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Comparative structural stability of subunits of the potato virus X coat protein in solution and in virus particles

  • Structural-Functional Analysis of Biopolymers and Their Complexes
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Abstract

Several optical methods and differential scanning calorimetry were used to study the structure and stability of free coat protein (CP) molecules and CP molecules in the virion of the potato virus X (PVX), a filamentous plant virus. All criteria suggest that PVX CP (hereinafter, CP) subunits in solution at room temperature display a certain preserved tertiary structure; however, this structure is very unstable and already denatures at 35°C. Very low concentrations of sodium dodecylsulfate or cetyltrimethylammonium bromide also disrupt the CP tertiary structure, three-five molecules of these detergents per one protein molecule being sufficient. However, the secondary structure of CP molecules does not change under the same conditions. Once included into the virion, CP subunits become considerably more stable towards increased temperature and detergents. This combination of a highly labile tertiary structure and a fairly stable secondary structure of free CP can be a structural basis for the recently discovered ability of PVX CP to assume two distinct functional states within the virion.

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Authors and Affiliations

  1. Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, 119992, Russia

    M. A. Nemykh

  2. Faculty of Biology, Moscow State University, Moscow, 119992, Russia

    V. K. Novikov

  3. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119992, Russia

    M. A. Nemykh, A. M. Arutyunyan, P. V. Kalmykov, V. A. Drachev & E. N. Dobrov

Authors
  1. M. A. Nemykh
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  2. V. K. Novikov
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  3. A. M. Arutyunyan
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  4. P. V. Kalmykov
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  5. V. A. Drachev
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  6. E. N. Dobrov
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Correspondence to E. N. Dobrov.

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Original Russian Text © M.A. Nemykh, V.K. Novikov, A.M. Arutyunyan, P.V. Kalmykov, V.A. Drachev, E.N. Dobrov, 2007, published in Molekulyarnaya Biologiya, 2007, Vol. 41, No. 4, pp. 697–705.

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Nemykh, M.A., Novikov, V.K., Arutyunyan, A.M. et al. Comparative structural stability of subunits of the potato virus X coat protein in solution and in virus particles. Mol Biol 41, 630–637 (2007). https://doi.org/10.1134/S0026893307040164

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  • DOI: https://doi.org/10.1134/S0026893307040164

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