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Methane Cycle in a Littoral Site of a Temperate Freshwater Lake

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Abstract—

Eutrophication of lakes results in the intensification of anaerobic processes, including methanogenesis, and therefore in enhanced emission of methane. A littoral area with its variable oxygen regime is the first to react to eutrophication. The diversity of microbial communities in littoral areas is insufficiently studied, and little data are available concerning the methane cycle microorganisms. In this work, the methanogenesis and methane oxidation were investigated in the littoral site of a freshwater temperate Lake Senezh (Russia). A combination of analytical, microbiological and molecular techniques was used, including physicochemical analyses, high-throughput sequencing, potential activity measurements, and cultivation on selective media. The littoral site was found to be an extremely labile ecological niche, which harbors a diverse community containing aerobic, facultative anaerobic and anaerobic microorganisms, both autotrophs and heterotrophs, which may perform all reactions of the N, S, and CH4 cycles. Methane formation was carried out via hydrogenotrophic, acetoclastic, methylotrophic, and methyl-reducing pathways. Among methanotrophs, type I organisms predominated; type II, nitrate- and nitrite-dependent methanotrophs were also revealed. Comparison of the average rates of methanogenesis and aerobic methane oxidation suggests that all methane, which may potentially be formed in the littoral site of the lake, could simultaneously be oxidized.

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ACKNOWLEDGMENTS

The authors are thankful to LLC “MGULAB” (https://www.msulab.ru/) for the chemical analysis of water samples and to Prof. N.V. Ravin (Research Centre of Biotechnology RAS) for critical reading of the manuscript and useful recommendations.

Funding

This research was partially funded by Russian Science Foundation, grant no. 22-14-00038 (activity measurements, enrichments isolation, sequencing of native samples and methanogenic enrichments). Sequencing of the methanotrophic enrichment and bioinformatic analyses were funded by Russian Foundation for Basic Research according to the research project no. 20-04-60190. Field work was supported by State Assignment for the Laboratory of relict microbial communities, Research Centre of Biotechnology RAS.

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Authors and Affiliations

  1. Research Centre of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia

    A. Yu. Kallistova, D. D. Koval, V. V. Kadnikov, S. K. Yusupov & N. V. Pimenov

  2. Kurchatov Center for Genome Research, National Research Center “Kurchatov Institute”, 123098, Moscow, Russia

    S. V. Toshchakov, A. O. Izotova & E. N. Vinogradova

  3. Faculty of Biology, Moscow State University, 119991, Moscow, Russia

    E. N. Vinogradova

  4. Faculty of Science, Institute of Chemistry, University of Tartu, 50411, Tartu, Estonia

    I. Zekker

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  1. A. Yu. Kallistova
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  2. D. D. Koval
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  3. V. V. Kadnikov
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  4. S. V. Toshchakov
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  5. S. K. Yusupov
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  6. A. O. Izotova
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  7. E. N. Vinogradova
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  9. N. V. Pimenov
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Correspondence to A. Yu. Kallistova.

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This article does not contain any studies involving animals or human participants performed by any of the authors. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Kallistova, A.Y., Koval, D.D., Kadnikov, V.V. et al. Methane Cycle in a Littoral Site of a Temperate Freshwater Lake. Microbiology 92, 153–170 (2023). https://doi.org/10.1134/S0026261722602901

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  • DOI: https://doi.org/10.1134/S0026261722602901

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