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Exogenous IAA and ABA stimulate germination of petunia male gametophyte by activating Ca2+-dependent K+-channels and by modulating the activity of plasmalemma H+-ATPase and actin cytoskeleton

  • Developmental Biology of Plants
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Abstract

To date, the molecular mechanisms underlying the osmoregulation of pollen grains (PGs) related to the maintenance of their water status and allowing pollen tubes (PTs) to regulate concentrations in them of osmolytes and transmembrane water transport remain to be not so far characterized. In the present work, the data on the participation of IAA and ABA in the osmoregulation of germinating in vitro petunia male gametophyte were obtained. It has been established that the growth-stimulating effect of these phytohormones is due to their action on intracellular pH (pHc), the membrane potential of plasmalemma (PM), the activity of PM H+-ATPase, K+-channels in the same membrane and organization of actin cytoskeleton (AC). Two possible targets of the action of these compounds are revealed. These are represented by (1) PM H+-ATPase, electrogenic proton pump responsible for polarization of this membrane, and (2) Ca2+-dependent K+-channels. The findings of the present work suggest that the hormone-induced pHc shift is involved in cascade of the events including the functioning of pH-dependent K+-channels. It was shown that the hormoneinduced hyperpolarization of the PM is a result of stimulation of electrogenic activity of PM H+-ATPase and the hormonal effects are mediated by transient elevation in the level of free Ca2+ in the cytosol and generation of reactive oxygen species (ROS). The results on the role of K+ ions in the control of water-driving forces for transmembrane water transport allowed us to formulate the hypothesis that IAA and ABA stimulate germination of PGs and growth of PTs by activating K+-channels. In addition, the studies performed showed that the AC of male gametophyte is sensitive to the action of exogenous phytohormones, with to more extent to the action of IAA. As judged by the action of latrunculin B (LB) the AC may serve as the determinant of the level of endogenous phytohormones that most likely participate in the regulation of the polar growth of PTs impacting on the pool of F-actin in their apical and subapical zones.

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, Moscow, 127276, Russia

    L. V. Kovaleva, A. S. Voronkov, Yu. V. Minkina, G. V. Timofeeva & I. M. Andreev

  2. Russian State Agrarian University—Timiryazev Moscow Agricultural Academy, ul. Timiryazeva 49, Moscow, 127550, Russia

    E. V. Zakharova

Authors
  1. L. V. Kovaleva
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  2. A. S. Voronkov
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  3. E. V. Zakharova
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  4. Yu. V. Minkina
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  5. G. V. Timofeeva
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  6. I. M. Andreev
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Correspondence to L. V. Kovaleva.

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Published in Russian in Ontogenez, 2016, Vol. 47, No. 3, pp. 138–151.

The text was submitted by the authors in English.

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Kovaleva, L.V., Voronkov, A.S., Zakharova, E.V. et al. Exogenous IAA and ABA stimulate germination of petunia male gametophyte by activating Ca2+-dependent K+-channels and by modulating the activity of plasmalemma H+-ATPase and actin cytoskeleton. Russ J Dev Biol 47, 109–121 (2016). https://doi.org/10.1134/S1062360416030036

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