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Picloram-induced somatic embryogenesis in pejibaye palm (Bactris gasipaes H.B.K.)

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Abstract

Somatic embryogenesis in pejibaye or peach palm (Bactris gasipaes H.B.K.) was induced from callus derived from in vitro cultured shoot tips of young field-grown plants using a modified Murashige and Skoog medium supplemented with 5 mg L−1 of N6-benzyladenine (BA) and 0.06 mg L−1 of picloram for three months in the dark; this was followed by an additional three months with the same medium and incubation conditions, but using 0.03 mg L−1 of picloram. The cultures were then transferred to light on a medium without hormones. This led to the formation of morphogenic callus, in which somatic embryos, as well as shoot primordia, and finally complete plantlets were formed. These plantlets continued to grow on transfer to the greenhouse.

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References

  1. Arias O (1979) Effecto del flurenol en la produccion de hijos laterales de pejibaye (Bactris gasipaes H.B.K.). Agronomia Costarricense 3: 47–52

    Google Scholar 

  2. Arias O, Huete F (1983) Propagacion vegetativa in vitro del pejibaye (Bactris gasipaes H.B.K.). Turialba 33: 103–108

    Google Scholar 

  3. Beyl CA, Sharma GC (1983) Picloram induced somatic embryogenesis in Gasteria and Haworthia. Plant Cell Tissue Organ Culture 2: 123–132

    Google Scholar 

  4. Brackpool AL, Branton RL, Blake J (1986) Regeneration in palms. In: Vasil IK (ed) Cell Culture and somatic cell genetics of plants, Vol. 3, New York: Academic Press, pp. 207–222

    Google Scholar 

  5. Collins GB, Vian WE, Phillips GC (1978) Use of 4-amino-3,5,6-trichloro picolinic acid as an auxin source in plant tissue cultures. Crop Science 18: 286–288

    Google Scholar 

  6. Chaverri Y (1984) Evaluacion de dosis y fuentes de nitrogeno en el medio de Murashige y Skoog modificado para el cultivo de meristemas de pejibaye (Bactris gasipaes H.B.K.) Tesis Ing. Agr. San Jos, Costa Rica, Facultad de Agronomia, Universidad de Costa Rica. 75 p.

  7. Evans DA, Sharp WR, Flick CE (1981) Growth and behavior of cell cultures: embryogenesis and organogenesis. In: Thorpe TA (ed) Plant Tissue Culture. Methods and applications in agriculture. New York: Academic Press, pp. 45–113

    Google Scholar 

  8. Huang LC, Murashige T (1983) Tissue culture investigations of Bamboo. Bot Bull Academia Sinica 24: 31–52

    Google Scholar 

  9. Mora-Urpi J, Solis E (1980) Polinización en Bactris gasipaes H.B.K. Rev Biol Trop 28: 153–174

    Google Scholar 

  10. Mora-Urpi J (1983) El pejibaye Bactris gasipaes H.B.K.: origen, biologia floral y manejo agronomico. In: Palmeras poco utilizadas en America Tropical. Informe de la Reunion de la Consulta FAO y CATIE. San José, Costa Rica, 172 p

    Google Scholar 

  11. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–487

    Google Scholar 

  12. Phillips GC, Luteyn KJ (1983) Effects of picloram and other auxins on onion tissue culture. J Amer Soc Hort Sci 108: 948–953

    Google Scholar 

  13. Sondahl MR, ZMonaco LC, Sharp WR (1981) In vitro methods applied to coffee. In: Thorpe TA (ed) Plant Tissue Culture. Methods and applications in agriculture. New York: Academic Press, pp 325–347

    Google Scholar 

  14. Stuart DA, Nelsen J, Strickland SG, Niehol JW (1985) Factors affecting developmental processes in alfalfa cell cultures. In: Henke RR, Hughes KW, Constantin MP, Hollaender A (eds) Tissue Culture in Forestry and Agriculture. New York: Plenum, pp 59–73

    Google Scholar 

  15. Thorpe TA (1982) Callus organization and de novo formation of shoots, roots and embryos in vitro. In: Tomes DT, Ellis BE, Harvey PM, Kasha KJ, Peterson RL (eds) Application of Plant Cell and Tissue Culture to Agriculture and Industry, Univ. of Guelph, Ontario, pp 115–138

    Google Scholar 

  16. Tisserat B (1984) Date palm. In: Sharp WR, Evans DA, Ammirato PV, Yamada Y (eds) Handbook of Plant Cell Culture, Vol. 2, New York: Macmillan, pp 505–545

    Google Scholar 

  17. Vasil IK (1985) Somatic embryogenesis and its consequences in the Graminae. In: Henke RR, Hughes RW, Constantin MP, Hollaender A (eds) Tissue culture in forestry and agriculture. New York: Plenum, pp 31–47

    Google Scholar 

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Authors and Affiliations

  1. Tissue Culture Laboratory, Agronomy Research Center, University of Costa Rica, San José, Costa Rica

    Roberto Valverde & Oscar Arias

  2. Plant Physiology Research Group, Department of Biology, University of Calgary, T2N 1N4, Calgary, Alberta, Canada

    Trevor A. Thorpe

Authors
  1. Roberto Valverde
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  2. Oscar Arias
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  3. Trevor A. Thorpe
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Valverde, R., Arias, O. & Thorpe, T.A. Picloram-induced somatic embryogenesis in pejibaye palm (Bactris gasipaes H.B.K.). Plant Cell Tiss Organ Cult 10, 149–156 (1987). https://doi.org/10.1007/BF00035913

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  • DOI: https://doi.org/10.1007/BF00035913

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