Abstract
Autofluorescence spectroscopy and Raman spectroscopy have been suggested for lesion diagnostics. We investigate the information contained in autofluorescence and Raman spectra recorded from oral tissue slices of various lesion types. Thirty-seven human oral mucosa lesions were biopsied and freeze-dried. Complete autofluorescence images and spectra were recorded from 20 μm sections. Raman spectra were acquired from the same positions for 12 of the sections. Cluster analysis was applied to find any relationship between spectral shape and lesion type or cell layer. Autofluorescence images showed high intensities for keratin layers and connective tissue, but hardly any for the epithelium. Autofluorescence spectra were centered around 520 nm and did not show specific spectral features. No clustering with regard to lesion type or cell layer was observed. Raman spectra allowed for reliable classification into cell layers, but differences between lesion types were not significant in this study. Autofluorescence spectra of freeze-dried oral mucosa sections did not contain useful information. A more comprehensive study is required for Raman spectra.
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Acknowledgements
This work was supported by the Dutch Cancer Society (“Nederlandse Kanker Bestrijding”), grant AZG-99-1869, and the Dutch Technology Foundation (“STW”), grant RRN 5316. We are grateful to Bastiaan Kruijt for his valuable work on the design and construction of the microscopic setup, and to Marco Abma and colleagues from the Pathology department for storing and preparing the tissue sections. Contract grant sponsor: Dutch Cancer Society (Nederlandse Kanker Bestrijding); Contract grant number: RUG-99-1869.
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de Veld, D.C.G., Bakker Schut, T.C., Skurichina, M. et al. Autofluorescence and Raman microspectroscopy of tissue sections of oral lesions. Lasers Med Sci 19, 203–209 (2005). https://doi.org/10.1007/s10103-004-0325-7
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DOI: https://doi.org/10.1007/s10103-004-0325-7