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Evaluation of the diversity of nitrogen-fixing bacteria in soybean rhizosphere by nifH gene analysis

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Abstract

Biological nitrogen fixation plays an important role in the nitrogen balance of agricultural ecosystems and provides an essential part of nitrogen nutrition for plants, even in conditions of intensive fertilization. The main agrobiotechnological method for soybean cultivation (Glycine max (L.) Merril) is an application of microbial preparations based on Bradyrhizobium japonicum. Successful inoculation strongly depends on the interactions between the introduced microorganism and the aboriginal rhizosphere microorganisms. To evaluate the composition of diazotrophic communities, a study of the diversity of the molecular marker for nitrogen fixation, the nifH gene, in the samples of soybean rhizosphere soil was carried out. Experiments were performed in the variants when soybean was cultivated without inoculation and after adding bacterial preparations, as well as in enrichment cultures of diazotrophs. The revealed diazotrophic microorganisms demonstrated low level of similarity to the known microorganisms (74–95% identity by nucleotides), and were identified as species of the phyla Firmicutes and Proteobacteria. In the composition of nitrogen-fixing communities in the rhizosphere soil, the microorganisms of the genera Clostridium, Paenibacillus, and Spirochaeta were shown to prevail.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia

    A. K. Kizilova & I. K. Kravchenko

  2. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, Ukraine

    L. V. Titova & G. A. Iutinskaya

Authors
  1. A. K. Kizilova
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  2. L. V. Titova
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  3. I. K. Kravchenko
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  4. G. A. Iutinskaya
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Correspondence to A. K. Kizilova.

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Original Russian Text © A.K. Kizilova, L.V. Titova, I.K. Kravchenko, G.A Iutinskaya, 2012, published in Mikrobiologiya, 2012, Vol. 81, No. 5, pp. 672–681.

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Kizilova, A.K., Titova, L.V., Kravchenko, I.K. et al. Evaluation of the diversity of nitrogen-fixing bacteria in soybean rhizosphere by nifH gene analysis. Microbiology 81, 621–629 (2012). https://doi.org/10.1134/S0026261712050116

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  • DOI: https://doi.org/10.1134/S0026261712050116

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