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Inhibitory effect of the Agrobacterium rhizogenes rolC gene on rabdosiin and rosmarinic acid production in Eritrichium sericeum and Lithospermum erythrorhizon transformed cell cultures

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Abstract

Rabdosiin and related caffeic acid metabolites have been proposed as active pharmacological agents demonstrating potent anti-HIV and antiallergic activities. We transformed Eritrichium sericeum and Lithospermum erythrorhizon seedlings by the rolC gene, which has been recently described as an activator of plant secondary metabolism. Surprisingly, the rolC-transformed cell cultures of both plants yielded two- to threefold less levels of rabdosiin and rosmarinic acid (RA) than respective control cultures. This result establishes an interesting precedent when the secondary metabolites are differently regulated by a single gene. We show that the rolC gene affects production of rabdosiin and RA irrespective of the methyl jasmonate (MeJA)-mediated and the Ca2+-dependent NADPH oxidase pathways. Cantharidin, an inhibitor of serine/threonine phosphatases, partly diminishes the rolC-gene inhibitory effect that indicates involvement of the rolC-gene-mediated signal in plant regulatory controls, mediated by protein phosphatases. We also show that the control MeJA-stimulated E. sericeum root culture produces (−)-rabdosiin up to 3.41% dry weight, representing the highest level of this substance for plant cell cultures reported so far.

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Abbreviations

CAM:

Caffeic acid metabolites

DPI:

Diphenylene iodonium

MeJA:

Methyl jasmonate

RA:

Rosmarinic acid

ROS:

Reactive oxygen species

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Acknowledgements

This work was supported by grants 03-04-49515 and 03-04-48102 of the Russian Foundation for Basic Research, grants 04-1-05-002 and 04-1-05-004 from the Grant Programmes “Molecular and cell biology” and “Fundamental sciences for medicine” of the Russian Academy of Sciences.

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Authors and Affiliations

  1. Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, 159 Stoletija Street, Vladivostok, 690022, Russia

    Victor P. Bulgakov, G. K. Tchernoded, K. V. Kiselev & Yu. N. Zhuravlev

  2. Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, Vladivostok, 690022, Russia

    M. V. Veselova & S. A. Fedoreyev

Authors
  1. Victor P. Bulgakov
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  2. M. V. Veselova
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  3. G. K. Tchernoded
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  4. K. V. Kiselev
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  5. S. A. Fedoreyev
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  6. Yu. N. Zhuravlev
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Correspondence to Victor P. Bulgakov.

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Bulgakov, V.P., Veselova, M.V., Tchernoded, G.K. et al. Inhibitory effect of the Agrobacterium rhizogenes rolC gene on rabdosiin and rosmarinic acid production in Eritrichium sericeum and Lithospermum erythrorhizon transformed cell cultures. Planta 221, 471–478 (2005). https://doi.org/10.1007/s00425-004-1457-5

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  • DOI: https://doi.org/10.1007/s00425-004-1457-5

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