Skip to main content
SpringerLink
Log in

Stability of the rolC gene and its expression in 15-year-old cell cultures of Panax ginseng

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

After 15 years of cultivation of Panax ginseng, transgenic cell cultures were shown to express rolC gene at a high level. We determined that the rolC gene underwent mutagenesis. In particular, from one to four nucleotides were changed for the whole gene sequence (540 bp). These substitutions were synonymous in half of the cases or resulted in the modification of single amino acids in the rolC-encoded protein. With the example of β-1,3-glucanases we showed that long cultivation and the observed changes in nucleotide sequences of the transgene did not inhibit the activating effect of rolC on enzymatic activity of β-1,3-glucanases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Dicosmo, F. and Misawa, M., Biotch. Adv., 1995, vol. 13, no. 3, pp. 425–453.

    Article  CAS  Google Scholar 

  2. Cardarelli, M., Mariotti, D., Pomponi, M., Spano, L., Carone, I., and Costantino, P., Mol. Gen. Genet., 1987, vol. 209, no. 3, pp. 475–480.

    Article  PubMed  CAS  Google Scholar 

  3. Schmullign, T., Shell, J., and Spena, A., EMBO J., 1988, vol. 7, no. 9, pp. 2621–2629.

    Google Scholar 

  4. Spena, A., Schmullign, T., Koncz, C., and Schell, J.S., EMBO J., 1988, vol. 7, no. 9, pp. 2621–2629.

    PubMed  Google Scholar 

  5. Bonhomme, V., Laurain-Mattar, D., Lacoux, J., Fliniaux, M., and Jacquin-Dubreuil, A., J. Biotechnol., 2000, vol. 81, nos. 2–3, pp. 151–158.

    Article  PubMed  CAS  Google Scholar 

  6. Palazon, J., Cusidy, R.M., Roig, C., and Picol, M.T., Plant. Physiol. Biochem., 1997, vol. 35, no. 2, pp. 155–162.

    CAS  Google Scholar 

  7. Bulgakov, V.P., Khodakovskaya, M.V., Labetskaya, N.V., Chernoded, G.K., and Zhruavlev, Y.N., Phytochemistry, 1998, vol. 49, no. 7, pp. 1926–1934.

    Article  Google Scholar 

  8. Bulgakov, V.P., Kusaykin, M., Tchernoded, G.K., Zvyagintseva, T.N., and Zhruavlev, Y.N., Fitoterapia, 2002, vol. 73, nos. 7–8, pp. 638–643.

    Article  PubMed  CAS  Google Scholar 

  9. Kiselev, K.V., Kusaykin, M., Dubrovina, A.S., Bezvebny, D.A., Zvyagintseva, A.T., and Bulgakov, V.P., Phytochemistry, 2006, vol. 67, no. 20, pp. 2225–2231.

    Article  PubMed  CAS  Google Scholar 

  10. Kiselev, K.V., Dubrovina, A.S., Veselova, M.V., Bulgakov, V.P., Fedoreyev, S.A., and Zhuravlev, Y.N., J. Biotechnol., 2007, vol. 128, no. 3, pp. 681–692.

    Article  PubMed  CAS  Google Scholar 

  11. Zdrakovic-Korac, S., Calic, D., Druart, P.H., and Radojevic, L., Biol. Plant., 2003, vol. 47, no. 3, pp. 487–491.

    Google Scholar 

  12. Peebles, C.A., Gibson, S.I., Shanks, J.V., and San, K.Y., Biotechnol. Prog., 2007, vol. 23, no. 5, pp. 1517–1518.

    Article  PubMed  CAS  Google Scholar 

  13. Gorpenchenko, T.Y., Kiselev, K.V., Bulgakov, V.P., Tchernoded, G.K., Bragina, E.A., Khodakovskaya, M.V., Koren, O.G., Batygina, T.B., and Zhuravlev, Y.N., Planta, 2006, vol. 223, no. 3, pp. 457–467.

    Article  PubMed  CAS  Google Scholar 

  14. Kiselev, K.V., Gorpenchenko, T.Yu., Tchernoded, G.K., Dubrovina, A.S., Grishchenko, O.V., Bulgakov, V.P., and Zhuravlev, Yu.N., Mol. Biol. (Moscow), 2008, vol. 42, no. 2, pp. 243–252.

    Article  CAS  Google Scholar 

  15. Echt, C.S., Erdahl, L.A., and McCoy, T.J., Genome, vol. 35, no. 1, pp. 84–87.

  16. Bulgakov, V.P., Veselova, M.V., Tchernoded, G.K., Kiselev, K.V., Fedoreyev, S.A., and Zhuravlev, Y.N., Planta, 2005, vol. 221, no. 4, pp. 471–478.

    Article  PubMed  CAS  Google Scholar 

  17. Ding, J., Jia, J., Yang, L., Wen, H., Zhang, C., Jia, J., Weng, H., Liu, W., and Zhang, D., J. Agric. Food Chem., 2004, vol. 52, no. 11, pp. 3372–3377.

    Article  PubMed  CAS  Google Scholar 

  18. Yang, L., Ding, J., Zhang, C., Jia, J., Weng, H., Liu, W., and Zhang, D., Plant Cell Rep., 2005, vol. 23, nos. 10–11, pp. 759–763.

    Article  PubMed  CAS  Google Scholar 

  19. Vaucheret, H., Beclin, C., Elmayan, T., Feuerbach, F., Godon, C., Morel, J.B., Mourrain, P., Palauqui, J.C., and Vernhettes, S., Plant J., 1998, vol. 16, no. 6, pp. 651–659.

    Article  PubMed  CAS  Google Scholar 

  20. Dieguez, M.J., Vaucheret, H., Paszkowski, J., and Scheid, O.M., Mol. Gen. Genet., 1998, vol. 259, no. 2, pp. 207–215.

    Article  PubMed  CAS  Google Scholar 

  21. Aoki, S. and Syono, K., Proc. Natl. Acad. Sci. USA, 1999, vol. 96, no. 23, pp. 13229–13234.

    Article  PubMed  CAS  Google Scholar 

  22. Intrieri, M.C. and Buiatti, M., Mol. Phylogemet. Evol., 2001, vol. 20, no. 1, pp. 100–110.

    Article  CAS  Google Scholar 

  23. Goodman, M., Prog. Biophys. Mol. Biol., 1981, vol. 38, no. 2, pp. 105–164.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biology and Soil Science, Far East Division, Russian Academy of Sciences, Vladivostok, 690022, Russia

    K. V. Kiselev & V. P. Bulgakov

Authors
  1. K. V. Kiselev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. P. Bulgakov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. V. Kiselev.

Additional information

Original Russian Text © K.V. Kiselev, V.P. Bulgakov, 2009, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2009, Vol. 45, No. 5, pp. 618–624.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kiselev, K.V., Bulgakov, V.P. Stability of the rolC gene and its expression in 15-year-old cell cultures of Panax ginseng . Appl Biochem Microbiol 45, 555–561 (2009). https://doi.org/10.1134/S0003683809050172

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683809050172

Keywords

Search

Navigation