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Raman spectral properties of squamous cell carcinoma of oral tissues and cells

  • Laser Methods in Chemistry, Biology, and Medicine
  • Published:
Laser Physics

Abstract

Early diagnosis is the key of the improved survival rates of oral cancer. Raman spectroscopy is sensitive to the early changes of molecular composition and structure that occur in benign lesion during carcinogenesis. In this study, in situ Raman analysis provided distinct spectra that can be used to discriminate between normal and malignant tissues, as well as normal and cancer cells. The biochemical variations between different groups were analyzed by the characteristic bands by comparing the normalized mean spectra. Spectral profiles of normal, malignant conditions show pronounced differences between one another, and multiple Raman markers associated with DNA and protein vibrational modes have been identified that exhibit excellent discrimination power for cancer sample identification. Statistical analyses of the Raman data and classification using principal component analysis (PCA) are shown to be effective for the Raman spectral diagnosis of oral mucosal diseases. The results indicate that the biomolecular differences between normal and malignant conditions are more obviously at the cellular level. This technique could provide a research foundation for the Raman spectral diagnosis of oral mucosal diseases.

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References

  1. P. N. Notani, Curr. Sci. 81, 465 (2001).

    Google Scholar 

  2. D. M. Parkin, P. Pisani, and J. Ferlay, Int. J. Cancer 80, 827 (1999).

    Article  Google Scholar 

  3. J. B. Epstein, L. Zhang, and M. J. Rosin, J. Can. Dent. Assoc. 68, 610 (2002).

    Google Scholar 

  4. L. E. Meyer and J. Lademann, Laser Phys. Lett. 4, 754 (2007).

    Article  ADS  Google Scholar 

  5. A. Antunes, S. E. Abud, M. L. A. Temperini, and S. L. Morelhao, Laser Phys. Lett. 2, 415 (2005).

    Article  ADS  Google Scholar 

  6. I. M. Vlasova, D. E. Buravtcov, E. V. Dolmatova, V. B. Koshelev, and A. M. Saletsky, Laser Phys. Lett. 3, 401 (2006).

    Article  ADS  Google Scholar 

  7. G. I. Petrov, S. Saltiel, R. D. Heathcote, and V. V. Yakovlev, Laser Phys. Lett. 1, 12 (2004).

    Article  ADS  Google Scholar 

  8. A. G. Shen, Z. X. Liao, L. Goan, Y. Wu, X. H. Wang, Z. Y. Yu, and J. M. Hu, J. Biomed. Opt. 12, 034003 (2007).

    Article  ADS  Google Scholar 

  9. T. C. Bakker Schut, M. J. H. Witjes, H. J. C. M. Sterenborg, O. C. Speelman, J. L. N. Roodenburg, E. T. Marple, H. A. Bruining, and G. J. Puppels, Anal. Chem. 72, 6010 (2000).

    Article  Google Scholar 

  10. G. J. Puppels, F. F. M. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, Nature 347, 301 (1990).

    Google Scholar 

  11. P. V. Zinin, A. Misra, L. Kamemoto, Q. G. Yu, N. J. Hu, and S. K. Sharma, J. Raman Spectrosc. 41, 268 (2010).

    Google Scholar 

  12. I. M. Vlasova and A. M. Saletsky, Laser Phys. 21, 239 (2011).

    Article  ADS  Google Scholar 

  13. I. M. Vlasova and A. M. Saletsky, Laser Phys. 20, 1844 (2010).

    Article  ADS  Google Scholar 

  14. H. Ullah, M. Atif, S. Firdous, M. S. Mehmood, M. Ikram, C. Kurachi, C. Grecco, G. Nicolodelli, and V. S. Bagnato, Laser Phys. Lett. 7, 889 (2010).

    Article  Google Scholar 

  15. M. E. Darvin, A. Patzelt, M. Meinke, W. Sterry, and J. Lademann, Laser Phys. Lett. 6, 229 (2009).

    Article  ADS  Google Scholar 

  16. J. Lademann, P. J. Caspers, A. van der Pol, H. Richter, A. Patzelt, L. Zastrow, M. Darvin, W. Sterry, and J. W. Fluhr, Laser Phys. Lett. 6, 76 (2009).

    Article  ADS  Google Scholar 

  17. M. E. Darvin, I. Gersonde, H. Albrecht, L. Zastrow, W. Sterry, and J. Lademann, Laser Phys. Lett. 4, 318 (2007).

    Article  ADS  Google Scholar 

  18. M. E. Darvin, I. Gersonde, H. Albrecht, M. Meinke, W. Sterry, and J. Lademann, Laser Phys. Lett. 3, 460 (2006).

    Article  ADS  Google Scholar 

  19. P. Chen, Q. Tian, S. J. Baek, X. L. Shang, A. Park, Z. C. Liu, X. Q. Yao, J. Z. Wang, X. H. Wang, Y. Cheng, J. Peng, A. G. Shen, and J. M. Hu, Laser Phys. Lett. 8, 547 (2011).

    Article  ADS  Google Scholar 

  20. H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, Laser Phys. Lett. 8, 78 (2011).

    Article  ADS  Google Scholar 

  21. O. Samek, J. F. M. Al-Marashi, and H. H. Telle, Laser Phys. Lett. 7, 378 (2010).

    Article  ADS  Google Scholar 

  22. W. Xie, L. Su, A. G. Shen, A. Materny, and J. M. Hu, J. Raman Spectrosc. 42, 1248 (2011).

    Article  Google Scholar 

  23. P. Chen, A. G. Shen, X. D. Zhou, and J. M. Hu, Anal. Methods 3, 1257 (2011).

    Article  Google Scholar 

  24. D. E. Gen, K. B. Chernyshov, K. A. Prokhorov, G. Yu. Nikolaeva, E. A. Sagitova, P. P. Pashinin, A. A. Kovalchuk, A. N. Klyamkina, P. M. Nedorezova, V. A. Optov, and B. F. Shklyaruk, Laser Phys. 20, 1354 (2010).

    Article  ADS  Google Scholar 

  25. I. M. Vlasova and A. M. Saletsky, Laser Phys. 19, 2219 (2009).

    Article  ADS  Google Scholar 

  26. G. Bergner, D. Akimov, S. Schlucker, H. Bartelt, B. Dietzek, and J. Popp, Laser Phys. Lett. 8, 541 (2011).

    Article  ADS  Google Scholar 

  27. K. König, H. G. Breunig, R. Bückle, M. Kellner-Höfer, M. Weinigel, E. Büttner, W. Sterry, and J. Lademann, Laser Phys. Lett. 8, 465 (2011).

    Article  Google Scholar 

  28. G. Bergner, E. Vater, D. Akimov, S. Schlucker, H. Bartelt, B. Dietzek, and J. Popp, Laser Phys. Lett. 7, 510 (2010).

    Article  ADS  Google Scholar 

  29. A. P. Oliveira, R. A. Bitar, L. Silveira, R. A. Zangaro, and A. A. Martin, Photomed. Laser Surg. 24, 348 (2006).

    Article  Google Scholar 

  30. R. Malini, K. Venkatakrishna, J. Kurien, L. Rao, V. B. Kartha, and C. M. Krishna, Biopolymers 81, 179 (2006).

    Article  Google Scholar 

  31. D. C. G. de Veld, T. C. B. Schut, M. Skurichina, M. J. H. Witjes, J. E. Van der Wal, J. L. N. Roodenburg, and H. J. C. M. Sterenborg, Lasers Med. Sci. 19, 203 (2005).

    Article  Google Scholar 

  32. C. M. Krishna, G. D. Sockalingum, J. Kurien, L. Rao, L. Venteo, M. Pluot, M. Manfait, and V. B. Kartha, Appl. Spectrosc. 58, 1128 (2004).

    Article  ADS  Google Scholar 

  33. Y. Li, Z. N. Wen, L. J. Li, M. L. Li, N. Gao, and Y. Z. Guo, J. Raman Spectrosc. 41, 142 (2010).

    Google Scholar 

  34. A. Mahadevan-Jansen and R. Richards-Kortum, J. Biomed. Opt. 1, 31 (1996).

    Google Scholar 

  35. J. D. Gelder, K. D. Gussem, P. Vandenabeele, and L. Moens, J. Raman Spectrosc. 38, 1133 (2007).

    Article  ADS  Google Scholar 

  36. A. M. Proenza, J. Oliver, A. Palou, and P. Roca, J. Nutr. Biochem. 14, 133 (2003).

    Article  Google Scholar 

  37. S. L. Hu, G. Shen, S. Yin, W. P. Xu, and B. Hu, Adv. Ther. 26, 886 (2009).

    Article  Google Scholar 

  38. A. Tankiewicz, D. Dziemianczyk, P. Buczko, I. J. Szarmach, S. Z. Grabowska, and D. Pawlak, Adv. Med. Sci. 51(Suppl. 1), 221 (2006).

    Google Scholar 

  39. C. J. Frank, R. L. McCreery, and D. C. Redd, Anal. Chem. 67, 777 (1995).

    Article  Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China

    L. Su, Y. Chen, P. Chen, A. G. Shen, X. H. Wang, X. D. Zhou & J. M. Hu

  2. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430072, China

    Y. F. Sun, J. Jia & Y. F. Zhao

Authors
  1. L. Su
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  2. Y. F. Sun
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  3. Y. Chen
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  4. P. Chen
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  5. A. G. Shen
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  6. X. H. Wang
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  7. J. Jia
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  8. Y. F. Zhao
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  9. X. D. Zhou
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  10. J. M. Hu
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Corresponding author

Correspondence to A. G. Shen.

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Original Text © Astro, Ltd., 2012.

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Su, L., Sun, Y.F., Chen, Y. et al. Raman spectral properties of squamous cell carcinoma of oral tissues and cells. Laser Phys. 22, 311–316 (2012). https://doi.org/10.1134/S1054660X12010185

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  • DOI: https://doi.org/10.1134/S1054660X12010185

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