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Protective Effect of Myricetin Against Experimentally Induced Torsion in Rats

  • ANIMAL AND HUMAN PHYSIOLOGY
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Abstract

Testicular torsion causes ischemia and free radical formation by obstructing vascular flow caused by complete or partial rotation of the testis. Myricetin is a plant-derived flavonoid with various biological effects, such as potent anti-oxidation and anti-inflammatory properties. The study aims to evaluate the protective effects of myricetin on the apoptosis of testicular germ cells after experimental testicular torsion. Twenty-four Wistar albino rats were divided into four groups; Control, Sham, Torsion group (720 deg torsion for 2 h), and Myricetin group (torsion model was created, and myricetin injection (1.5 mg/kg) was done during seven days). End of the experiment, testis tissues were removed and fixed in Bouin’s solution. Crossman’s triple stain was done to determine the histological changes. Also, 8-OHdG and Caspase-3 immunoreactivities were assessed, and TUNEL staining was done. According to the results, we found that 8-OHdG, Caspase-3 immunoreactivity, and TUNEL-positive cells increased, accompanied by several histopathological changes in the torsion group. In contrast, myricetin significantly attenuated testicular injury by reducing apoptosis and DNA damage. These findings suggest that myricetin protects testes against torsion-induced damage in rats, possibly by reducing oxidative stress and inhibiting apoptosis. Our study provides a theoretical basis for further research into maintaining male reproductive health.

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ACKNOWLEDGMENTS

The authors thank Burdur Mehmet Akif Ersoy University Scientific Research Project Office for supporting this study.

Funding

This work was supported by Burdur Mehmet Akif Ersoy University, Scientific Research Project Office under Grant no. 0653-MP-20.

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Authors and Affiliations

  1. Department of Histology and Embryology, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, Turkey

    M. Tatar

  2. Institute of Health Sciences, Burdur Mehmet Akif Ersoy University, Burdur, Turkey

    Z. Polat

  3. Department of Basic Sciences of Histology and Embryology, Faculty of Dentistry, Burdur Mehmet Akif Ersoy University, Burdur, Turkey

    J. Öner

  4. Department of Histology and Embryology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey

    H. Öner

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Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The Burdur Mehmet Akif Ersoy University Animal Experimentation Local Ethics Committee approved this research (no. 2020/690).

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Tatar, M., Polat, Z., Öner, J. et al. Protective Effect of Myricetin Against Experimentally Induced Torsion in Rats. Biol Bull Russ Acad Sci 50, 1338–1350 (2023). https://doi.org/10.1134/S1062359023603221

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