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Effect of Sodium Selenite on Gene Expression of SELF, SELW, and TGR Selenoproteins in Adenocarcinoma Cells of the Human Prostate

  • Molecular Cell Biology
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Abstract

Selenium is an essential trace element, the deficiency of which leads to the development of several serious diseases, including male infertility, prostate cancer, etc. It has been shown that oxidative stress contributes to the progression of prostate cancer, and antioxidants such as selenium and vitamin E can significantly reduce the risk of this disease. Sodium selenite, one of the selenium compounds that induce the formation of reactive oxygen species, is considered as a potential anticancer agent. The SS concentrations that lead to a decrease in the viability of human prostate adenocarcinoma cells (line Du-145) have been selected, and the effect of sodium selenite on the expression of mRNA of the SELV, SELW, and TGR selenocysteine proteins in these cells has been analyzed.

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Abbreviations

GAPDH:

glyceraldehyde-3-dehydrogenase

GPX:

glutathione peroxidase

IPTGP:

isopropyl β-D-1-thiogalactopyranoside

ORF:

open reading frame

ROS:

reactive oxygen species

Se:

selenium

Sec:

selenocysteine

SELV and SELW:

selenocysteine-containing proteins V and W

SS:

sodium selenite

TGR:

thioredoxin glutathione reductase

EPR:

endoplasmic reticulum

REL:

expression level of the studied gene relative to the expression of the reference gene

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Authors and Affiliations

  1. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    E. G. Varlamova & M. V. Goltyaev

  2. Voronezh State University of Engineering Technologies, Voronezh, 394036, Russia

    J. P. Kuznetsova

Authors
  1. E. G. Varlamova
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  2. M. V. Goltyaev
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  3. J. P. Kuznetsova
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Correspondence to E. G. Varlamova.

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Original Russian Text © E.G. Varlamova, M.V. Goltyaev, J.P. Kuznetsova, 2018, published in Molekulyarnaya Biologiya, 2018, Vol. 52, No. 3, pp. 519–526.

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Varlamova, E.G., Goltyaev, M.V. & Kuznetsova, J.P. Effect of Sodium Selenite on Gene Expression of SELF, SELW, and TGR Selenoproteins in Adenocarcinoma Cells of the Human Prostate. Mol Biol 52, 446–452 (2018). https://doi.org/10.1134/S0026893318030147

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  • DOI: https://doi.org/10.1134/S0026893318030147

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