Abstract
This review analyzes the methodology available for the creation and analysis of metagenomic libraries, targeted search for genes encoding certain types of enzymes, and also phenotypic selection of clones containing functioning genes with consequent identification by sequencing, cloning, and heterological expression in surrogate hosts in order to study gene products. Special attention was paid to the description of new enzyme families (lipases, esterases, oxidases, cellulases, proteases, and others) that are characterized by valuable biotechnological properties, such as thermostability, resistance to cold, alkaline pH, organic solvents, salinity, xenobiotics and multifunctionality, substrate specificity, and stereoselectivity.
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Abbreviations
- IBCh:
-
bacterial artificial chromosome
- PCR:
-
polymerase chain reaction
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Original Russian Text © S.V. Shestakov, 2011, published in Biotekhnologiya, 2011, No. 6, pp. 8–22.
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Shestakov, S.V. Impact of metagenomics on biotechnological development. Appl Biochem Microbiol 48, 705–715 (2012). https://doi.org/10.1134/S0003683812090050
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DOI: https://doi.org/10.1134/S0003683812090050