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Putative GalP, XylE, and FucP H+ symporters from Pantoea ananatis are capable of transporting glucose into Escherichia coli cells

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Abstract

The involvement of presumably low-affinity H+ symporter GalP in the glucose uptake by Pantoea ananatis cells was demonstrated. The putative galP, xylE, and fucP genes from P. ananatis AJ13355—orthologs of the known E. coli genes for H+ symporters of D-galactose, D-xylose, and L-fucose, respectively, were cloned. It was confirmed that the constitutive expression of each of the cloned genes restored the deleted E. coli MG1655Δ(ptsHI-crr) strain growth on D-glucose. The constructed integrative cassettes, providing the constitutive expression of the galP, xylE, and fucP genes from P. ananatis, could be used for the optimization of glucose consumption in producing strains based on P. ananatis or E. coli.

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Abbreviations

VKPM:

All-Russia Collection of Industrial Microorganisms, Research Institute of Genetics (GosNIIgenetika), Moscow

OD:

optical density

PCR:

polymerase chain reaction

LB medium:

Luria-Bertani medium

CRP:

catabolic repressor protein

GalP,XylE, and FucP:

low-affinity H+ symporters of D-galactose, D-xylose, and L-fucose, respectively

G6P:

glucose 6-phosphate

mGDH:

membrane-bound glucose dehydrogenase

ORF:

open reading frame

PEP:

phosphoenolpyruvate

PTS:

phosphoenolpyruvate-dependent phosphotransferase system

PQQ:

pyrrolequinoline quinone

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Authors and Affiliations

  1. Ajinomoto-Genetika Research Institute, Moscow, 117545, Russia

    I. G. Andreeva, L. I. Golubeva & J. I. Katashkina

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  1. I. G. Andreeva
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  2. L. I. Golubeva
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  3. J. I. Katashkina
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Correspondence to I. G. Andreeva.

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Original Russian Text © I.G. Andreeva, L.I. Golubeva, J.I. Katashkina, 2012, published in Biotekhnologiya, 2012, No. 2, pp. 21–31.

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Andreeva, I.G., Golubeva, L.I. & Katashkina, J.I. Putative GalP, XylE, and FucP H+ symporters from Pantoea ananatis are capable of transporting glucose into Escherichia coli cells. Appl Biochem Microbiol 49, 638–645 (2013). https://doi.org/10.1134/S0003683813070016

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  • DOI: https://doi.org/10.1134/S0003683813070016

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