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Signalling pathways in pollen germination and tube growth

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Summary.

Signalling is an integral component in the establishment and maintenance of cellular identity. In plants, tip-growing cells represent an ideal system to investigate signal transduction mechanisms, and among these, pollen tubes (PTs) are one of the favourite models. Many signalling pathways have been identified during germination and tip growth, namely, Ca2+, calmodulin, phosphoinositides, protein kinases, cyclic AMP, and GTPases. These constitute a large and complex web of signalling networks that intersect at various levels such as the control of vesicle targeting and fusion and the physical state of the actin cytoskeleton. Here we discuss some of the most recent advances made in PT signal transduction cascades and their implications for our future research. For reasons of space, emphasis was given to signalling mechanisms that control PT reorientation, so naturally many other relevant works have not been cited.

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Abbreviations

ABP:

actin-binding protein

AC:

adenylyl cyclase

[Ca2+]c :

cytosolic free calcium

CaM:

calmodulin

CDPK:

Ca2+-dependent protein kinase

IP3:

D-myo-inositol-1,4,5-trisphosphate

MF:

microfilament

MT:

microtubule

PA:

phosphatidic acid

PIP2:

phosphatidylinositol-4,5-bisphosphate

PLC:

phospholipase C

PLD:

phospholipase D

PKH:

pollen kinesin homologue

PT:

pollen tube

References

  • K Ainger D Avossa F Morgan SJ Hill C Barry E Barbarese JH Carson (1993) ArticleTitleTransport and localisation of exogenous myelin basic protein mRNA microinjected into oligodendrocytes J Cell Biol 123 431–441 Occurrence Handle7691830 Occurrence Handle1:CAS:528:DyaK3sXmt1amsb8%3D Occurrence Handle10.1083/jcb.123.2.431

    Article  PubMed  CAS  Google Scholar 

  • RA Anderson IV Boronenkov SD Doughman J Kunz JC Loijens (1999) ArticleTitlePhosphoinositol phosphate kinases, a multifaceted family of signalling enzymes J Biol Chem 274 9907–9910 Occurrence Handle10187762 Occurrence Handle1:CAS:528:DyaK1MXis1GjsL4%3D Occurrence Handle10.1074/jbc.274.15.9907

    Article  PubMed  CAS  Google Scholar 

  • F Baluška J Šamaj P Wojtaszek D Volkmann D Menzel (2003) ArticleTitleCytoskeleton-plasma membrane-cell wall continuum in plants: emerging links revisited Plant Physiol 133 482–491 Occurrence Handle14555777 Occurrence Handle10.1104/pp.103.027250 Occurrence Handle1:CAS:528:DC%2BD3sXosVaqtrs%3D

    Article  PubMed  CAS  Google Scholar 

  • JR Bamburg (1999) ArticleTitleProteins of the ADF/cofilin family: essential regulators of actin dynamics Annu Rev Cell Dev Biol 15 185–230 Occurrence Handle10611961 Occurrence Handle1:CAS:528:DC%2BD3cXhtVSnurw%3D Occurrence Handle10.1146/annurev.cellbio.15.1.185

    Article  PubMed  CAS  Google Scholar 

  • D Bar-Sagi A Hall (2000) ArticleTitleRas and Rho GTPases: a family reunion Cell 103 227–238 Occurrence Handle11057896 Occurrence Handle1:CAS:528:DC%2BD3cXns1Cltrg%3D Occurrence Handle10.1016/S0092-8674(00)00115-X

    Article  PubMed  CAS  Google Scholar 

  • G Cai A Bartalesi C Del Casino A Moscatelli A Tiezzi M Cresti (1993) ArticleTitleThe kinesin-immunoreactive homologue from Nicotiana tabacum pollen tube: biochemical properties and subcellular localization Planta 191 496–506 Occurrence Handle1:CAS:528:DyaK2cXkvVOn Occurrence Handle10.1007/BF00195751

    Article  CAS  Google Scholar 

  • G Cai S Romagnoli A Moscatelli E Ovidi G Gambellini A Tiezzi M Cresti (2000) ArticleTitleIdentification and characterization of novel microtubule-based motor associated with membranous organelles in tobacco pollen tubes Plant Cell 12 1719–1736 Occurrence Handle11006343 Occurrence Handle1:CAS:528:DC%2BD3cXnsVylt78%3D Occurrence Handle10.1105/tpc.12.9.1719

    Article  PubMed  CAS  Google Scholar 

  • L Camacho R Malhó (2003) ArticleTitleEndo-exocytosis in the pollen tube apex is differentially regulated by Ca2+ and GTPases J Exp Bot 54 83–92 Occurrence Handle12456758 Occurrence Handle1:CAS:528:DC%2BD3sXlt1KktA%3D%3D Occurrence Handle10.1093/jxb/54.380.83

    Article  PubMed  CAS  Google Scholar 

  • CY-h Chen AY Cheung H-m Wu (2003) ArticleTitleActin-depolymerizing factor mediates Rac/Rop GTPase-regulated pollen tube growth Plant Cell 15 237–249 Occurrence Handle12509534 Occurrence Handle1:CAS:528:DC%2BD3sXmtFemtA%3D%3D Occurrence Handle10.1105/tpc.007153

    Article  PubMed  CAS  Google Scholar 

  • AY Cheung H-m Wu (2004) ArticleTitleOverexpression of an Arabidopsis formin stimulates supernumerary actin cable formation from pollen tube cell membrane Plant Cell 16 257–269 Occurrence Handle14671023 Occurrence Handle1:CAS:528:DC%2BD2cXosVeksQ%3D%3D Occurrence Handle10.1105/tpc.016550

    Article  PubMed  CAS  Google Scholar 

  • AY Cheung CY-h Chen RH Glaven BHJ de Graaf L Vidali PK Hepler H-m Wu (2002) ArticleTitleRab2 GTPase regulates vesicle trafficking between the endoplasmic reticulum and Golgi bodies and is important to pollen tube growth Plant Cell 14 945–962 Occurrence Handle11971147 Occurrence Handle1:CAS:528:DC%2BD38XjsFWktrY%3D Occurrence Handle10.1105/tpc.000836

    Article  PubMed  CAS  Google Scholar 

  • S Cockcroft MA De Matteis (2001) ArticleTitleInositol lipids as spatial regulators of membrane traffic J Membr Biol 180 187–194 Occurrence Handle11337890 Occurrence Handle1:STN:280:DC%2BD3Mris1Gqsw%3D%3D Occurrence Handle10.1007/s002320010069

    Article  PubMed  CAS  Google Scholar 

  • CJ Cooke CJ Smith TJ Walton RP Newton (1994) ArticleTitleEvidence that cyclic AMP is involved in the hypersensitive response of Medicago sativa to a fungal elicitor Phytochemistry 35 889–895 Occurrence Handle1:CAS:528:DyaK2cXis1Kjtrw%3D Occurrence Handle10.1016/S0031-9422(00)90633-2

    Article  CAS  Google Scholar 

  • O Cremona G Di Paolo MR Wenk A Lüthi WT Kim K Takei L Daniell Y Nemoto SB Shears RA Flavell DA McCormick P De Camilli (1999) ArticleTitleEssential role of phophoinositol metabolism in synaptic vesicle recycling Cell 99 179–188 Occurrence Handle10535736 Occurrence Handle1:CAS:528:DyaK1MXmvFeltbo%3D Occurrence Handle10.1016/S0092-8674(00)81649-9

    Article  PubMed  CAS  Google Scholar 

  • AP Dawson (1997) ArticleTitleCalcium signaling: How do IP3 receptors work? Curr Biol 7 R544–R547 Occurrence Handle9285705 Occurrence Handle1:CAS:528:DyaK2sXmsVSju7Y%3D Occurrence Handle10.1016/S0960-9822(06)00277-6

    Article  PubMed  CAS  Google Scholar 

  • C Del Casino Y Li A Moscatelli M Scali A Tiezzi M Cresti (1993) ArticleTitleDistribution of microtubules during the growth of tobacco pollen tubes Biol Cell 79 125–132 Occurrence Handle10.1016/0248-4900(93)90248-D

    Article  Google Scholar 

  • J Derksen B Knuiman K Hoedemaekers G Anouchka S Bonhomme ES Pierson (2002) ArticleTitleGrowth and cellular organization of Arabidopsis pollen tubes in vitro Sex Plant Reprod 15 133–139 Occurrence Handle10.1007/s00497-002-0149-1

    Article  Google Scholar 

  • S Desrivières FT Cooke H Morales-Johansson PJ Parker MN Hall (2002) ArticleTitleCalmodulin controls organization of the actin cytoskeleton regulation of phosphatidylinositol (4,5)-bisphosphate synthesis in Saccharomyces cerevisiae Biochem J 366 945–951 Occurrence Handle12079494

    PubMed  Google Scholar 

  • DD Fisher S Gilroy JR Cyr (1996) ArticleTitleEvidence for opposing effects of calmodulin on cortical microtubules Plant Physiol 112 1079–1087 Occurrence Handle12226434 Occurrence Handle1:CAS:528:DyaK28XntVWrtbc%3D

    PubMed  CAS  Google Scholar 

  • I Foissner F Grolig G Obermeyer (2002) ArticleTitleReversible protein phosphorylation regulates the dynamic organization of the pollen tube cytoskeleton: effects of calyculin A and okadaic acid Protoplasma 220 1–15 Occurrence Handle12417932 Occurrence Handle1:CAS:528:DC%2BD38XosFegurY%3D Occurrence Handle10.1007/s00709-002-0032-9

    Article  PubMed  CAS  Google Scholar 

  • VE Franklin-Tong BK Drøbak AC Allan PAC Watkins AJ Trewavas (1996) ArticleTitleGrowth of pollen tubes of Papaver rhoeas is regulated by a slow moving calcium wave propagated by inositol triphosphate Plant Cell 8 1305–1321 Occurrence Handle12239415 Occurrence Handle1:CAS:528:DyaK28XltlKjtrs%3D Occurrence Handle10.1105/tpc.8.8.1305

    Article  PubMed  CAS  Google Scholar 

  • Y Fu G Wu Z Yang (2001) ArticleTitleRop GTPase-dependent dynamics of tip-localized F-actin controls tip growth in pollen tubes J Cell Biol 152 1019–1032 Occurrence Handle11238457 Occurrence Handle1:CAS:528:DC%2BD3MXhslGmurs%3D Occurrence Handle10.1083/jcb.152.5.1019

    Article  PubMed  CAS  Google Scholar 

  • T Fujimoto A Miyawaki K Mikoshiba (1995) ArticleTitleInositol 1,4,5-trisphosphate receptor-like protein in plasmalemmal caveolae is linked to actin filaments J Cell Sci 108 7–15 Occurrence Handle7738118 Occurrence Handle1:CAS:528:DyaK2MXjsFegu7w%3D

    PubMed  CAS  Google Scholar 

  • A Geitmann E Parre (2004) ArticleTitleThe local cytomechanical properties of growing pollen tubes correspond to the axial distribution of structural cellular elements Sex Plant Reprod 17 9–16 Occurrence Handle10.1007/s00497-004-0210-3

    Article  Google Scholar 

  • A Geitmann Y-Q Li M Cresti (1995) ArticleTitleThe role of cytoskeleton and dictyosome activity in the pulsatory growth of Nicotiana tabacum and Petunia hybrida pollen tubes Bot Acta 109 102–109

    Google Scholar 

  • BC Gibbon LE Zonia DR Kovar PJ Hussey CJ Staiger (1998) ArticleTitlePollen profilin function depends on interaction with proline-rich motifs Plant Cell 10 981–993 Occurrence Handle9634586 Occurrence Handle1:CAS:528:DyaK1cXktl2gsb0%3D Occurrence Handle10.1105/tpc.10.6.981

    Article  PubMed  CAS  Google Scholar 

  • BL Goode DG Drubin G Barnes (2000) ArticleTitleFunctional cooperation between the microtubule and actin cytoskeletons Curr Opin Cell Biol 12 63–71 Occurrence Handle10679357 Occurrence Handle1:CAS:528:DC%2BD3cXhsVOjtr8%3D Occurrence Handle10.1016/S0955-0674(99)00058-7

    Article  PubMed  CAS  Google Scholar 

  • S Grabski E Arnoys B Busch M Schindler (1998) ArticleTitleRegulation of actin tension in plant cells by kinases and phosphatases Plant Physiol 116 279–290 Occurrence Handle1:CAS:528:DyaK1cXkslGjsw%3D%3D Occurrence Handle10.1104/pp.116.1.279

    Article  CAS  Google Scholar 

  • AC Harmon M Gribskov JF Harper (2000) ArticleTitleCDPKs – a kinase for every Ca2+ signal? Trends Plant Sci 5 154–159 Occurrence Handle10740296 Occurrence Handle1:STN:280:DC%2BD3c3gslWntg%3D%3D Occurrence Handle10.1016/S1360-1385(00)01577-6

    Article  PubMed  CAS  Google Scholar 

  • TL Holdaway-Clarke PK Hepler (2003) ArticleTitleControl of pollen tube growth: role of ion gradients and fluxes New Phytol 159 539–563 Occurrence Handle1:CAS:528:DC%2BD3sXns1ShtbY%3D Occurrence Handle10.1046/j.1469-8137.2003.00847.x

    Article  CAS  Google Scholar 

  • TL Holdaway-Clarke JA Feijó GR Hackett JG Kunkel PK Hepler (1997) ArticleTitlePollen tube growth and the intracellular cytosolic calcium gradient oscillate in phase while extracellular calcium influx is delayed Plant Cell 9 1999–2010 Occurrence Handle12237353 Occurrence Handle1:CAS:528:DyaK2sXnsl2mt7o%3D Occurrence Handle10.1105/tpc.9.11.1999

    Article  PubMed  CAS  Google Scholar 

  • PJ Hussey M Yuan G Calder S Khan CW Lloyd (1998) ArticleTitleMicroinjection of pollen-specific actin-depolymerizing factor, ZmADF1, reorientates F-actin strands in Tradescantia stamen hair cells Plant J 14 353–357 Occurrence Handle1:CAS:528:DyaK1cXjslOlsr8%3D Occurrence Handle10.1046/j.1365-313X.1998.00122.x

    Article  CAS  Google Scholar 

  • U Joos J van Aken U Kristen (1994) ArticleTitleMicrotubules are involved in maintaining the cellular polarity in pollen tubes of Nicotiana sylvetris Protoplasma 179 5–15 Occurrence Handle10.1007/BF01360732

    Article  Google Scholar 

  • A Kachroo ME Nasrallah JB Nasrallah (2002) ArticleTitleSelf-incompatibility, receptor-ligand signaling, and cell–cell communication in plant reproduction Plant Cell 14 S227–S238 Occurrence Handle12045279 Occurrence Handle1:CAS:528:DC%2BD38XksV2hu74%3D

    PubMed  CAS  Google Scholar 

  • B Kost P Spielhofer N-H Chua (1998) ArticleTitleA GFP-mouse talin fusion protein labels plant actin filaments in vivo and visualizes the actin cytoskeleton in growing pollen tubes Plant J 16 393–401 Occurrence Handle9881160 Occurrence Handle1:CAS:528:DyaK1MXhsVOquw%3D%3D Occurrence Handle10.1046/j.1365-313x.1998.00304.x

    Article  PubMed  CAS  Google Scholar 

  • B Kost E Lemichez P Spielhofer Y Hong K Tolias C Carpenter M-H Chua (1999) ArticleTitleRac homologues and compartmentalized phosphatidylinositol 4,5-bisphosphate act in a common pathway to regulate polar pollen tube growth J Cell Biol 145 317–330 Occurrence Handle10209027 Occurrence Handle1:CAS:528:DyaK1MXislSgtL0%3D Occurrence Handle10.1083/jcb.145.2.317

    Article  PubMed  CAS  Google Scholar 

  • SA Lancelle PK Hepler (1991) ArticleTitleAssociation of actin with cortical microtubules revealed by immunogold localization in Nicotiana pollen tubes Protoplasma 165 167–172 Occurrence Handle1:CAS:528:DyaK38XisFaju7w%3D Occurrence Handle10.1007/BF01322287

    Article  CAS  Google Scholar 

  • H Li Y Lin RM Heath MX Zhu Z Yang (1999) ArticleTitleControl of pollen tube tip growth by a Rop GTPase-dependent pathway that leads to tiplocalized calcium influx Plant Cell 11 1731–1742 Occurrence Handle10488239 Occurrence Handle1:CAS:528:DyaK1MXms1ahsr8%3D Occurrence Handle10.1105/tpc.11.9.1731

    Article  PubMed  CAS  Google Scholar 

  • Y Lin Z Yang (1997) ArticleTitleInhibition of pollen tube elongation by microinjected anti-Rop1Ps antibodies suggests a crucial role for Rho-type GTPases in the control of tip growth Plant Cell 9 1647–1659 Occurrence Handle12237397 Occurrence Handle1:CAS:528:DyaK2sXmsFeltLY%3D Occurrence Handle10.1105/tpc.9.9.1647

    Article  PubMed  CAS  Google Scholar 

  • Y Lin Y Wang J-K Zhu Z Yang (1996) ArticleTitleLocalization of a Rho GTPase implies a role in tip growth and movement of the generative cell in pollen tubes Plant Cell 8 293–303 Occurrence Handle12239385 Occurrence Handle1:CAS:528:DyaK28XhtlKqtrY%3D Occurrence Handle10.1105/tpc.8.2.293

    Article  PubMed  CAS  Google Scholar 

  • GQ Liu G Cai C Del Casino A Tiezzi M Cresti (1994) ArticleTitleKinesin-related polypeptide is associated with vesicles from Corylus avellana pollen Cell Motil Cytoskeleton 29 155–166 Occurrence Handle7820865 Occurrence Handle1:CAS:528:DyaK2MXhvVGgs7c%3D Occurrence Handle10.1002/cm.970290207

    Article  PubMed  CAS  Google Scholar 

  • I Lopez RG Anthony SK Maciver C-J Jiang S Khan AG Weeds PJ Hussey (1996) ArticleTitlePollen specific expression of maize genes encoding actin depolymerizing factor-like proteins Proc Natl Acad Sci USA 93 7415–7420 Occurrence Handle8693008 Occurrence Handle1:CAS:528:DyaK28Xkt1Wnsro%3D Occurrence Handle10.1073/pnas.93.14.7415

    Article  PubMed  CAS  Google Scholar 

  • EM Lord LC Sanders (1992) ArticleTitleRoles for the extracellular matrix in plant development and pollination: a special case of cell movement in plants Dev Biol 153 16–28 Occurrence Handle1516746 Occurrence Handle1:STN:280:By2A1cjksVc%3D Occurrence Handle10.1016/0012-1606(92)90088-X

    Article  PubMed  CAS  Google Scholar 

  • L Ma X Xu S Cui D Sun (1999) ArticleTitleThe presence of a heterotrimeric G protein and its role in signal transduction of extracellular calmodulin in pollen germination and tube growth Plant Cell 11 1351–1364 Occurrence Handle10402434 Occurrence Handle1:CAS:528:DyaK1MXkvFertbk%3D Occurrence Handle10.1105/tpc.11.7.1351

    Article  PubMed  CAS  Google Scholar 

  • R Malhó (1998) ArticleTitleThe role of inositol(1,4,5)triphosphate in pollen tube growth and orientation Sex Plant Reprod 11 231–235 Occurrence Handle10.1007/s004970050145

    Article  Google Scholar 

  • R Malhó L Camacho (2004) Signalling the cytoskeleton in pollen tube germination and growth PJ Hussey (Eds) The plant cytoskeleton in cell differentiation and development SeriesTitleAnnual Plant Reviews NumberInSeries10 Blackwell Oxford 240–264

    Google Scholar 

  • R Malhó AJ Trewavas (1996) ArticleTitleLocalized apical increases of cytosolic free calcium control pollen tube orientation Plant Cell 8 1935–1949 Occurrence Handle12239370 Occurrence Handle10.1105/tpc.8.11.1935

    Article  PubMed  Google Scholar 

  • R Malhó ND Read AJ Trewavas MS Pais (1995) ArticleTitleCalcium channel activity during pollen tube growth and reorientation Plant Cell 7 1173–1184 Occurrence Handle12242402 Occurrence Handle10.1105/tpc.7.8.1173

    Article  PubMed  Google Scholar 

  • R Malhó L Camacho A Moutinho (2000) ArticleTitleSignalling pathways in pollen tube growth and reorientation Ann Bot 85 IssueIDSuppl A 59–68 Occurrence Handle10.1006/anbo.1999.0991

    Article  Google Scholar 

  • TFJ Martin (1998) ArticleTitlePhosphoinositide lipids as signaling molecules: common themes for signal transduction, cytoskeletal regulation, and membrane trafficking Annu Rev Cell Dev Biol 14 231–264 Occurrence Handle9891784 Occurrence Handle1:CAS:528:DyaK1cXnvFyju7k%3D Occurrence Handle10.1146/annurev.cellbio.14.1.231

    Article  PubMed  CAS  Google Scholar 

  • J Mathur N Mathur M Hülskamp (2002) ArticleTitleSimultaneous visualization of peroxisomes and cytoskeletal elements reveals actin and not microtubulebased peroxisome motility in plants Plant Physiol 128 1031–1045 Occurrence Handle11891258 Occurrence Handle1:CAS:528:DC%2BD38Xit1GqtL0%3D Occurrence Handle10.1104/pp.011018

    Article  PubMed  CAS  Google Scholar 

  • M Messerli KR Robinson (1997) ArticleTitleTip localized Ca2+ pulses are coincident with peak pulsatile growth rates in pollen tubes of Lilium longiflorum J Cell Sci 110 1269–1278 Occurrence Handle9202387 Occurrence Handle1:CAS:528:DyaK2sXktF2mtb0%3D

    PubMed  CAS  Google Scholar 

  • MA Messerli R Créton LF Jaffe KR Robinson (2000) ArticleTitlePeriodic increases in elongation rate precede increases in cytosolic Ca2+ during pollen tube growth Dev Biol 222 84–98 Occurrence Handle10885748 Occurrence Handle1:CAS:528:DC%2BD3cXksFWmsLY%3D Occurrence Handle10.1006/dbio.2000.9709

    Article  PubMed  CAS  Google Scholar 

  • DD Miller SP Scordilis PK Hepler (1995) ArticleTitleIdentification and localization of three classes of myosins in pollen tubes of Lilium longiflorum and Nicotiana alata J Cell Sci 108 2549–2563 Occurrence Handle7593296 Occurrence Handle1:CAS:528:DyaK2MXmvFOrtrw%3D

    PubMed  CAS  Google Scholar 

  • DD Miller SA Lancelle PK Hepler (1996) ArticleTitleActin filaments do not form a dense meshwork in Lilium longiflorum pollen tube tips Protoplasma 195 123–132 Occurrence Handle10.1007/BF01279191

    Article  Google Scholar 

  • I Mittermann I Swoboda E Pierson N Eller D Kraft R Valenta E Heberle-Bors (1995) ArticleTitleMolecular cloning and characterization of pro- filin from tobacco (Nicotiana tabacum): increased profilin expression during pollen maturation Plant Mol Biol 27 137–146 Occurrence Handle7865783 Occurrence Handle1:CAS:528:DyaK2MXktFWlsb0%3D Occurrence Handle10.1007/BF00019185

    Article  PubMed  CAS  Google Scholar 

  • AJ Molendjik F Bischoff CSV Rajendrakumar J Friml M Braun S Gilroy K Palme (2001) ArticleTitle Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth EMBO J 20 2779–2788 Occurrence Handle10.1093/emboj/20.11.2779

    Article  Google Scholar 

  • D Monteiro Q Liu S Lisboa GEF Scherer H Quader R Malhó (2005) ArticleTitlePhosphoinositides and phosphatidic acid regulate pollen tube growth and reorientation through modulation of [Ca2+]c, and membrane secretion J Exp Bot 56 1665–1674 Occurrence Handle15837704 Occurrence Handle1:CAS:528:DC%2BD2MXktl2qurg%3D Occurrence Handle10.1093/jxb/eri163

    Article  PubMed  CAS  Google Scholar 

  • I Moore T Diefenthal V Zarsky J Shell K Palme (1997) ArticleTitleA homolog of the mammalian GTPase Rab2 is present in Arabidopsis and is expressed predominantly in pollen grains and seedlings Proc Natl Acad Sci USA 94 762–767 Occurrence Handle9012859 Occurrence Handle1:CAS:528:DyaK2sXnvVGrsg%3D%3D Occurrence Handle10.1073/pnas.94.2.762

    Article  PubMed  CAS  Google Scholar 

  • A Moscatelli C Del Casino L Lozzi G Cai M Scali A Tiezzi M Cresti (1995) ArticleTitleHigh molecular weight polypeptides related to dynein heavy chains in Nicotiana tabacum pollen tubes J Cell Sci 108 1117–1125 Occurrence Handle7622598 Occurrence Handle1:CAS:528:DyaK2MXkvFalt78%3D

    PubMed  CAS  Google Scholar 

  • A Moscatelli G Cai F Ciampolini M Cresti (1998) ArticleTitleDynein heavy chainrelated polypeptides are associated with organelles in pollen tubes of Nicotiana tabacum Sex Plant Reprod 11 31–40 Occurrence Handle1:CAS:528:DyaK1cXitF2jsro%3D Occurrence Handle10.1007/s004970050118

    Article  CAS  Google Scholar 

  • A Moscatelli G Cai M Cresti (1999) Actin filament- and microtubule-based motor systems: their concerted action during pollen tube growth M Cresti G Cai A Moscatelli (Eds) Fertilization in higher plants: molecular and cytological aspects Springer Berlin Heidelberg New York 301–315

    Google Scholar 

  • A Moutinho AJ Trewavas R Malhó (1998a) ArticleTitleRelocation of a Ca2+-dependent protein kinase activity during pollen tube reorientation Plant Cell 10 1499–1510 Occurrence Handle1:CAS:528:DyaK1cXmsVKiu7w%3D Occurrence Handle10.1105/tpc.10.9.1499

    Article  CAS  Google Scholar 

  • A Moutinho J Love AJ Trewavas R Malhó (1998b) ArticleTitleDistribution of calmodulin protein and mRNA in growing pollen tubes Sex Plant Reprod 11 131–139 Occurrence Handle1:CAS:528:DyaK1cXlsVSjtr0%3D Occurrence Handle10.1007/s004970050130

    Article  CAS  Google Scholar 

  • A Moutinho PJ Hussey AJ Trewavas R Malhó (2001) ArticleTitlecAMP acts as a second messenger in pollen tube growth and reorientation Proc Natl Acad Sci USA 98 10481–10486 Occurrence Handle11517303 Occurrence Handle1:CAS:528:DC%2BD3MXmvFWhs7s%3D Occurrence Handle10.1073/pnas.171104598

    Article  PubMed  CAS  Google Scholar 

  • T Munnik (2001) ArticleTitlePhosphatidic acid: an emerging plant lipid second messenger Trends Plant Sci 6 227–233 Occurrence Handle11335176 Occurrence Handle1:CAS:528:DC%2BD3MXlsFKmtbs%3D Occurrence Handle10.1016/S1360-1385(01)01918-5

    Article  PubMed  CAS  Google Scholar 

  • N O’Luanaigh R Pardo A Fensome V Allen-Baume D Jones MR Holt S Cockcroft (2002) ArticleTitleContinual production of phosphatidic acid by phospholipase D is essential for antigen-stimulated membrane ruffling in cultured mast cells Mol Biol Cell 13 3730–3746 Occurrence Handle12388770 Occurrence Handle1:CAS:528:DC%2BD38XotFyrsLc%3D Occurrence Handle10.1091/mbc.E02-04-0213

    Article  PubMed  CAS  Google Scholar 

  • R Palanivelu D Preuss (2000) ArticleTitlePollen tube targeting and axon guidance: parallels in tip growth mechanisms Trends Cell Biol 10 517–524 Occurrence Handle11121743 Occurrence Handle1:CAS:528:DC%2BD3cXot12ltLw%3D Occurrence Handle10.1016/S0962-8924(00)01849-3

    Article  PubMed  CAS  Google Scholar 

  • BA Palevitz A Tiezzi (1992) ArticleTitleOrganization, composition, and function of the generative cell and sperm cytoskeleton Int Rev Cytol 140 149–185 Occurrence Handle10.1016/S0074-7696(08)61096-7

    Article  Google Scholar 

  • S Patel SA Morris CE Adkins G O’Beirne CW Taylor (1997) ArticleTitleCa2+-independent inhibition of inositol trisphosphate receptors by calmodulin: redistribution of calmodulin as a possible means of regulating Ca2+ mobilization Proc Natl Acad Sci USA 94 11627–11632 Occurrence Handle9326661 Occurrence Handle1:CAS:528:DyaK2sXmslejsb0%3D Occurrence Handle10.1073/pnas.94.21.11627

    Article  PubMed  CAS  Google Scholar 

  • ES Pierson J Derksen JA Traas (1986) ArticleTitleOrganization of microfilaments and microtubules in pollen tubes grown in vitro or in vivo in various angiosperms Eur J Cell Biol 41 14–18

    Google Scholar 

  • ES Pierson DD Miller DA Callaham J van Aken G Hackett PK Hepler (1996) ArticleTitleTip-localized calcium entry fluctuates during pollen tube growth Dev Biol 174 160–173 Occurrence Handle8626016 Occurrence Handle1:CAS:528:DyaK28XhsVelurY%3D Occurrence Handle10.1006/dbio.1996.0060

    Article  PubMed  CAS  Google Scholar 

  • AM Prado M Porterfield JA Feijó (2004) ArticleTitleNitric oxide is involved in growth regulation and re-orientation of pollen tubes Development 131 2707–2714 Occurrence Handle15128654 Occurrence Handle1:CAS:528:DC%2BD2cXltlajt78%3D Occurrence Handle10.1242/dev.01153

    Article  PubMed  CAS  Google Scholar 

  • M Potocký M Elias B Profotova Z Novotna O Valentova V Zarsky (2003) ArticleTitlePhosphatidic acid produced by phospholipase D is required for tobacco pollen tube growth Planta 217 122–130 Occurrence Handle12721856

    PubMed  Google Scholar 

  • DJ Powner MJO Wakelam (2002) ArticleTitleThe regulation of phospholipase D by inositol phospholipids and small GTPases FEBS Lett 531 62–64 Occurrence Handle12401204 Occurrence Handle1:CAS:528:DC%2BD38XotVOjtLY%3D Occurrence Handle10.1016/S0014-5793(02)03410-5

    Article  PubMed  CAS  Google Scholar 

  • C Putnam-Evans AC Harmon BA Palevitz M Fechheimer MJ Cormier (1989) ArticleTitleCalcium-dependent protein kinase is localized with F-actin in plant cells Cell Motil Cytoskeleton 12 12–22 Occurrence Handle1:CAS:528:DyaL1MXhsFWgsb0%3D Occurrence Handle10.1002/cm.970120103

    Article  CAS  Google Scholar 

  • C Rato D Monteiro PK Hepler R Malhó (2004) ArticleTitleCalmodulin activity and cAMP signalling modulate growth and apical secretion in pollen tubes Plant J 38 887–897 Occurrence Handle15165182 Occurrence Handle1:CAS:528:DC%2BD2cXlslCntr0%3D Occurrence Handle10.1111/j.1365-313X.2004.02091.x

    Article  PubMed  CAS  Google Scholar 

  • M Raudaskoski H Åström E Laitiainen (2001) ArticleTitlePollen tube cytoskeleton: structure and function J Plant Growth Regul 20 113–130 Occurrence Handle1:CAS:528:DC%2BD3MXlvF2hsrg%3D Occurrence Handle10.1007/s003440010015

    Article  CAS  Google Scholar 

  • ASN Reddy IS Day (2001) ArticleTitleAnalysis of the myosins encoded in the recently completed Arabidopsis thaliana genome sequence Genome Biol 2 IssueID7 research0024.1–0024.17 Occurrence Handle10.1186/gb-2001-2-7-research0024

    Article  Google Scholar 

  • S Romagnoli G Cai M Cresti (2003) ArticleTitleIn vitro assays demonstrate that pollen tube organelles use kinesin-related motor proteins to move along microtubules Plant Cell 15 251–269 Occurrence Handle12509535 Occurrence Handle1:CAS:528:DC%2BD3sXmtFemtQ%3D%3D Occurrence Handle10.1105/tpc.005645

    Article  PubMed  CAS  Google Scholar 

  • SJ Roy TL Holdaway-Clarke GR Hackett JG Kunkel EM Lord PK Hepler (1999) ArticleTitleUncoupling secretion and tip growth in lily pollen tubes: evidence for the role of calcium in exocytosis Plant J 19 379–386 Occurrence Handle10504560 Occurrence Handle1:CAS:528:DyaK1MXms1SrtbY%3D Occurrence Handle10.1046/j.1365-313X.1999.00515.x

    Article  PubMed  CAS  Google Scholar 

  • JJ Rudd VE Franklin-Tong (2001) ArticleTitleUnravelling response-specificity in Ca2+ signalling pathways in plant cells New Phytol 151 7–33 Occurrence Handle1:CAS:528:DC%2BD3MXltFGkurY%3D Occurrence Handle10.1046/j.1469-8137.2001.00173.x

    Article  CAS  Google Scholar 

  • J Šamaj F Baluška D Menzel (2004) ArticleTitleNew signalling molecules regulating root hair tip growth Trends Plant Sci 9 217–220 Occurrence Handle15130546 Occurrence Handle10.1016/j.tplants.2004.03.008 Occurrence Handle1:CAS:528:DC%2BD2cXjvVemtLY%3D

    Article  PubMed  CAS  Google Scholar 

  • RC Schuurink PV Chan RL Jones (1996) ArticleTitleModulation of calmodulin mRNA and protein levels in barley aleurone Plant Physiol 111 371–380 Occurrence Handle12226294 Occurrence Handle1:CAS:528:DyaK28Xjs1Chtrs%3D

    PubMed  CAS  Google Scholar 

  • AP Smertenko C-J Jiang NJ Simmons AG Weeds DR Davies PJ Hussey (1998) ArticleTitleSer6 in the maize actin-depolymerizing factor, ZmADF3, is phosphorylated by a calcium-stimulated protein kinase and is essential for the control of functional activity Plant J 14 187–193 Occurrence Handle9669865 Occurrence Handle1:CAS:528:DyaK1cXjsF2nsrs%3D Occurrence Handle10.1046/j.1365-313X.1998.00107.x

    Article  PubMed  CAS  Google Scholar 

  • RM Smith B Baibakov Y Ikebuchi BH White NA Lambert LK Kaczmarek SS Vogel (2000) ArticleTitleExocytotic insertion of calcium channels constrains compensatory endocytosis to sites of exocytosis J Cell Biol 148 755–767 Occurrence Handle10684256 Occurrence Handle1:CAS:528:DC%2BD3cXhsVOqsL4%3D Occurrence Handle10.1083/jcb.148.4.755

    Article  PubMed  CAS  Google Scholar 

  • CJ Staiger (2000) ArticleTitleSignaling to the actin cytoskeleton in plants Annu Rev Plant Physiol Plant Mol Biol 51 257–288 Occurrence Handle15012193 Occurrence Handle1:CAS:528:DC%2BD3cXlsVymtrY%3D Occurrence Handle10.1146/annurev.arplant.51.1.257

    Article  PubMed  CAS  Google Scholar 

  • CJ Staiger KC Goodbody PJ Hussey R Valenta BK Drøbak CW Lloyd (1993) ArticleTitleThe profilin multigene family of maize: differential expression of three isoforms Plant J 4 631–641 Occurrence Handle8252067 Occurrence Handle1:CAS:528:DyaK2cXjtFKqsb8%3D Occurrence Handle10.1046/j.1365-313X.1993.04040631.x

    Article  PubMed  CAS  Google Scholar 

  • CJ Staiger M Yuan R Valenta PJ Shaw RM Warn CW Lloyd (1994) ArticleTitleMicroinjected profilin affects cytoplasmic streaming in plant cells by rapidly depolymerizing actin microfilaments Curr Biol 4 215–219 Occurrence Handle7922326 Occurrence Handle1:CAS:528:DyaK2cXivFCht7o%3D Occurrence Handle10.1016/S0960-9822(00)00050-6

    Article  PubMed  CAS  Google Scholar 

  • JM Stevenson IY Perera I Heilmann S Persson WF Boss (2000) ArticleTitleInositol signaling and plant growth Trends Plant Sci 5 252–258 Occurrence Handle10838616 Occurrence Handle1:STN:280:DC%2BD3czpsVahtQ%3D%3D Occurrence Handle10.1016/S1360-1385(00)01652-6

    Article  PubMed  CAS  Google Scholar 

  • MW Steer JL Steer (1989) ArticleTitlePollen tube tip growth New Phytol 111 323–358 Occurrence Handle10.1111/j.1469-8137.1989.tb00697.x

    Article  Google Scholar 

  • DA Sweeney A Siddhanta D Shields (2002) ArticleTitleFragmentation and re-assembly of the Golgi apparatus in vitro – a requirement for phosphatidic acid and phosphatidylinositol 4,5-bisphosphate synthesis J Biol Chem 77 3030–3039 Occurrence Handle10.1074/jbc.M104639200 Occurrence Handle1:CAS:528:DC%2BD38XhtVSgtbs%3D

    Article  CAS  Google Scholar 

  • LP Taylor PK Hepler (1997) ArticleTitlePollen germination and tube growth Annu Rev Plant Physiol Plant Mol Biol 48 461–491 Occurrence Handle15012271 Occurrence Handle1:CAS:528:DyaK2sXjs1emsbs%3D Occurrence Handle10.1146/annurev.arplant.48.1.461

    Article  PubMed  CAS  Google Scholar 

  • A Tiezzi A Moscatelli G Cai Bartalesi M Cresti (1992) ArticleTitleAn immunoreactive homolog of mammalian kinesin in Nicotiana tabacum pollen tubes Cell Motil Cytoskeleton 21 132–137 Occurrence Handle1559264 Occurrence Handle1:STN:280:By2B3Mjgs10%3D Occurrence Handle10.1002/cm.970210206

    Article  PubMed  CAS  Google Scholar 

  • AJ Trewavas R Malhó (1997) ArticleTitleSignal perception and transduction: the origin of the phenotype Plant Cell 9 1181–1195 Occurrence Handle12237382 Occurrence Handle1:CAS:528:DyaK2sXlt1Shtbg%3D Occurrence Handle10.1105/tpc.9.7.1181

    Article  PubMed  CAS  Google Scholar 

  • M Vantard L Blanchoin (2002) ArticleTitleActin polymerization processes in plant cells Curr Opin Plant Biol 5 502–506 Occurrence Handle12393012 Occurrence Handle1:CAS:528:DC%2BD38XotVCnt74%3D Occurrence Handle10.1016/S1369-5266(02)00300-X

    Article  PubMed  CAS  Google Scholar 

  • L Vidali PK Hepler (2001) ArticleTitleActin and the pollen tube growth Protoplasma 215 64–76 Occurrence Handle11732066 Occurrence Handle1:CAS:528:DC%2BD3MXltVKrtbo%3D Occurrence Handle10.1007/BF01280304

    Article  PubMed  CAS  Google Scholar 

  • L Vidali E Yokota AY Cheung T Shimmen PK Hepler (1999) ArticleTitleThe 135 kDa actin-bundling protein from Lilium longiflorum pollen is the plant homologue of villin Protoplasma 209 283–291 Occurrence Handle1:CAS:528:DC%2BD3cXhtV2rsLc%3D Occurrence Handle10.1007/BF01453456

    Article  CAS  Google Scholar 

  • L Vidali ST McKenna PK Hepler (2001) ArticleTitleActin polymerization is essential for pollen tube growth Mol Biol Cell 12 2534–2545 Occurrence Handle11514633 Occurrence Handle1:CAS:528:DC%2BD3MXmtFKgu74%3D

    PubMed  CAS  Google Scholar 

  • ID Volotovski SG Sokolovsky OV Molchan MR Knight (1998) ArticleTitleSecond messengers mediate increases in cytosolic calcium in tobacco protoplasts Plant Physiol 117 1023–1030 Occurrence Handle9662545 Occurrence Handle1:CAS:528:DyaK1cXkvVyrs7s%3D Occurrence Handle10.1104/pp.117.3.1023

    Article  PubMed  CAS  Google Scholar 

  • W Wang D Takezawa SB Narasimhulu ASN Reddy BW Poovaiah (1996) ArticleTitleA novel kinesin-like protein with a calmodulin-binding domain Plant Mol Biol 31 87–100 Occurrence Handle8704162 Occurrence Handle1:CAS:528:DyaK28Xks1Khs78%3D Occurrence Handle10.1007/BF00020609

    Article  PubMed  CAS  Google Scholar 

  • GO Wasteneys ME Galway (2003) ArticleTitleRemodeling the cytoskeleton for growth and form: an overview with some new views Annu Rev Plant Biol 54 691–722 Occurrence Handle14503008 Occurrence Handle1:CAS:528:DC%2BD3sXntFSns7w%3D Occurrence Handle10.1146/annurev.arplant.54.031902.134818

    Article  PubMed  CAS  Google Scholar 

  • G Wu Y Gu S Li Z Yang (2001) ArticleTitleA genome-wide analysis of Arabidopsis Rop-interactive CRIB motif-containing proteins that act as Rop GTPase targets Plant Cell 13 2841–2856 Occurrence Handle11752391 Occurrence Handle1:CAS:528:DC%2BD38XktlOgtA%3D%3D Occurrence Handle10.1105/tpc.13.12.2841

    Article  PubMed  CAS  Google Scholar 

  • Z Yang (2002) ArticleTitleSmall GTPases: versatile signaling switches in plants Plant Cell 14 S375–S388 Occurrence Handle12045289 Occurrence Handle1:CAS:528:DC%2BD38XksV2gsr4%3D

    PubMed  CAS  Google Scholar 

  • Z Yang JC Watson (1993) ArticleTitleMolecular cloning and characterization of rho, a ras-related small GTP-binding protein from the garden pea Proc Natl Acad Sci USA 90 8732–8736 Occurrence Handle8378356 Occurrence Handle1:CAS:528:DyaK2cXkslSgtg%3D%3D Occurrence Handle10.1073/pnas.90.18.8732

    Article  PubMed  CAS  Google Scholar 

  • E Yokota (2000) ArticleTitleIdentification and characterization of higher plant myosins responsible for cytoplasmic streaming J Plant Res 113 511–519 Occurrence Handle10.1007/PL00013962

    Article  Google Scholar 

  • E Yokota T Shimmen (1999) ArticleTitleThe 135-kDa actin-bundling protein from lily pollen tubes arranges F-actin into bundles with uniform polarity Planta 209 264–266 Occurrence Handle10436230 Occurrence Handle1:CAS:528:DyaK1MXlt1Gjt74%3D Occurrence Handle10.1007/s004250050631

    Article  PubMed  CAS  Google Scholar 

  • E Yokota S Muto T Shimmen (1999) ArticleTitleInhibitory regulation of higher-plant myosin by Ca2+ ions Plant Physiol 119 231–239 Occurrence Handle9880365 Occurrence Handle1:CAS:528:DyaK1MXmt1GktQ%3D%3D Occurrence Handle10.1104/pp.119.1.231

    Article  PubMed  CAS  Google Scholar 

  • E Yokota K Takahara T Shimmen (1998) ArticleTitleActin-bundling protein isolated from pollen tubes of lily: biochemical and immunocytochemical characterization Plant Physiol 116 1421–1429 Occurrence Handle9536060 Occurrence Handle1:CAS:528:DyaK1cXisFyqtro%3D Occurrence Handle10.1104/pp.116.4.1421

    Article  PubMed  CAS  Google Scholar 

  • E Yokota S Muto T Shimmen (2000) ArticleTitleCalcium-calmodulin suppresses the filamentous actin-binding activity of a 135-kilodalton actinbundling protein isolated from lily pollen tubes Plant Physiol 123 645–654 Occurrence Handle10859194 Occurrence Handle1:CAS:528:DC%2BD3cXktlWntbs%3D Occurrence Handle10.1104/pp.123.2.645

    Article  PubMed  CAS  Google Scholar 

  • SA Young X Wang JE Leach (1996) ArticleTitleChanges in the plasma membrane distribution of rice phospholipase D during resistant interactions with Xanthomonas oryzae pv oryzae Plant Cell 8 1079–1090 Occurrence Handle12239412 Occurrence Handle1:CAS:528:DyaK28XjvFSqsLc%3D Occurrence Handle10.1105/tpc.8.6.1079

    Article  PubMed  CAS  Google Scholar 

  • L Zheng R Krishnamoorthi M Zolkiewski X Wang (2000) ArticleTitleDistinct Ca2+ binding properties of novel C2 domains of plant phospholipase D alpha and beta J Biol Chem 275 19700–19706 Occurrence Handle10777500 Occurrence Handle1:CAS:528:DC%2BD3cXkslahtrY%3D Occurrence Handle10.1074/jbc.M001945200

    Article  PubMed  CAS  Google Scholar 

  • Z-L Zheng Z Yang (2000) ArticleTitleThe Rop GTPase switch turns on polar growth in pollen Trends Plant Sci 5 298–303 Occurrence Handle10871902 Occurrence Handle1:STN:280:DC%2BD3cvgtlCgtw%3D%3D Occurrence Handle10.1016/S1360-1385(00)01654-X

    Article  PubMed  CAS  Google Scholar 

  • RE Zielinski (1998) ArticleTitleCalmodulin and calmodulin-binding proteins in plants Annu Rev Plant Physiol Plant Mol Biol 49 697–725 Occurrence Handle15012251 Occurrence Handle1:CAS:528:DyaK1cXjvVSgs7Y%3D Occurrence Handle10.1146/annurev.arplant.49.1.697

    Article  PubMed  CAS  Google Scholar 

  • L Zonia S Cordeiro J Tupý JA Feijó (2002) ArticleTitleOscillatory chloride efflux at the pollen tube apex has a role in growth and cell volume regulation and is targeted by inositol 3,4,5,6-tetrakisphosphate Plant Cell 14 2233–2249 Occurrence Handle12215517 Occurrence Handle1:CAS:528:DC%2BD38XnsFemurs%3D Occurrence Handle10.1105/tpc.003830

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Departamento de Biologia Vegetal, Instituto de Ciência Aplicada e Tecnologia, Faculdade de Ciências, Universidade de Lisboa, Lisbon

    R. Malhó, Q. Liu, D. Monteiro, C. Rato & L. Camacho

  2. School of Biological and Biomedical Sciences, University of Durham, Durham

    L. Camacho

  3. Departamento de Botânica, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, Coimbra

    A. Dinis

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  1. R. Malhó
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  2. Q. Liu
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  3. D. Monteiro
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  4. C. Rato
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  5. L. Camacho
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  6. A. Dinis
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Correspondence to R. Malhó.

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Correspondence and reprints: Departamento de Biologia Vegetal, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal.

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Malhó, R., Liu, Q., Monteiro, D. et al. Signalling pathways in pollen germination and tube growth. Protoplasma 228, 21–30 (2006). https://doi.org/10.1007/s00709-006-0162-6

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