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Molecular Biology

, Volume 52, Issue 3, pp 446–452 | Cite as

Effect of Sodium Selenite on Gene Expression of SELF, SELW, and TGR Selenoproteins in Adenocarcinoma Cells of the Human Prostate

  • E. G. Varlamova
  • M. V. Goltyaev
  • J. P. Kuznetsova
Molecular Cell Biology
  • 35 Downloads

Abstract

Selenium is an essential trace element, the deficiency of which leads to the development of several serious diseases, including male infertility, prostate cancer, etc. It has been shown that oxidative stress contributes to the progression of prostate cancer, and antioxidants such as selenium and vitamin E can significantly reduce the risk of this disease. Sodium selenite, one of the selenium compounds that induce the formation of reactive oxygen species, is considered as a potential anticancer agent. The SS concentrations that lead to a decrease in the viability of human prostate adenocarcinoma cells (line Du-145) have been selected, and the effect of sodium selenite on the expression of mRNA of the SELV, SELW, and TGR selenocysteine proteins in these cells has been analyzed.

Keywords

selenium mammalian selenocysteine-containing proteins prostate cancer sodium selenite 

Abbreviations

GAPDH

glyceraldehyde-3-dehydrogenase

GPX

glutathione peroxidase

IPTGP

isopropyl β-D-1-thiogalactopyranoside

ORF

open reading frame

ROS

reactive oxygen species

Se

selenium

Sec

selenocysteine

SELV and SELW

selenocysteine-containing proteins V and W

SS

sodium selenite

TGR

thioredoxin glutathione reductase

EPR

endoplasmic reticulum

REL

expression level of the studied gene relative to the expression of the reference gene

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • E. G. Varlamova
    • 1
  • M. V. Goltyaev
    • 1
  • J. P. Kuznetsova
    • 2
  1. 1.Institute of Cell BiophysicsRussian Academy of SciencesPushchinoRussia
  2. 2.Voronezh State University of Engineering TechnologiesVoronezhRussia

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