ilyin, V.I., Katina, I.E., Lonskii, A.V., Makovsky, V.S., and Polishchuk, E.V., The Cole-Moore Effect in Nodal Membrane of the Frog Rana ridibunda: Evidence for Fast and Slow Potassium Channels, J. Membrane Biol., 1980, vol. 57, pp. 179–193.

    Article  CAS  Google Scholar 

  13. Kuznetzova, I.V., Evstigneev, D.A., and Gluhova, N.V., Transformation of Electrical Activity in the Myelinated Nerve Fibres of Amphibian by 4-Aminopyridine, Fiziologicheskii Zhurnal (Kiev) (Rus.), 2005, vol. 51, no. 2, pp. 96–103.

    CAS  Google Scholar 

  14. Kocsis, J.D., Ruiz, J.A., and Waxman, S.G., Maturation of Mammalian Myelinated Fibers: Changes in Action-Potential Characteristics Following 4-Aminopiridine Application, J. Neurophysiol., 1983, vol. 50, no. 2, pp. 449–463.

    PubMed  CAS  Google Scholar 

  15. Kuznetzova, I.V., Evstigneev, D.A., and Gluhova, N.V., Prolonged After-Potentials of Myelinated Nervous Fibres of Amphibian after Blockage of Potassium Channels by 4-Aminopyridine, Fiziologicheskii Zhurnal (Kiev) (Rus.), 2007, vol. 53, no. 3, pp. 61–69.

    CAS  Google Scholar 

  16. Kuznetzova, I.V., Evstigneev, D.A., and Gluhova, N.V., Some Characteristics of Electrical Activity of Amphibian Myelinated Nervous Fibres Influenced by Potassium and Sodium Channels Blockers, Fundamentalnye Issledovaniya (Rus.), 2007, no. 8, pp. 56–57.

  17. Zefirov, A.L., Katalymov, L.L., Usmendeeva (Kuznetzova), I.V., and Khaliullina, R.R., Effect of Exogenous Nitric Oxide on Electrogenesis in Myelinated and Unmyelinated Nerve Fibers, Bull. Exp. Biol. Med. (Rus.), 2001, vol. 131, no. 5, pp. 604–607.

    Google Scholar 

  18. Kuznetzova, I.V., Evstigneev, D.A., and Gluhova, N.V., Exogenous Nitric Oxide Activate Potassium Channels of Amphibian Myelinated Nervous Fibres, Fundamentalnye Issledovaniya (Rus.), 2007, no. 8, pp. 57–58.

  19. Kuznetzova, I.V., Evstigneev, D.A., Gluhova, N.V., and Gluhov, V.P., Exogenous Nitric Oxide (NO) Action on Long Afterpotentials of Myelinated Nerve Fibers of Amphibians, Fundamentalnye Issledovaniya (Rus.), 2008, no. 1, pp. 17–25.

  20. Dubois, J.M., Properties and Physiological Roles of K+ Currents in Frog Myelinated Nerve Fibres as Revealed by 4-Amino Pyridine, Advances in the Biosciences., vol. 35, Aminopyridines and Similarly Acting Drugs: Effects on Nerves, Muscles and Synapses, Lechat, P., Thesleff, S., and Bowman, W., Eds, Pergamon Press, Oxford-New York-Toronto-Sydney-Paris-Frankfurt, 1982, pp. 43–51.

    Google Scholar 

  21. Dubois, J.M., Potassium Current in the Frog Node of Ranvier, Prog. Biophys. Molec. Biol., 1983, vol. 42, pp. 1–20.

    Article  CAS  Google Scholar 

  22. Vergun, O.V., Kroncheva, S.N., and Valkina, O.N., 4-Aminopyridine Increases After-Potentials and Induces the Spontaneous Activity in Single Myelinated Nerve Fibres, Biologicheskie Membrany (Rus.), 1995, vol. 12, no. 1, pp. 9–72. [Transl. version in Membr. Cell Biol., 1995, vol. 9, pp. 69–74].

    Google Scholar 

  23. Poulter, M.O. and Padjen, A.L., Different Voltage-Dependent Potassium Conductances Regulate Action Potential Repolarization in Frog Myelinated Axon, Neuroscience, 1995, vol. 68, no. 2, pp. 497–504.

    Article  PubMed  CAS  Google Scholar 

  24. Kocsis, J.D., Eng, D.L., Gordon, T.R., and Waxman, S.G., Functional Differences between 4-Aminopyridine and Tetraethylammonium-Sensitive Potassium Channels in Myelinated Axons, Neuroscience Lett., 1987, vol. 75, pp. 193–198.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. I. N. Ul’yanov State Pedagogical University of Ul’yanovsk, pl. 100-letiya so dnia rozhdenia V. I. Lenina, 4, Ul’yanovsk, 432700, Russia

    I. V. Kuznetzova & N. V. Glukhova

  2. Ul’yanovsk Higher Civil Aviation School, ul. Mozhaiskogo, 8, Ul’yanovsk, 432071, Russia

    D. A. Evstigneev

Authors
  1. I. V. Kuznetzova
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  2. D. A. Evstigneev
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  3. N. V. Glukhova
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Correspondence to I. V. Kuznetzova.

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Original Russian Text © I.V. Kuznetzova, D.A. Evstigneev, N.V. Glukhova, 2009, published in Biologicheskie Membrany, 2009, vol. 26, No. 3, pp. 206–216.

The article was translated by the authors.

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Kuznetzova, I.V., Evstigneev, D.A. & Glukhova, N.V. The role of slow potassium current in phenomena of voltage-dependent action of 4-aminopyridine in amphibian myelinated nerve fibres. Biochem. Moscow Suppl. Ser. A 3, 196–205 (2009). https://doi.org/10.1134/S1990747809020135

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  • DOI: https://doi.org/10.1134/S1990747809020135

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