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Development of Molecular Markers Based on the L1 Retrotransposon Insertion Polymorphisms in Pigs (Sus scrofa) and Their Association with Economic Traits

  • MOLECULAR GENETICS
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Abstract

Molecular markers based on retrotransposon insertion polymorphisms (RTIPs) have great potential in studies of animal genetics and breeding. In this study, L1 RTIP markers were developed and evaluated by using microsatellite primers combined with L1 specific primers, and were further evaluated in four Chinese and three imported domestic pig breeds. Eight L1RTIP markers were obtained, and uneven distribution was observed among these breeds, with the Sujiang breed exhibiting the highest level of polymorphism with seven polymorphic sites, followed by Jiangquhai, Meishan, and Duroc with six, four, and four polymorphic sites, respectively. Both Landrace and Yorkshire pigs exhibited three polymorphic sites, while Chinese inbred Bama pig showed the lowest level of polymorphism with only one polymorphic site. Three polymorphic makers (L1-31-2, L1-31-5, and L1-31-11) were strongly associated with the economic traits in 462 Yorkshire pigs, with the L1-31-5+ genotype females had a significantly greater alive litter size (11.44 ± 2.80) compared with pigs with the L1-31-5– genotype (9.96 ± 2.89) (P < 0.05). L1-31-11– genotype pigs had significantly younger age at 100 kg of body weight (160.20 ± 10.26 days) than the L1-31-11+counter parts (162.47 ± 9.71 days) (P < 0.05). In summary, we demonstrated that it is feasible to develop markers based on L1 RTIP sin the pig, and these markers may have greater potential in the application of pig genetics and breeding compared to non-selective conventional breeding.

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Funding

This work was supported by the Natural Science Foundation of China (31572364 and 31872977); the Priority Academic Program Development of Jiangsu Higher Education Institutions; the National Development and Reform Commission Special Breeding Projects; and the Postgraduate Research and Practical Innovation Program of Jiangsu Province (XKYCX17_058).

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    Authors and Affiliations

    1. Institute of Animal Mobilome and Genome, College of Animal Science and Technology, Yangzhou University, 225009, Yangzhou, Jiangsu, China

      W. Wang, C. Chen, X. Wang, L. Zhang, D. Shen, S. Wang, B. Gao & C. Song

    2. Life Science Center, University of Missouri, 65211, Columbia, MO, USA

      J. Mao

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    1. W. Wang
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    2. C. Chen
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    3. X. Wang
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    W. Wang and C. Chen have contributed equally to this work.

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    Correspondence to C. Song.

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    Conflict of interest. The authors declare no competing financial interests.

    Statement on the welfare of animals. Animal care and use was approved by the University of Yangzhou Animal Care and Use Committee.

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    Wang, W., Chen, C., Wang, X. et al. Development of Molecular Markers Based on the L1 Retrotransposon Insertion Polymorphisms in Pigs (Sus scrofa) and Their Association with Economic Traits. Russ J Genet 56, 183–191 (2020). https://doi.org/10.1134/S1022795420020131

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