Abstract
Glutathione peroxidase activity was studied in structures of the white and gray matter of adult female rats using H2O2 as a substrate and Ellman’s reagent to determine the decline in the level of the reduced glutathione. The maximum activity expressed in μmole GSH/min/mg protein was observed in the pyramids of the medulla oblongata (white matter) and the minimum was found in the anterior hypothalamic periventricular area, including the retrochiasmic area, and the medial septal area (mainly composed of gray matter). These differences were significant at p < 0.01 according to Dunn’s Q-test. The medians for each sample were as follows: 1.16 in the pyramids of the medulla oblongata; 1.02 in the optic chiasm; 0.83 in the olfactory tubercle; 0.71 in the corpus callosum; 0.66 in the anterior commissure; 0.42 in the medial septal area; and 0.37 in the anterior hypothalamic periventricular area. Thus, at least in some structures of the white matter, the glutathione peroxidase activity was higher as compared to the structures of the gray matter. These data disprove the hypothesis that the prevalence of the activity of this enzyme occurs in the gray matter.
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Original Russian Text © A.V. Razygraev, 2012, published in Neirokhimiya, 2012, Vol. 29, No. 1, pp. 19–22.
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Razygraev, A.V. Glutathione peroxidase activity in structures of the white and gray matter of the rat brain. Neurochem. J. 6, 14–16 (2012). https://doi.org/10.1134/S1819712411040143
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DOI: https://doi.org/10.1134/S1819712411040143