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Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl-lipid desaturases and thermostable lichenase

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Abstract

We established transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 Δ12 (desA) acyllipid desaturase and Synechococcus vulcanus Δ9 (desC) acyllipid desaturase with or without sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of a constitutive promoter. Reliable increase of linoleic acid portion (C18:2; Δ9,12) accompanied with decrease of α-linolenic acid (C18:3; Δ9,12,15) relative amount was detected for plants expressing hybrid desA::licBM3 gene. No reliable changes were detected in fatty acid profiles and unsaturation index of plants transformed with Δ9 desaturase gene desC::licBM3 lacking signals of intracellular targeting while expression of this gene with Arabidopsis thaliana Rubisco small subunit transit peptide sequence caused growth of C18:3 α-linolenic acid part simultaneously with reduction of C18:2 linoleic acid part, as well as increase of unsat-uration index. No changes in relative amount of Δ9-monounsaturated fatty acids were observed in any of studied lines. All plants expressing desaturase genes exhibited enhanced levels of superoxide dismutase (SOD) activity after cold treatment in contrast to control lines with suppressed SOD activity after cold treatment.

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Abbreviations

ACP:

acyl carrier protein

CoA:

coenzyme A

FA:

fatty acid

GFP:

green fluorescent protein

RTP:

transit peptide of Rubisco small subunit

SOD:

superoxide dismutase

TSP:

total soluble protein

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Authors and Affiliations

  1. Institute of Cell Biology and Genetic Engineering National Academy of Science of Ukraine, Kyiv, Ukraine

    I. M. Gerasymenko, L. A. Sakhno, T. N. Kyrpa & Y. V. Sheludko

  2. Zabolotny Institute of Microbiology and Virology National Academy of Science of Ukraine, Kyiv, Ukraine

    A. M. Ostapchuk

  3. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    T. A. Hadjiev & I. V. Goldenkova-Pavlova

Authors
  1. I. M. Gerasymenko
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  2. L. A. Sakhno
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  3. T. N. Kyrpa
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  4. A. M. Ostapchuk
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  5. T. A. Hadjiev
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  6. I. V. Goldenkova-Pavlova
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  7. Y. V. Sheludko
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Correspondence to I. V. Goldenkova-Pavlova.

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Gerasymenko, I.M., Sakhno, L.A., Kyrpa, T.N. et al. Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl-lipid desaturases and thermostable lichenase. Russ J Plant Physiol 62, 283–291 (2015). https://doi.org/10.1134/S1021443715030073

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  • DOI: https://doi.org/10.1134/S1021443715030073

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