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Somatic embryogenesis: A valuable alternative for propagating selected robusta coffee (Coffea canephora) clones

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Summary

The behavior of four coffee (Coffea canephora cv. Robusta) clones was evaluated in this work to define the conditions that would allõw somatic embryogenesis to be induced at different seasons of the year. The objective was to have an adaptive methodology for in vitro propagation of these four coffee clones that were selected for their agronomic characteristics. The use of 2,4-dichlorophenoxyacetic acid and Picloram in the culture medium at concentrations between 2.07 and 4.14 μM resulted in high-frequency somatic embryogenesis when leaves were collected between April and June for genotype C-R, and between April and July for M-229. Callus from 28 to 35d of induction showed better results throughout the whole regeneration process, particularly in liquid medium. There was a clear difference in the behavior of the clones evaluated.

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Authors and Affiliations

  1. Instituto Nacional de Ciencias Agricolas, Km 3.5 Carretera de Tapaste. San José de las Lajas, C.B. 32700, Havana, Cuba

    N. Santana, M. Valcárcel & M. M. Hernández

  2. Centro de Investigación Científica de Yucatán, Calle 43 No. 373 Colonia Chuburná de Hidalgo, 97200, Mérida, Mexico

    N. Santana, A. Canto-Flick, F. Barahona, J. Mijangos-Cortés & V. M. Loyola-Vargas

  3. Estación Central de Investigaciones de Café y Cacao, Cruce de los Baños, C.P. 92700, Tercer Frente, Cuba

    M. E. González

  4. Instituto Tecnológico Agropecuario No. 2, Km 16.3 Carretera Antigua a Motul. Conkal, CP. 97345, Yucatán, Mexico

    F. J. Fuentes-Cerda

Authors
  1. N. Santana
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  2. M. E. González
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  3. M. Valcárcel
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  4. A. Canto-Flick
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  5. M. M. Hernández
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  6. F. J. Fuentes-Cerda
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  7. F. Barahona
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  8. J. Mijangos-Cortés
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  9. V. M. Loyola-Vargas
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Correspondence to V. M. Loyola-Vargas.

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Santana, N., González, M.E., Valcárcel, M. et al. Somatic embryogenesis: A valuable alternative for propagating selected robusta coffee (Coffea canephora) clones. In Vitro Cell.Dev.Biol.-Plant 40, 95–101 (2004). https://doi.org/10.1079/IVP2003486

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  • DOI: https://doi.org/10.1079/IVP2003486

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