Skip to main content
SpringerLink
Log in

Comparative analysis of genome sizes of Streptococcus thermophilus strains

  • Genetics of Microorganisms
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

Twenty-five Streptococcus thermophilus isolates were analyzed using pulse-field gel electrophoresis (PFGE) and gene restriction profile analysis techniques. 16S rRNA gene sequences of the isolates were almost 100% homologous. However, genomic fingerprinting analysis has shown variability in both genome size and restriction fragments length. The genomes varied from 1417 to 2075 kb resulting in the difference between marginal genome sizes in about 600 kb. The results are indicative of Streptococcus thermophilus intraspecies genetic polymorphism, the origin of which requires further investigation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lan, R. and Reeves, P.R., Intraspecies Variation in Bacterial Genomes: The Need for a Species Genome Concept, Trends Microbiol., 2000, vol. 8, no. 9, pp. 396–401.

    Article  PubMed  CAS  Google Scholar 

  2. Botina, S.G., Trenina, M.A., Tsygankov, Yu.D., and Sukhodolets, V.V., Comparison of Genotypic and Biochemical Characteristics of the Streptococcus thermophilus Strains Isolated from Dairy Products, Prikl. Biokhim. Mikrobiol., 2007, vol. 43, no. 6.

  3. Olive, D.M. and Bean, P., Principles and Applications of Methods for DNA-Based Typing of Microbial Organisms: Minireview, J. Clin. Microbiol., 1999, vol. 37, no. 6, pp. 1661–1669.

    PubMed  CAS  Google Scholar 

  4. Botina, S.G., Tsygankov, Yu.D., and Sukhodolets, V.V., Identification of Industrial Strains of Lactic Acid Bacteria by Methods of Molecular Genetic Typing, Russ. J. Genet., 2006, vol. 42, no. 12, pp. 1367–1379.

    Article  CAS  Google Scholar 

  5. Sukhodolets, V.V., Botina, S.G., Lysenko, A.M., and Trenina, M.A., Lactic Acid Enterococci Enterococcus faesium and Enterococcus durans: Diversity of Nucleotide Sequences in 16S rRNA Genes, Mikrobiologiya, 2005, vol. 74, no. 6, pp. 810–815.

    CAS  Google Scholar 

  6. Botina, S.G., Lysenko, A.M., Sukhodolets, V.V., and Trenina, M.A., Comparison of Genomes and Strains of Lactic Acid Bacteria Streptococcus thermorhilus of Different Origin, Mikrobiologiya, 2002, vol. 71, no. 6, pp. 819–823.

    CAS  Google Scholar 

  7. Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Lab., 1989.

    Google Scholar 

  8. Trenina, M.A., Lysenko, A.M., Akhverdyan, V.Z., and Mchedlishvili, E.B., Study of Intraspecific Variability in Bacteria Lactococcus lactis Relative to Adaptation to High Environmental Acidity, Mikrobiologiya, 2006, vol. 75, no. 1, pp. 118–126.

    CAS  Google Scholar 

  9. Salzano, G., Moschetti, G., Villani, F., and Coppola, S., Biotyping of Streptococcus thermorhilus Strains by DNA Fingerprinting, Res. Microbiol., 1993, vol. 144, no. 5, pp. 381–387.

    Article  PubMed  CAS  Google Scholar 

  10. Boutrou, R., Thuault, D., and Bourgeois, C.M., Identification and Characterization of Streptococcus thermorhilus Strains by Pulsed-Field Gel Electrophoresis, J. Appl. Bacteriol., 1995, vol. 79, pp. 454–458.

    CAS  Google Scholar 

  11. Le Bourgeois, P., Lautier, M., and Ritzenthaler, P., Chromosome Mapping in Lactic Acid Bacteria, FEMS Microbiol. Rev., 1993, vol. 12, pp. 109–124.

    Article  PubMed  Google Scholar 

  12. Bolotin, A., Benoit, Q., Renault, P., et al., Complete Sequence and Comparative Genome Analysis of the Dairy Bacterium Streptococcus thermorhilus, Genome Res., 2004, vol. 11, pp. 731–753.

    Article  Google Scholar 

  13. Le Bourgeois, P., Lautier, M., Mata, M., and Ritzenthaler, P., Physical and Genetic Map of the Chromosome of Lactococcus lactis subsp. lactis IL1403, J. Bacteriol., 1992, vol. 174, no. 21, pp. 6752–6762.

    PubMed  Google Scholar 

  14. Roussel, Y., Bourgoin, F., Guedon, G., et al., Analysis of the Genetic Polymorphism between Three Streptococcus thermorhilus Strains by Comparing Their Physical and Genetic Organization, Microbiology, 1997, vol. 143, pp. 1335–1343.

    Article  PubMed  CAS  Google Scholar 

  15. Yother, J., Trieu-Cuot, P., Klaenhammer, T.R., and de Vos, W.M., Genetics of Streptococci, Lactococci, and Enterococci: Review of the Sixth International Conference, J. Bacteriol., 2002, vol. 184, no. 22, pp. 6085–6092.

    Article  PubMed  CAS  Google Scholar 

  16. Flint, S.H., Ward, J.H., and Brooks, J.D., Streptococcus waius sp. nov., a Thermophilic Streptococcus from a Biofilm, Int. J. Syst. Bacteriol., 1999, vol. 49, pp. 759–767.

    Article  PubMed  CAS  Google Scholar 

  17. Manachini, P.L., Flint, S.H., Ward, L.J.H., et al., Comparison between Streptococcus macedonicus and Streptococcus waius Strains and Reclassification of Streptococcus waius as Streptococcus macedonicus, Int. J. Systematic Evol. Microbiol., 2002, vol. 52, pp. 945–951.

    Article  CAS  Google Scholar 

  18. Tsakalidou, E., Zoidou, E., Pot, B., et al., Identification of Streptococci from Greek Kasseri Cheese and Description of Streptococcus macedonicus sp. nov., Intern. J. Systematic Bacteriol., 1998, vol. 48, pp. 519–527.

    CAS  Google Scholar 

  19. Tanskanen, E.I., Tulloch, D.L., Hiller, A.J., and Davidson, B.E., Pulsed-Field Gel Electrophoresis of SmaI Digests of Lactococcal Genomic DNA, a Novel Method of Strain Identification, Appl. Environ. Microbiol., 1990, vol. 56, no. 10, pp. 3105–3111.

    PubMed  CAS  Google Scholar 

  20. Lan, R. and Reeves, P.R., Gene Transfer Is a Major Factor in Bacterial Evolution, Mol. Biol. Evol., 1996, vol. 13, no. 1, pp. 47–55.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Research Institute of Genetics and Selection of Industrial Microorganisms (GosNIIGenetika), Moscow, 113545, Russia

    S. G. Botina, O. V. Piksasova, Yu. D. Tsygankov & V. V. Sukhodolets

Authors
  1. S. G. Botina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. V. Piksasova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yu. D. Tsygankov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. V. Sukhodolets
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Original Russian Text © S.G. Botina, O.V. Piksasova, Yu.D. Tsygankov, V.V. Sukhodolets, 2007, published in Genetika, 2007, Vol. 43, No. 7, pp. 891–897.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Botina, S.G., Piksasova, O.V., Tsygankov, Y.D. et al. Comparative analysis of genome sizes of Streptococcus thermophilus strains. Russ J Genet 43, 736–741 (2007). https://doi.org/10.1134/S1022795407070046

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795407070046

Keywords

Search

Navigation