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Callus induction, somatic embryogenesis and organogenesis in Narcissus confusus: correlation between the state of differentiation and the content of galanthamine and related alkaloids

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Abstract

In vitro cultures from two strains of Narcissus confusus (Amaryllidaceae) initiated from mature seeds were screened for their ability to produce alkaloids. Protocols for callus induction, somatic embryogenesis and organogenesis were established. The alkaloid contents were determined by HPLC. Undifferentiated calli produced small amounts of galanthamine, which increased with the degree of tissue differentiation. Scanning electron micrographs of the cultures at different stages of development were made. Embryogenic friable calli were maintained in culture for more than 2 years, retaining a high regeneration capability. All regenerated plants showed normal morphological characteristics.

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Authors and Affiliations

  1. Department of Natural Products, Faculty of Pharmacy, University of Barcelona, Avda. Diagonal 643, E-08028 Barcelona, Catalonia, Spain Fax:+34-3-4029043 e-mail: ccodina@farmacia.far.ub.es, , , , , , ES

    M. Sellés, F. Viladomat, J. Bastida & C. Codina

Authors
  1. M. Sellés
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  2. F. Viladomat
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  3. J. Bastida
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  4. C. Codina
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Received: 20 August 1997 / Revision received: 20 December 1997 / Accepted: 1 February 1998

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Sellés, M., Viladomat, F., Bastida, J. et al. Callus induction, somatic embryogenesis and organogenesis in Narcissus confusus: correlation between the state of differentiation and the content of galanthamine and related alkaloids. Plant Cell Reports 18, 646–651 (1999). https://doi.org/10.1007/s002990050636

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  • DOI: https://doi.org/10.1007/s002990050636

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