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The cole-moore effect in nodal membrane of the frogRana ridibunda: Evidence for fast and slow potassium channels

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Summary

The K conductance (g K) kinetics were studied in voltage-clamped frog nodes (Rana ridibunda) in double-pulse experiments. The Cole-Moore translation forg K−t curves associated with different initial potentials (E) was only observed with a small percentage of fibers. The absence of the translation was found to be caused by the involvement of an additional, slow,g K component. This component cannot be attributed to a multiple-state performance of the K channel. It can only be accounted for by a separate, slow K channel, the fast channel being the same as then 4 K channel inR. pipiens.

The slow K channel is characterized by weaker sensitivity to TEA, smaller density, weaker potential (E) dependence, and somewhat more negativeE range of activation than the fast K channel. According to characteristics of the slow K system, three types of fibers were found. In Type I fibers (most numerous) the slow K channel behaves as ann 4 HH channel. In Type II fibers (the second largest group found) the slow K channel obeys the HH kinetics within a certainE range only; beyond this range the exponential decline of the slowg K component is preceded by anE-dependent delay, its kinetics after the delay being the same as those in Type I fibers. In Type III fibers (rare) the slow K channel is lacking, and it is only in these fibers that the Cole-Moore translation of the measuredg K−t curves can be observed directly.

The physiological role of the fast and slow K channel in amphibian nerves is briefly discussed.

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Authors and Affiliations

  1. Institute of Physiology, Leningrad University, 199164, Leningrad, USSR

    V. I. Ilyin, Irene E. Katina, A. V. Lonskii, V. S. Makovsky & E. V. Polishchuk

  2. Leningrad Nuclear Physics Institute, Academy of Science USSR, 188350, Gatchina, Leningrad District, USSR

    V. I. Ilyin, Irene E. Katina, A. V. Lonskii, V. S. Makovsky & E. V. Polishchuk

  3. Institute of Cytology, Academy Science USSR, 190121, Leningrad, USSR

    V. I. Ilyin, Irene E. Katina, A. V. Lonskii, V. S. Makovsky & E. V. Polishchuk

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  1. V. I. Ilyin
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  2. Irene E. Katina
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  3. A. V. Lonskii
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  4. V. S. Makovsky
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  5. E. V. Polishchuk
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Ilyin, V.I., Katina, I.E., Lonskii, A.V. et al. The cole-moore effect in nodal membrane of the frogRana ridibunda: Evidence for fast and slow potassium channels. J. Membrain Biol. 57, 179–193 (1980). https://doi.org/10.1007/BF01869586

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