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Trigger factor assists the refolding of heterodimeric but not monomeric luciferases

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Abstract

The refolding of thermally inactivated protein by ATP-independent trigger factor (TF) and ATP-dependent DnaKJE chaperones was comparatively analyzed. Heterodimeric (αβ) bacterial luciferases of Aliivibrio fischeri, Photobacterium leiognathi, and Vibrio harveyi as well as monomeric luciferases of Vibrio harveyi and Luciola mingrelica (firefly) were used as substrates. In the presence of TF, thermally inactivated heterodimeric bacterial luciferases refold, while monomeric luciferases do not refold. These observations were made both in vivo (Escherichia coli ΔdnaKJ containing plasmids with tig gene) and in vitro (purified TF). Unlike TF, the DnaKJE chaperone system refolds both monomeric and heterodimeric luciferases with equal efficiency.

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Abbreviations

DnaKJE:

chaperone

DnaK:

DnaJ-GrpE

TF:

trigger factor

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Authors and Affiliations

  1. Research Institute for Genetics and Selection of Industrial Microorganisms (GosNIIGenetika), 1-yi Dorozhnyi Proezd 1, 117545, Moscow, Russia

    O. E. Melkina, I. I. Goryanin, I. V. Manukhov & G. B. Zavilgelsky

  2. School of Systems Biology, College of Science, George Mason University, Fairfax, VA, 22003, USA

    A. V. Baranova

  3. Institute of Protein Research, Russian Academy of Sciences, ul. Institutskaya 4, 142290, Pushchino, Moscow Region, Russia

    V. A. Kolb & M. S. Svetlov

Authors
  1. O. E. Melkina
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  2. I. I. Goryanin
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  3. I. V. Manukhov
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  4. A. V. Baranova
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  5. V. A. Kolb
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  6. M. S. Svetlov
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  7. G. B. Zavilgelsky
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Corresponding author

Correspondence to G. B. Zavilgelsky.

Additional information

Published in Russian in Biokhimiya, 2014, Vol. 79, No. 1, pp. 79–86.

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Melkina, O.E., Goryanin, I.I., Manukhov, I.V. et al. Trigger factor assists the refolding of heterodimeric but not monomeric luciferases. Biochemistry Moscow 79, 62–68 (2014). https://doi.org/10.1134/S000629791401009X

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  • DOI: https://doi.org/10.1134/S000629791401009X

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