Abstract
The role of endogenous GA3 and its application to seed development in two cotton genotypes Hybrid-6 (H-6) (big seeds) and Gujarat cotton 13 (G. Cot) (small seeds) was studied. Kernel and seed coat were subjected to growth analysis in terms of dry weight, water amount, and rates of dry matter accumulation and water uptake. H-6 kernel had manifold higher dry weight and water amount than G. Cot. Seed coat of both genotypes had similar dry weight at maturity, but the maximum rates of dry matter accumulation and water uptake were distinctly higher in H-6. According to growth analysis, development of seed kernel and coat was subdivided into four phases, i.e., cell division, cell elongation, dry matter accumulation and maturation. Endogenous GA3 level was estimated in kernel and seed coat by indirect ELISA using antibodies raised against GA3. GA3 amount per seed components was higher in the seed kernel of H-6 than of G. Cot, except 33 and 36 days after anthesis in kernel. H-6 seed coat had the higher amount of GA3 during cell division phase than that of G. Cot. Close correlation between in vivo GA3 level and water amount was recorded in both seed components. With GA3 or GA3 + NAA treatments in ovule culture, higher promotion in dry weight, water amount and seed size was noted in G. Cot than in H-6 suggesting that G. Cot is more deficient in endogenous GA3. The greatest stimulation of parameters studied was obtained in ovule culture with GA3 + NAA. When GA3 or GA3 + NAA was applied, initial significant difference in water amount and seed size was nullified. Data presented in this study indicated that GA3 regulates cell expansion through the water uptake by cotton seed.
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Abbreviations
- DMA:
-
dry matter accumulation
- DMF:
-
dimethylformamide
- DPA:
-
days post anthesis
- G. Cot:
-
Gujarat cotton-13, H-6-Hybrid-6
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Original Russian Text © K.R. Bhatt, V.S. Thaker, 2008, published in Fiziologiya Rastenii, 2008, Vol. 55, No. 6, pp. 895–900.
This text was submitted by the authors in English.
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Bhatt, K.R., Thaker, V.S. Relationship between gibberellic acid and water amount in the cotton seed. Russ J Plant Physiol 55, 808–813 (2008). https://doi.org/10.1134/S1021443708060101
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DOI: https://doi.org/10.1134/S1021443708060101