Abstract
ctive: As a result of the study, branched polysaccharides were isolated from the basidiomycete raw materials of Ganoderma lucidum. It has been established that the isolated fractions contain branched polysaccharides in the form of complexes with melanin. Methods: After purification of polysaccharides by ion-exchange chromatography, two fractions were obtained from basidial raw materials: neutral polysaccharides GW-1 with a yield of 25.71% and anionic polysaccharides GW-2, the yield of which was 5.26%, respectively. The physicochemical properties of the obtained samples were studied by IR and UV spectroscopy. The degree of purity of the obtained fractions of branched polysaccharides was established. Using gas chromatography, one-dimensional (13C NMR, 1H NMR) and two-dimensional (COSY, TOCSY, HSQC, HMBC, NOESY) NMR spectroscopy, the composition and molecular structure of the obtained polysaccharide samples were determined. Results and Discussion: The results showed that the isolated and purified polysaccharides are branched glucans with 1,4,6- and 1,3,6-bonds between glucopyranose units. Pharmacotoxicological studies were carried out on white outbred mice and it was found that the resulting polysaccharides belong to class V, practically non-toxic compounds (LD50³ 2000 mg/kg). Conclusions: Isolated polysaccharides (GW) are promising biologically active components, on the basis of which it is possible to create drugs with hepatoprotective and antitumor activity.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Pharmaco-toxicological studies were carried out on mice, in accordance with the model used. Manipulations with laboratory animals were carried out in accordance with the European Convention for the Protection of Vertebrate Animals Used for Experimental or Other Scientific Purposes [European Directive 2010/63/EU on the protection of animals used for scientific purposes. September 22, 2010. Official Journal of the European Union, L 276/33-L276/79]. The animals had free access to food and water, in vivarium conditions, on a standard diet. No conflict of interest was declared by the authors.
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Khaytmetova, S.B., Turaev, A.S., Khalilova, G.A. et al. Isolation, Physico-Chemical Characteristics, and Acute Toxicity Evaluation of Water-Soluble Polysaccharide from Basidial Raw Material Ganoderma lucidum. Russ J Bioorg Chem 50, 95–105 (2024). https://doi.org/10.1134/S1068162024010011
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DOI: https://doi.org/10.1134/S1068162024010011