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Chromosome engineering in wheat to restore male fertility in the msH1 CMS system

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Abstract

Pollen fertility restoration of the CMS phenotype caused by H. chilense cytoplasm in wheat was associated with the addition of chromosome 6HchS from H. chilense accession H1. In order to develop an euploid restored line, different genomic combinations substituting the 6HchS arm for another homoeologous chromosome in wheat were evaluated, with the conclusion that the optimal combination was the translocation T6HchS·6DL. The double translocation T6HchS·6DL in H. chilense cytoplasm was obtained. This line is fertile and stable under different environmental conditions. However, a single dose of the T6HchS·6DL translocation is insufficient for fertility restoration when chromosome 6D is also present. Restoration in the msH1 system is promoted by interaction between two or more genes, and in addition to the restorer of fertility (Rf) located on chromosome 6HchS, one or more inhibitor of fertility (Fi) genes may be present in chromosome 6DL.

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Acknowledgments

We thank Dr. P. Lazzeri (Agrasys S.L.) for revision and correction of the English in this manuscript. This work was supported by MICINN (Ministerio de Ciencia e Innovación) projects AGL2006-07703 and AGL2007-65685-C02-01 of the Spanish Government.

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  1. Departamento de Mejora Genética Vegetal, Instituto de Agricultura Sostenible (C.S.I.C.), Apdo. 4084, 14080, Córdoba, Spain

    Azahara C. Martín, Sergio G. Atienza, María C. Ramírez, Francisco Barro & Antonio Martín

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  1. Azahara C. Martín
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  2. Sergio G. Atienza
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  3. María C. Ramírez
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  4. Francisco Barro
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  5. Antonio Martín
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Correspondence to Azahara C. Martín.

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Martín, A.C., Atienza, S.G., Ramírez, M.C. et al. Chromosome engineering in wheat to restore male fertility in the msH1 CMS system. Mol Breeding 24, 397–408 (2009). https://doi.org/10.1007/s11032-009-9301-z

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  • DOI: https://doi.org/10.1007/s11032-009-9301-z

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