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Effects of fibroin microcarriers on inflammation and regeneration of deep skin wounds in mice

  • Molecular and Cellular Mechanisms of Inflammation (Special Issue) Guest Editors S. A. Nedospasov and D. V. Kuprash
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Abstract

The process of tissue regeneration following damage takes place with direct participation of the immune system. The use of biomaterials as scaffolds to facilitate healing of skin wounds is a new and interesting area of regenerative medicine and biomedical research. In many ways, the regenerative potential of biological material is related to its ability to modulate the inflammatory response. At the same time, all foreign materials, once implanted into a living tissue, to varying degree cause an immune reaction. The modern approach to the development of bioengineered structures for applications in regenerative medicine should be directed toward using the properties of the inflammatory response that improve healing, but do not lead to negative chronic manifestations. In this work, we studied the effect of microcarriers comprised of either fibroin or fibroin supplemented with gelatin on the dynamics of the healing, as well as inflammation, during regeneration of deep skin wounds in mice. We found that subcutaneous administration of microcarriers to the wound area resulted in uniform contraction of the wounds in mice in our experimental model, and microcarrier particles induced the infiltration of immune cells. This was associated with increased expression of proinflammatory cytokines TNF, IL-6, IL-1β, and chemokines CXCL1 and CXCL2, which contributed to full functional recovery of the injured area and the absence of fibrosis as compared to the control group.

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Abbreviations

CXCL1(2):

CXC-chemokine ligand 1(2)

F:

fibroin

FG:

fibroin-gelatin

FG-MC:

fibroin microcarriers supplemented with gelatin

F-MC:

fibroin microcarriers

IL1ß:

interleukin 1ß

IL-6:

interleukin 6

PBS:

phosphate buffered saline

TNF:

tumor necrosis factor

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Authors and Affiliations

  1. Biological Faculty, Lomonosov Moscow State University, 119991, Moscow, Russia

    A. Y. Arkhipova, M. A. Nosenko, N. V. Malyuchenko, A. S. Zhdanova, T. V. Vasil’eva, E. A. Gorshkova, M. S. Drutskaya, S. A. Nedospasov & M. M. Moisenovich

  2. Engelhardt Institute of Molecular Biology, 119991, Moscow, Russia

    M. A. Nosenko, R. V. Zvartsev, A. M. Moisenovich, A. S. Zhdanova, E. A. Gorshkova, M. S. Drutskaya & S. A. Nedospasov

  3. Shumakov Research Institute of Transplantation and Artificial Organs, Russian Ministry of Health, 113182, Moscow, Russia

    I. I. Agapov

  4. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    S. A. Nedospasov

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  1. A. Y. Arkhipova
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  2. M. A. Nosenko
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  3. N. V. Malyuchenko
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  4. R. V. Zvartsev
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  5. A. M. Moisenovich
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  6. A. S. Zhdanova
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  7. T. V. Vasil’eva
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  8. E. A. Gorshkova
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  9. I. I. Agapov
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  10. M. S. Drutskaya
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  11. S. A. Nedospasov
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  12. M. M. Moisenovich
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Corresponding author

Correspondence to M. S. Drutskaya.

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These authors contributed equally to this work.

Published in Russian in Biokhimiya, 2016, Vol. 81, No. 11, pp. 1494–1504.

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Arkhipova, A.Y., Nosenko, M.A., Malyuchenko, N.V. et al. Effects of fibroin microcarriers on inflammation and regeneration of deep skin wounds in mice. Biochemistry Moscow 81, 1251–1260 (2016). https://doi.org/10.1134/S0006297916110031

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  • DOI: https://doi.org/10.1134/S0006297916110031

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