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Effect of Carbon Nanoparticles with Different Structural Organization on the Biological Systems of Escherichia coli K12 TGI

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Abstract—

The onset of large-scale industrial and household application of nanoobjects has boosted interest in research of interactions between nanoparticles of various nature and biological objects. However, the impact of nanomaterials on biological systems, including humans, is not fully understood. The results obtained recently in various laboratories do not give an accurate answer about their safety. Previously, we found that exposure of bacterial cells to single- and multi-walled carbon nanotubes resulted in their decay. To explain this effect, in this work we analyzed the effect of two other types of carbon nanoparticles, diamond and graphite, on bacterial cells. Engineered cells of Escherichia coli strain K12 TGI with a luminous phenotype were used as a test system to study the effects of diamond nanoparticles obtained by the detonation method and of graphite nanoparticles obtained by sonication. Based on bioluminescence and scanning electron microscopy data, it was shown that these types of nanoparticles were not toxic to bacterial cells. Our results demonstrate that the safety of nanomaterials depends not only on their composition, but also on their internal structure. This will help to further develop safety criteria for nanomaterials and to establish the conditions and standards for their use.

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ACKNOWLEDGMENTS

The authors are especially grateful to A.P. Zarubina for assistance in carrying out this study and for valuable advice.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    E. V. Sorokina

  2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

    E. A. Obraztsova

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  1. E. V. Sorokina
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  2. E. A. Obraztsova
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Correspondence to E. V. Sorokina.

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Statement on the welfare of animals. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by A. Panyushkina

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Sorokina, E.V., Obraztsova, E.A. Effect of Carbon Nanoparticles with Different Structural Organization on the Biological Systems of Escherichia coli K12 TGI. Microbiology 91, 286–291 (2022). https://doi.org/10.1134/S0026261722300075

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