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Characterization of fertility restoration in alloplasmic lines derived from hybridization of self-fertilized offspring of barley-wheat (Hordeum vulgare L. × Triticum aestivum L.) amphiploid with common wheat varieties Saratovskaya 29 and Pyrotrix 28

  • Plant Genetics
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Abstract

The problems of fertility restoration in the progeny of barley-wheat hybrids (H. vulgare × T. aestivum) are explained by incompatibility between the cytoplasm of cultivated barley and the nuclear genome of common wheat. Appropriate models for studying these problems are alloplasmic lines that combine the cytoplasm of barley and the nuclear genome of wheat. In this work, the differences of fertility restoration in alloplasmic common wheat lines (H. vulgare)-T. aestivum were studied depending on the influence of wheat varieties Saratovskaya 29 (Sar29) and Pyrotrix 28 (Pyr28) used to produce these lines. The alloplasmic lines were created using hybrids between the 48-chromosome offspring (Amph1) of the barley-wheat amphiploid H. vulgare (ya-319) × T. aestivum (Sar29) and these wheat varieties. Backcrossing of the Amph1 (2n = 48) × Sar29 hybrid with the wheat variety Sar29 resulted in the complete sterility in the (H. vulgare)-Sar29 line, which suggests the incompatibility of the nuclear genome of the common wheat variety Sar29 with the cytoplasm of H. vulgare. Crossing of Amph1 (2n = 48) with Pyr28 resulted in the restoration of self-fertility in the hybrid with 2n = 44. In the alloplasmic lines (2n = 42) formed based on plants of the self-fertilized generations of this hybrid, the barley chromosomes were eliminated, and recombination between the nuclear genomes of the parental wheat varieties Sar29 and Pyr28 took place. Alloplasmic recombinant lines (H. vulgare)-T. aestivum with different levels of fertility were isolated. As was shown by the SSR analysis, differences in the fertility between these lines are determined by differences in the content of the genetic material from the wheat varieties Sar29 and Pyr28. The complete restoration of fertility in these alloplasmic recombinant lines is accompanied by the formation of a nuclear genome in which the genetic material of Pyr28 significantly prevails. The conclusion is made that the common wheat variety Pyrotrix 28 is a carrier of a gene (or genes), which determines the restoration of common wheat fertility on the cytoplasm of cultivated barley.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    L. A. Pershina, E. P. Devyatkina, N. V. Trubacheeva, L. A. Kravtsova & O. B. Dobrovol’skaya

  2. Department of Cytology and Genetics, Novosibirsk State University, Novosibirsk, 630090, Russia

    L. A. Pershina

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  1. L. A. Pershina
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  2. E. P. Devyatkina
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  3. N. V. Trubacheeva
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  4. L. A. Kravtsova
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  5. O. B. Dobrovol’skaya
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Correspondence to L. A. Pershina.

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Original Russian Text © L.A. Pershina, E.P. Devyatkina, N.V. Trubacheeva, L.A. Kravtsova, O.B. Dobrovol’skaya, 2012, published in Genetika, 2012, Vol. 48, No. 12, pp. 1372–1379.

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Pershina, L.A., Devyatkina, E.P., Trubacheeva, N.V. et al. Characterization of fertility restoration in alloplasmic lines derived from hybridization of self-fertilized offspring of barley-wheat (Hordeum vulgare L. × Triticum aestivum L.) amphiploid with common wheat varieties Saratovskaya 29 and Pyrotrix 28. Russ J Genet 48, 1184–1190 (2012). https://doi.org/10.1134/S1022795412120101

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  • DOI: https://doi.org/10.1134/S1022795412120101

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