Skip to main content
SpringerLink
Log in

In vivo analysis of chlorophyll a fluorescence induction

  • Regular Paper
  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

Quantitative characteristics of photosynthetic electron transport were evaluated in vivo on the basis of the multi-exponential analysis of OJIP fluorescence transients induced by saturating actinic light. The OJIP fluorescence curve F(t), measured in Chlamydomonas reinhardtii cells, was transformed into the (1 − F O/F(t)) × (F V /F M)−1 transient, which is shown to relate to PS 2 closure. We assumed that kinetics of PS 2 closure during OJIP rise reflects time-separated processes related to the establishment of redox equilibrium at the PS 2 acceptor side (OJ), PQ pool (JI), and beyond Cyt b/f (IP). Three-exponential fitting was applied to (1 − F O/F(t)) × (F V /F M)−1 transient to obtain lifetimes and amplitudes of the OJ, JI, and IP components of PS 2 closure, which were used to calculate overall rates of reduction and re-oxidation of the PS 2 acceptor side, PQ pool, and intermediates beyond Cyt b/f complex. The results, obtained in the presence of inhibitors, oxidative reagents, and under different stress conditions prove the suggested model and characterize the introduced parameters as useful indicators of photosynthetic function.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

Chl:

Chlorophyll

PS:

Photosystem

QA :

The primary quinone electron acceptor in PS 2

QB :

The secondary quinone electron acceptor in PS 2

PQ:

Plastoquinone

IPI:

Electron carriers between cytochrome b6/f and ferredoxin-NADP reductase (IP phase related intermediates)

Fd:

Ferredoxin

FNR:

Ferredoxin-NADP reductase

P700 :

Reaction center pigment of photosystem 1

PC:

Plastocyanin

Cyt b/f:

Cytochrome b6/f complex

DCMU:

3-(3,4-Dichlorophenyl)-1,1-dimethylurea

DBMIB:

Dibromothymoquinone, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone

MV:

Methylviologen

FeCy:

Ferricyanide

S:

Sulfur

O, J, I, P:

Particular steps in light-induced Chl fluorescence curve

References

  • Antal TK, Graevskaya EE, Matorin DN, Voronova EN, Pogosyan SI, Rubin AB (2004) Fluorescence study of the effect of mercuric chloride and methylmercury on photosynthetic activity of the diatom Thalassiosira weissflogii. Biofizika 49:72–78

    PubMed  CAS  Google Scholar 

  • Antal TK, Volgusheva AA, Kukarskih GP, Bulychev AA, Krendeleva TE, Rubin AB (2006) Effects of sulfur limitation on the PS II functioning in Chlamydomonas reinhardtii as probed by the chlorophyll a fluorescence. Physiol Plantarum 128:360–367

    Article  CAS  Google Scholar 

  • Antal TK, Krendeleva TE, Rubin AB (2007) Study of photosystem 2 heterogeneity in the sulfur-deficient green alga Chlamydomonas reinhardtii. Photosynth Res 94:13–22

    Article  PubMed  CAS  Google Scholar 

  • Boisvert S, Joly D, Carpentier R (2006) Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step. FEBS J 273:4770–4777

    Article  PubMed  CAS  Google Scholar 

  • Bukhov N, Egorova E, Govindachary S, Carpentier R (2004) Changes in polyphasic chlorophyll a fluorescence induction curve upon inhibition of donor or acceptor side of photosystem II in isolated thylakoids. Biochim Biophys Acta 1657:121–130

    Article  PubMed  CAS  Google Scholar 

  • Duysens LNM and Sweers HE (1963) Mechanism of two photochemical reactions in algae as studied by means of fluorescence. In: Japanese Society of Plant Physiologists (eds) Studies on microalgae and photosynthetic bacteria. University of Tokyo Press, Tokyo, pp 353–372

  • French KJ, Scruton DA, Anderson MR, Schneider DC (2004) Influence of physical and chemical characteristics on mercury in aquatic sediments. Water Air Soil Pollut 110:346–362

    Google Scholar 

  • Haehnel W (1984) Photosynthetic electron transport in higher plants. Ann Rev Plant Physiol 35:659–693

    CAS  Google Scholar 

  • Kukarskikh GL, Graevskaia EE, Krendeleva TE, Timofeev KN, Rubin AB (2003) Effect of methylmercury on primary photosynthesis processes in green microalgae Chlamydomonas reinhardtii. Biofizika 48:853–859

    PubMed  CAS  Google Scholar 

  • Lazár D (1999) Chlorophyll a fluorescence induction. Biochim Biophys Acta 1412:1–28

    Article  PubMed  Google Scholar 

  • Laźar D (2003) Chlorophyll a fluorescence rise induced by high light illumination of dark-adapted plant tissue studied by means of a model of photosystem II and considering photosystem II heterogeneity. J Theor Biol 220:469–503

    Article  PubMed  Google Scholar 

  • Lazár D, Pospìšil P (1999) Mathematical simulation of chlorophyll a fluorescence rise measured with 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea-treated barley leaves at room and high temperatures. Eur Biophys J 28:468–477

    Article  PubMed  Google Scholar 

  • Melis A, Homann PH (1975) Kinetic analysis of the fluorescence induction in 3-(3,4-dichlorophenyl)-1,1-dimethylurea poisoned chloroplasts. Photochem Photobiol 21:431–437

    Article  CAS  Google Scholar 

  • Melis A, Zhang L, Forestier M, Ghirardi M, Seibert M (2000) Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol 122:127–136

    Article  PubMed  CAS  Google Scholar 

  • Moreland DE (1980) Mechanisms of action of herbicides. Annu Rev Plant Physiol 31:597–638

    Article  CAS  Google Scholar 

  • Paillotin G (1976) Movement of excitations in the photosynthetic domains of photosystem II. J Theor Biol 58:237–252

    Article  CAS  Google Scholar 

  • Pätsikkä E, Aro E-M, Tyystjärvi E (1998) Increase in the quantum yield of photoinhibition contributes to copper toxicity in vivo. Plant Physiol 117:619–627

    Article  PubMed  Google Scholar 

  • Pospìšil P, Dau H (2002) Valinomycin sensitivity proves that light-induced thylakoid voltages result in millisecond phase of chlorophyll fluorescence transients. Biochim Biophys Acta 1554:94–100

    Article  PubMed  Google Scholar 

  • Sager R, Granick S (1953) Nutritional studies with Chlamydomonas reinhardtii. Ann NY Acad Sci 56:831–838

    Article  PubMed  CAS  Google Scholar 

  • Schansker G, Srivastava A, Govindjee, Strasser R (2003) Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves. Funct Plant Biol 30:785–796

    Article  CAS  Google Scholar 

  • Schansker G, Tóth SZ, Strasser RJ (2005) Methylviologen and dibromothymoquinone treatments of pea leaves reveal the role of photosystem I in the Chl a fluorescence rise OJIP. Biochim Biophys Acta 1706:250–261

    Article  PubMed  CAS  Google Scholar 

  • Schansker G, Tóth SZ, Strasser RJ (2006) Dark recovery of the Chl a fluorescence transient (OJIP) after light adaptation: the qT-component of non-photochemical quenching is related to an activated photosystem I acceptor side. Biochim Biophys Acta 1757:787–797

    Article  PubMed  CAS  Google Scholar 

  • Schmitz P, Maldonado-Rodriguez R, Strasser RJ (2001) Evaluation of the nodulated status of Vigna unguiculata probed by the JIP-test based on the chlorophyll a fluorescence rise. In: Proceedings of the 12th international congress on photosynthesis, S36-012, CSIRO Publishing, Melbourne (CD-ROM)

  • Stirbet A, Govindjee, Strasser BJ, Strasser RJ (1998) Chlorophyll a fluorescence induction in higher plants: modelling and numerical simulation. J Theor Biol 193:131–151

    Article  CAS  Google Scholar 

  • Strasser BJ (1997) Donor side capacity of photosystem II probed by chlorophyll a fluorescence transients. Photosynth Res 52:147–155

    Article  CAS  Google Scholar 

  • Strasser RJ, Govindjee (1992) The FO and the O-J-I-P fluorescence rise in higher plants and algae. In: Argyroudi-Akoyunoglou JH (ed) Regulation of chloroplast biogenesis. Plenum Press, New York, pp. 423–426

    Google Scholar 

  • Strasser BJ, Strasser RJ (1995) Measuring fast fluorescence transients to address environmental questions: the JIP-test. In: Mathis P (ed) Photosynthesis: from light to biosphere. Kluwer Academic Publishers, Dordrecht, pp 977–980

    Google Scholar 

  • Strasser RJ, Tsimilli-Michael M, Srivastava A (2005) Analysis of chlorophyll a fluorescence transient. In: Papageorgiou GC, Govindjee (eds) Chlorophyll fluorescence: a signature of photosynthesis. Kluwer Academic Publishers, Dordrecht/Boston/London, pp 321–362

    Google Scholar 

  • Talts E, Oja V, Rämma H, Rasulov B, Anijalg A, Laisk A (2007) Dark inactivation of ferredoxin-NADP reductase and cyclic electron flow under far-red light in sunflower leaves. Photosynth Res 94:109–120

    Article  PubMed  CAS  Google Scholar 

  • Vavilin DV, Polynov VA, Matorin DN, Venediktov PS (1995) Sublethal concentrations of copper stimulate photosystem II photoinhibition in Chlorella pyrenoidosa. J Plant Physiol 146:609–614

    CAS  Google Scholar 

  • Vernotte C, Etienne AL, Briantais J-M (1979) Quenching of the system II chlorophyll fluorescence by the plastoquinone pool. Biochim Biophys Acta 545:519–527

    Article  PubMed  CAS  Google Scholar 

  • Volgusheva AA, Zagidullin VE, Antal TK, Krendeleva TE, Paschenko VZ, Rubin AB (2007) Examination of chlorophyll fluorescence decay kinetics in sulfur deprived algae Chlamydomonas reinhardtii. Biochim Biophys Acta 1767:559–564

    Article  PubMed  CAS  Google Scholar 

  • Vredenberg WJ, Bulychev A (2003) Photoelectric effects on chlorophyll fluorescence of photosystem II in vivo. Kinetics in the absence and presence of valinomycin. Bioelectrochem 60:87–95

    Article  CAS  Google Scholar 

  • Wykoff DD, Davies JP, Melis A, Grossman A (1998) The effects of phosphorus and sulfur deprivation on photosynthetic electron transport in Chlamydomonas reinhardtii. Plant Physiol 117:129–139

    Article  PubMed  CAS  Google Scholar 

  • Zhang L, Happe T, Melis A (2002) Biochemical and morphological characterization of sulfur-deprived and H2-producing Chlamydomonas reinhardtii (green alga). Planta 214:552–561

    Article  PubMed  CAS  Google Scholar 

  • Zhu X-G, Govindjee, Baker NR, de Sturler E, Ort DR, Long SP (2005) Chlorophyll a fluorescence induction kinetics in leaves predicted from a model describing each discrete step of excitation energy and electron transfer associated with photosystem II. Planta 223:114–133

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful to Profs. T. Krendeleva, G. Riznichenko, D. Matorin, and E. Tyystjärvi, as well as to Drs. A. Volgusheva, I. Kovalenko, and E. Graevskaya for the assistance in experimental work, providing their experimental data for this analysis, and fruitful discussions. This work was supported by the Russian Foundation of Basic Research (07-04-00222) and the U.S. Civilian Research & Development Foundation (CRDF) (10841).

Author information

Authors and Affiliations

  1. Biological Faculty, Moscow State University, Vorobyovi Gory, 119992, Moscow, Russia

    T. Antal & A. Rubin

Authors
  1. T. Antal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Rubin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. Antal.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Antal, T., Rubin, A. In vivo analysis of chlorophyll a fluorescence induction. Photosynth Res 96, 217–226 (2008). https://doi.org/10.1007/s11120-008-9301-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11120-008-9301-6

Keywords

Search

Navigation