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An analysis of the chlorophyll fluorescence transient by spectral multi-exponential approximation

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Abstract

A method to analyze the chlorophyll fluorescence transient was developed using approximation of the experimental signal by a multi-exponential series. Visualization of partial sums of the series made it possible to find the amplitudes and characteristic times for individual phases of the fluorescence induction curve. The method gave more rigid criteria of phase identification as compared with the current semi-empirical approach. Applied to the Chlamidomonas reinhardtii sulfur deprivation case, the method was efficient in finding visually indistinguishable phases of the fluorescence transient, thus allowing early detection of stress.

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Authors and Affiliations

  1. Department of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia

    T. Yu. Plyusnina, S. S. Khruschev, G. Yu. Riznichenko & A. B. Rubin

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  1. T. Yu. Plyusnina
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  2. S. S. Khruschev
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  3. G. Yu. Riznichenko
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  4. A. B. Rubin
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Correspondence to T. Yu. Plyusnina.

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Original Russian Text © T.Yu. Plyusnina, S.S. Khruschev, G.Yu. Riznichenko, A.B. Rubin, 2015, published in Biofizika, 2015, Vol. 60, No. 3, pp. 487–495.

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Plyusnina, T.Y., Khruschev, S.S., Riznichenko, G.Y. et al. An analysis of the chlorophyll fluorescence transient by spectral multi-exponential approximation. BIOPHYSICS 60, 392–399 (2015). https://doi.org/10.1134/S000635091503015X

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  • DOI: https://doi.org/10.1134/S000635091503015X

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