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Ouabain-sensitive and ouabain-resistant isoforms of Na+,K+-ATPase in cerebellar granule cells control MAP kinase activity

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Three-hour incubation of rat cerebellar granule cells with 0.1 μM ouabain increases intracellular levels of Ca2+ ions and reactive oxygen species (ROS) resulting in pronounced activation of Mitogen-Activated Protein Kinase (MAPK). Higher concentrations of ouabain induce further increases in MAPK activity. The activating effect of ouabain is attenuated by the NMDA-receptor antagonists MK-801 and D-AP5. The data obtained suggest that similar to NMDA receptors ouabain-sensitive and ouabain-resistant isoforms of Na+,K+-ATPase are actively involved in intracellular signaling cascades controlling proliferative activity of neuronal cells.

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Authors and Affiliations

  1. Department of Biochemistry, Lomonosov Moscow State University, Moscow, 119991, Russia

    L. V. Karpova, E. E. Akkuratov, E. R. Bulygina & A. A. Boldyrev

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  1. L. V. Karpova
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  2. E. E. Akkuratov
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  3. E. R. Bulygina
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  4. A. A. Boldyrev
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Correspondence to A. A. Boldyrev.

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Original Russian Text © L.V. Karpova, E.E. Akkuratov, E.R. Bulygina, A.A. Boldyrev, 2008, published in Biologicheskie Membrany, 2008, Vol. 25, No. 2, pp. 111–116.

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Karpova, L.V., Akkuratov, E.E., Bulygina, E.R. et al. Ouabain-sensitive and ouabain-resistant isoforms of Na+,K+-ATPase in cerebellar granule cells control MAP kinase activity. Biochem. Moscow Suppl. Ser. A 2, 156–160 (2008). https://doi.org/10.1134/S1990747808020104

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  • DOI: https://doi.org/10.1134/S1990747808020104

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