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Modulation of polyamine levels in ginseng hairy root cultures subjected to salt stress

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Abstract

Modulation of differential gene expression and change of polyamine content by salt stress are analyzed for the first time in a well-known medicinal plant, Panax ginseng C.A. Meyer. Three ginseng genes (PgSPD, PgSAMDC, and PgADC) involved in polyamine biosynthesis showed differential up-regulation patterns after 1 and 7 days of salt treatments. The modulation of gene expression resulted in the elevation of total polyamine content with relatively high levels of spermidine and spermine, while putrescine level diminished depending on the salt concentration. Conversely, salt stress led to a significant increase in diamine oxidase and subsequent decline in polyamine oxidase. The proline content caused by salinity follows a similar pattern as the total polyamine content and exogenous spermidine also resulted in the alleviation of proline content under salinity. Further, polyamine biosynthesis inhibitors, such as cyclohexylamine and methylglyoxal bis-(guanylhydrazone) mediated down-regulation of PgSPD and PgSAMDC, and affected cellular polyamine levels. Thus, polyamines may enhance the ginseng plant tolerance in response to the salt stress by increasing the levels of endogenous polyamines.

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Abbreviations

CHA:

cyclohexylamine

DAO:

diamine oxidase

EST:

expressed sequence tag

MGBG:

methylglyoxal bis-(guanylhydrazone)

PA:

polyamine

PAO:

polyamine oxidase

Put:

putrescine

qRT-PCR:

quantitative real-time Reverse Transcription-Polymerase Chain Reaction

Spd:

spermidine

SPD:

spermidine synthase

Spm:

spermine

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Authors and Affiliations

  1. Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, Seocheon, Giheung-gu Yongin-si, Gyeonggi-do, 449-701, South Korea

    S. Parvin, O. R. Lee, G. Sathiyaraj, A. Khorolragchaa, Y. J. Kim & D. C. Yang

  2. Department of Agroforestry and Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh

    S. Parvin & Md. G. Miah

Authors
  1. S. Parvin
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  2. O. R. Lee
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  3. G. Sathiyaraj
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  4. A. Khorolragchaa
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  5. Y. J. Kim
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  6. Md. G. Miah
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  7. D. C. Yang
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Correspondence to D. C. Yang.

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Parvin, S., Lee, O.R., Sathiyaraj, G. et al. Modulation of polyamine levels in ginseng hairy root cultures subjected to salt stress. Russ J Plant Physiol 59, 757–765 (2012). https://doi.org/10.1134/S102144371206012X

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  • DOI: https://doi.org/10.1134/S102144371206012X

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