Summary
Black grama (Bouteloua eriopoda) is an important forage grass in southwestern USA rangelands. Plants were regenerated by somatic embryogenesis. Surface-disinfested seeds were germinated and the embryonic shoots were excised and cultured on Murashige and Skoog (MS) medium gelled with agar. Callus was induced from apical meristems. Calluses were cultured on MS solid medium with six concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) or Dicamba (6-dichloro-o-anisic acid) for 6 wk under light or dark conditions. Somatic embryo induction was greatest on 4.52 μM Dicamba, under light, after transferring to an auxin-free medium. Embryo development progressed from globular torpedo to mature embryos phenotypically identical to those naturally produced in seed. These germinated and grew into intact plants and were established in soil and grown to maturity. To our knowledge, this is the first report of somatic embryo induction and regeneration in black grama grass.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aguado-Santacruz, G. A.; Cabrera-Ponce, J. L.; Olalde-Portugal, V.; Sanchez-Gonzalez, M. R.; Marquez-Guzman, M. R.; Herrera-Estrella, L. Tissue culture and plant regeneration of blue grama grass Bouteloua gracilis (H.B.K.) Lag. ex Steud. In Vitro Cell. Dev. Biol. Plant 37:182–189; 2001a.
Aguado-Santacruz, G. A.; Cabrera-Ponce, J. L.; Ramirez-Chavez, E.; Leon-Ramirez, C. G.; Rascon-Cruz, Q.; Herrera-Estrella, L.; Olalde-Portugal, V. Establishment, characterization and plant regeneration from highly chlorophyllous embryogenic cell cultures of blue grama grass, Bouteloua gracilis (H.B.K.) Lag. ex Steud. Plant Cell Rep. 20:131–136; 2001b.
Bhaskaran, S.; Smith, R. H. Regeneration in cereal tissue culture (a review). Crop Sci. 30:1328–1336; 1990.
Bhojwani, S. S.; Razdan, M. K. Plant tissue culture, theory and practice. Amsterdam: Elsevier Science Publisher BV; 1983:592 pp.
Kearney, J. F.; Parrot, W. A.; Hill, N. S. Infection of somatic embryos of tall fescue with Acremonium coenophialum. Crop Sci. 31:979–984; 1991.
Li, L.; Qu, R. In vitro somatic embryogenesis in turf-type bermudagrass: roles of abscisic acid and gibberellic acid and occurrence of secondary somatic embryogenesis. Plant Breeding 121(2):155–158; 2002.
Mckell, C. M.; Goodin, J. R. US arid shrublands in perspective. Arid shrublands—Proc. third workshop of the United States-Australia Rangelands Panel, Tucson, AZ; 1973.
Murashige, T.; Skoog, F. A revised medium for rapid growth and biossays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Osuna-Avila, P.; Nava-Cedillo, A.; Jofre-Garfias, A.; Cabrera-Ponce, J. L. Plant regeneration from shoot apex explants of foxtail millet. Plant Cell Tiss. Organ Cult. 40:33–35; 1995.
Phillips, G. C.; Hubstenberger, J. F.; Hansen, E. E. Plant regeneration by organogenesis from callus and cell suspension cultures. In: Gamborg, O. L.; Phillips, G. C., eds. Plant cell, tissue and organ culture, fundamental methods. Berlin: Springer-Verlag; 1995:67–77.
Sahasrabudhe, N. A.; Nandi, M.; Bahulikan, R. Influence of boric acid on somatic embryogenesis of a cytosterile line of indica rice. Plant Cell Tiss. Organ Cult. 58:73–75; 1999.
Selles, M.; Viladomat, F.; Bastida, J.; Codina, C. Callus induction, somatic embryogenesis and organogenesis in Narcissus confusus: correlation between the state of differentiation and the content of galanthamine and related alkaloids. Plant Cell Rep. 18:646–650; 1999.
Vasil, I. K. Developing cell and tissue culture systems for the improvement of cereal and grass crop. Bio/Technology 6:397–402; 1987.
Vasil, V.; Vasil, I. K. Characterization of an embryogenic cell suspension culture derived from inflorescences of Pennisetum americanum (pearl millet gramineae). Am. J. Bot. 69:1441–1449; 1982.
Wernicke, W.; Brettel, R. Somatic embryogenesis from Sorghum bicolor leaves. Nature 287:138–139; 1980.
Wernicke, W.; Milkovits, L. The regeneration potential of wheat shoot meristems in presence and absence of 2,4-dichlorophenoxyacetic acid. Protoplasma 131:131–141; 1984.
Wernicke, W.; Milkovits, L.; Thomas, E. Morphogenesis from cultured leaf tissue of Sorghum bicolor—the morphogenetic pathways. Protoplasma 111:53–62; 1982.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Osuna, P., Barrow, J.R. Regeneration of black grama (Bouteloua eriopoda torr. torr) plants via somatic embryogenesis. In Vitro Cell.Dev.Biol.-Plant 40, 299–302 (2004). https://doi.org/10.1079/IVP2003533
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1079/IVP2003533