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Plant regeneration from rice anthers cryopreserved by an encapsulation/dehydration technique

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Summary

Anther-derived rice (Oryza sativa L. ssp. japonica variety Yerua P.A.) plants were obtained after cryopreservation by an encapsulation/dehydration technique. Immature anthers, excised from spikelets pretreated at 8°C for 8d, were encapsulated in calcium alginate beads. The beads were cultured on N6 medium with 11.5 μM naphthalenaecetic acid (NAA) and 2.3 μM 6-furfurylaminopurine (KIN). Fifteen percent of the encapsulated anthers formed calluses when pretreated with sucrose for 3 d in liquid medium, desiccated on silica gel, slowly cooled to −30°C, immersed in liquid nitrogen (LN), thawed, and recultured. The cryopreserved encapsulated anthers produced 1.67 shoots/callus, in contrast to the control (non-cooled encapsulated anthers), which produced 6 shoots/callus. Eighty percent of the plantlets developed into normal plants after being transferred to greenhouse conditions. Histological observations showed that the origin of the plants was not modified by the cryopreservation process.

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Authors and Affiliations

  1. Instituto de Botánica del Nordeste-Facultad de Ciencias Agrarias (UNNE), Casilla de Correo 209, 3400, Corrientes, Argentina

    María A. Marassi, Adriana Scocchi & Ana María Gonzalez

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  1. María A. Marassi
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  2. Adriana Scocchi
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  3. Ana María Gonzalez
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Correspondence to María A. Marassi.

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Marassi, M.A., Scocchi, A. & Gonzalez, A.M. Plant regeneration from rice anthers cryopreserved by an encapsulation/dehydration technique. In Vitro Cell.Dev.Biol.-Plant 42, 31–36 (2006). https://doi.org/10.1079/IVP2005746

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  • DOI: https://doi.org/10.1079/IVP2005746

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