Abstract
The effect of immobilization of nitrile-utilizing bacteria Rhodococcus ruber gt1 and Pseudomonas fluorescens C2 by adhesion on carbon supports on the content of the intracellular ATP immediately after adsorption and after 2 h and 24–48 h after transfer of the adhered cells into a fresh nutrient medium was studied. Adhesion was shown to lead to a decreased concentration of ATP in a cell by one order of magnitude or more in the first hours in a fresh nutrient medium that can be attributed to energetic consumption upon the initiation of biofilm formation. A gradual rise in the quantity of ATP, which was calculated per 1 mg of adsorbed cells, was reported to take place in daily and two-daily biofilms, which confirms the cells remain viable.
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Original Russian Text © Yu.G. Maksimova, V.A. Demakov, 2014, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2014, No. 5, pp. 456–462.
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Maksimova, Y.G., Demakov, V.A. Change in the concentration of intracellular ATP during adhesion of Rhodococcus ruber gt1 and Pseudomonas fluorescens C2 cells on carbon supports. Biol Bull Russ Acad Sci 41, 412–417 (2014). https://doi.org/10.1134/S1062359014050070
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DOI: https://doi.org/10.1134/S1062359014050070