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Defense response mechanisms of ginseng callus cultures induced by Yersinia pseudotuberculosis, a human pathogen

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Abstract

The effect of Yersinia pseudotuberculosis, the bacterial pathogen affecting humans and animals, on growth of ginseng (Panax ginseng C.A. Mey) cell cultures was studied. The bacteria strongly induced the expression of phenylalanine ammonia-lyase and β-1,3-glucanase, the proteins encoded by the defense-related genes of ginseng and inhibited the normal ginseng callus growth but did not affect the resistant cell cultures. The thermostable and thermolabile protein toxins of these bacteria are lethal to mice when induced parentherally, and they also induced the expression of the defense-related genes in ginseng callus cultures. At the same time, the ginseng cells completely suppressed the bacterial cell growth. These data suggest that the ginseng cells recognized the yersinia and developed the immune response to this pathogen. The interaction between the ginseng cells and Y. pseudotuberculosis is similar to the hypersensitive response of plants to plant pathogens.

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Abbreviations

Avr:

avirulence

CFU:

colony-forming unit

HR:

hypersensitive response

hrp :

hypersensitive response and pathogenicity genes

PAL:

phenylalanine ammonia-lyase

Pgglu1:

Panax ginseng β-1,3-glucanase

PgPAL:

P. ginseng PAL

TLTYp:

thermolabile toxin of Yersinia pseudotuberculosis

TSTYp:

thermostable toxine of Y. pseudotuberculosis

Yop:

Yersinia outer protein

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Authors and Affiliations

  1. Institute of Epidemiology and Microbiology, Siberian Division, Russian Academy of Medical Sciences, ul. Sel’skaya 1, Vladivostok, 690028, Russia

    E. V. Persiyanova & N. F. Timchenko

  2. Institute of Biology and Soil Sciences, Far East Division, Russian Academy of Sciences, Vladivostok, Russia

    K. V. Kiselev, V. P. Bulgakov, G. K. Chernoded & Yu. N. Zhuravlev

Authors
  1. E. V. Persiyanova
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  2. K. V. Kiselev
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  3. V. P. Bulgakov
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  4. N. F. Timchenko
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  5. G. K. Chernoded
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  6. Yu. N. Zhuravlev
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Correspondence to E. V. Persiyanova.

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Original Russian Text © E.V. Persiyanova, K.V. Kiselev, V.P. Bulgakov, N.F. Timchenko, G.K. Chernoded, Yu.N. Zhuravlev, 2008, published in Fiziologiya Rastenii, 2008, Vol. 55, No. 6, pp. 834–841.

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Persiyanova, E.V., Kiselev, K.V., Bulgakov, V.P. et al. Defense response mechanisms of ginseng callus cultures induced by Yersinia pseudotuberculosis, a human pathogen. Russ J Plant Physiol 55, 748–755 (2008). https://doi.org/10.1134/S1021443708060034

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  • DOI: https://doi.org/10.1134/S1021443708060034

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